Wdr18 (NM_001039027) Rat Tagged ORF Clone

CAT#: RR207559

Wdr18 (Myc-DDK-tagged ORF) - Rat WD repeat domain 18 (Wdr18), (10 ug)

Product Images

Circular map for RR207559

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Wdr18
• Synonyms: MGC124826
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR207559 representing NM_001039027
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCGCCCATGGAAGTGGTGGTGTGTACAGACTCCGCGGCACCGCTATGGAGCTGCATGGTGTGGG
  AGCTTCATTCGGGCGCCAACCTGCTCACCTATCGAGGCGGCCAAGCGGGGCCGCGAGGCCTGGCGCTACT
  CAATGGCGAGTACCTGCTGGCGGCACAGCAGGGCAAGAACTACATCTGCGCTTGGGAGCTGCAGCGCAAG
  GACCAGCTTCAACAGAAAATCATGTGTCCTGGGCCTGTCACCTGTCTAACCACGGCACCTAACGGCTTGT
  ATGTTCTGGCAGGAATTGCAGAGAGCATTTACCTGTGGGAGGTCTCCACGGGGAACCTCCTGGTCATCCT
  CAGCCGTCACTACCAGGATGTGTCGTGCCTGAAGTTCACAGGTGACGGCAGCCACTTTGTCTCTGCAGGC
  AAGGACTGCCTGGTGCTGGCTTGGAGCCTCTGCAGTGTGCTACAGGCAGACCCGTCCAGGATCCTTGCCC
  CAAGACACGTCTGGTCCCAGCACACGCTCCCAATCACAGACCTGCACTGCGGCTTTGGGGGCCCCATGGC
  CCGGGTGGCCACCGCTTCACTGGACCAGACCATGAAGCTCTGGGCCATCTCCTCGGGAGACCTGCTGCTG
  TCTGTCTTGTTTGACATGGGCATCACGTCTGTGACCATGGACCTGGCCGAACACTACATATTCTGTGGGG
  GCAGTGATGGCTCCATCTTCCAGGTGGACCTGTGCAGCCGGCCCGGACCGAGGGAGCAGAGCTTCCAGCC
  CGAGCAGAACACAGGGAAGGTTTTCAAAGGACACAGGAACCAAGTGACATGCCTGTCGGTGTCAACAGAC
  GGCAGCATCCTGCTCTCCGGCTCCCATGACGAGTCAGTGCGGCTGTGGGATGTGAAGAGCAAGCAGTGCG
  TGCGCACAGTCCCCCTCAAAGGCCCAGTGACCAATGCAGCCATCACACTGGCCCCGCCCAGCATGCTGAA
  CCCTGAGTTCAGGCCCAGCCTGCCCCTGCCCCACTTCAACAAGCACCTGCTGGGTGCTGAGCATGGAGAC
  GAAGCTCAGGGTGGGGGCCTGCGCCTGCAGCTGGGGCTCCACCTGCAGGGCAAAGAACCTAGCTACCTGG
  AGCGCCTGGAGCAGCTGCAGGCCGCATTGTCCGGCTATCTGGAGAAGAACATGCTAGGCAGCCAGATGCT
  GCCCGTGCGTGTGTTTGAGCTGGAAGAGGAAGTTCGTAGCCTGCGCAAGATCAACCGGGACCTGTTTGAC
  TTCTCCACACGCATCATCACACGGCCCTCTAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR207559 representing NM_001039027
  Red=Cloning site Green=Tags(s)
  
  MAAPMEVVVCTDSAAPLWSCMVWELHSGANLLTYRGGQAGPRGLALLNGEYLLAAQQGKNYICAWELQRK
  DQLQQKIMCPGPVTCLTTAPNGLYVLAGIAESIYLWEVSTGNLLVILSRHYQDVSCLKFTGDGSHFVSAG
  KDCLVLAWSLCSVLQADPSRILAPRHVWSQHTLPITDLHCGFGGPMARVATASLDQTMKLWAISSGDLLL
  SVLFDMGITSVTMDLAEHYIFCGGSDGSIFQVDLCSRPGPREQSFQPEQNTGKVFKGHRNQVTCLSVSTD
  GSILLSGSHDESVRLWDVKSKQCVRTVPLKGPVTNAAITLAPPSMLNPEFRPSLPLPHFNKHLLGAEHGD
  EAQGGGLRLQLGLHLQGKEPSYLERLEQLQAALSGYLEKNMLGSQMLPVRVFELEEEVRSLRKINRDLFD
  FSTRIITRPSK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001039027
• ORF Size: 1293 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001039027.1, NP_001034116.1
• RefSeq Size: 1387 bp
• RefSeq ORF: 1296 bp
• Locus ID: 314617
• UniProt ID: Q499N3
• Cytogenetics: 7q11
• MW: 47.2 kDa
• Gene Summary: Functions as a component of the Five Friends of Methylated CHTOP (5FMC) complex; the 5FMC complex is recruited to ZNF148 by methylated CHTOP, leading to desumoylation of ZNF148 and subsequent transactivation of ZNF148 target genes (By similarity). Component of the PELP1 complex involved in the nucleolar steps of 28S rRNA maturation and the subsequent nucleoplasmic transit of the pre-60S ribosomal subunit (By similarity).[UniProtKB/Swiss-Prot Function]