Mmp8 (NM_022221) Rat Tagged ORF Clone

CAT#: RR201294

Mmp8 (Myc-DDK-tagged ORF) - Rat matrix metallopeptidase 8 (Mmp8), (10 ug)

Product Images

Circular map for RR201294

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Mmp8
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR201294 representing NM_022221
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTTCACCTGAAGACACTTCCATTTCTGTTCTTCTTCCACACACAGCTTGCCACAGCCCTCCCAGTGC
  CTCCAGAACACCTGGAAGAGAAAAATATGAAAACTGCTGAGAATTACCTACGAAAATTCTACCACTTACC
  AAGCAATCAGTTCCGGTCTGCAAGGAATGCCACTATGATTGCCGAGAAGCTTAAGGAGATGCAGCGCTTC
  TTCGGCTTGCCCGAGACAGGGAAGCCAGATGCAGCTACAATAGAGATAATGGAAAAGCCTCGCTGTGGAG
  TGCCCGACTCTGGTGATTTCTTGCTAACTCCGGGAAGCCCCAAGTGGACACACACTAACCTGACCTACAG
  GATTATAAACCATACCCCACAGATGTCAAAGGCTGAAGTGAAAACAGAAATCGAGAAAGCTTTTAAAATC
  TGGAGTGTGCCATCAACCCTGACCTTCACTGAGACCTTAGAGGGAGAAGCAGACATCAACATTGCTTTCG
  TCTCAAGAGACCATGGTGACAATTCTCCATTTGATGGACCCAATGGAATCCTTGCCCATGCCTTTCAACC
  AGGCCGGGGTATTGGAGGAGATGCACATTTTGATTCAGAAGAAACGTGGACTCAAGACTCCAAGAATTAC
  AACCTGTTTCTCGTGGCTGCTCATGAATTTGGACATTCCTTGGGACTCTCTCACTCCACTGATCCTGGTG
  CCTTGATGTACCCAAACTATGCTTACAGAGAACCCAGCACCTATTCACTACCTCAAGATGATATCAATGG
  AATTCAGACAATCTATGGACCTTCAGACAACCCTGTCCAACCTACTGGACCCAGCACGCCCACAGCCTGT
  GACCCCCACCTGAGATTTGATGCTGCCACCACACTCCGTGGGGAGATTTACTTCTTTAAAGACAAGTACT
  TCTGGAGACGGCATCCCCAGCTGAGAACAGTTGACCTCAATTTCATATCTCTGTTCTGGCCCTTCCTACC
  TAATGGTCTTCAGGCTGCTTATGAAGACTTTGATAGAGACCTAGTTTTCTTATTTAAAGGCAGACAGTAC
  TGGGCTCTAAGTGCCTATGACTTGCAGCAAGGTTACCCCAGAGATATATCCAACTATGGATTCCCAAGGA
  GTGTCCAAGCCATTGATGCAGCTGTTTCCTATAACGGGAAGACATACTTCTTCGTAAACAACCAATGCTG
  GAGATACGACAATCAAAGAAGATCCATGGATCCAGGTTACCCCACTAGCATAGCAAGCGTGTTCCCAGGA
  ATAAACTGTAGAATTGATGCAGTTTTCCAGCAGGATTCCTTCTTCCTCTTCTTCAGTGGACCACAATATT
  TTGCATTTAATCTTGTCAGTCGCAGAGTCACTAGAGTTGCAAGAAGCAATTTATGGCTTAACTGTCCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR201294 representing NM_022221
  Red=Cloning site Green=Tags(s)
  
  MLHLKTLPFLFFFHTQLATALPVPPEHLEEKNMKTAENYLRKFYHLPSNQFRSARNATMIAEKLKEMQRF
  FGLPETGKPDAATIEIMEKPRCGVPDSGDFLLTPGSPKWTHTNLTYRIINHTPQMSKAEVKTEIEKAFKI
  WSVPSTLTFTETLEGEADINIAFVSRDHGDNSPFDGPNGILAHAFQPGRGIGGDAHFDSEETWTQDSKNY
  NLFLVAAHEFGHSLGLSHSTDPGALMYPNYAYREPSTYSLPQDDINGIQTIYGPSDNPVQPTGPSTPTAC
  DPHLRFDAATTLRGEIYFFKDKYFWRRHPQLRTVDLNFISLFWPFLPNGLQAAYEDFDRDLVFLFKGRQY
  WALSAYDLQQGYPRDISNYGFPRSVQAIDAAVSYNGKTYFFVNNQCWRYDNQRRSMDPGYPTSIASVFPG
  INCRIDAVFQQDSFFLFFSGPQYFAFNLVSRRVTRVARSNLWLNCP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_022221
• ORF Size: 1398 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_022221.1, NP_071557.1
• RefSeq Size: 1401 bp
• RefSeq ORF: 1401 bp
• Locus ID: 63849
• UniProt ID: O88766
• Cytogenetics: 8q11
• MW: 53.3 kDa
• Gene Summary: mouse homolog is a neutral metalloproteinase of the fibrillar collagenase family [RGD, Feb 2006]