Mmp8 (NM_022221) Rat Untagged Clone

CAT#: RN201294

Mmp8 (untagged ORF) - Rat matrix metallopeptidase 8 (Mmp8), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Mmp8
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN201294 representing NM_022221
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTTCACCTGAAGACACTTCCATTTCTGTTCTTCTTCCACACACAGCTTGCCACAGCCCTCCCAGTGC
  CTCCAGAACACCTGGAAGAGAAAAATATGAAAACTGCTGAGAATTACCTACGAAAATTCTACCACTTACC
  AAGCAATCAGTTCCGGTCTGCAAGGAATGCCACTATGATTGCCGAGAAGCTTAAGGAGATGCAGCGCTTC
  TTCGGCTTGCCCGAGACAGGGAAGCCAGATGCAGCTACAATAGAGATAATGGAAAAGCCTCGCTGTGGAG
  TGCCCGACTCTGGTGATTTCTTGCTAACTCCGGGAAGCCCCAAGTGGACACACACTAACCTGACCTACAG
  GATTATAAACCATACCCCACAGATGTCAAAGGCTGAAGTGAAAACAGAAATCGAGAAAGCTTTTAAAATC
  TGGAGTGTGCCATCAACCCTGACCTTCACTGAGACCTTAGAGGGAGAAGCAGACATCAACATTGCTTTCG
  TCTCAAGAGACCATGGTGACAATTCTCCATTTGATGGACCCAATGGAATCCTTGCCCATGCCTTTCAACC
  AGGCCGGGGTATTGGAGGAGATGCACATTTTGATTCAGAAGAAACGTGGACTCAAGACTCCAAGAATTAC
  AACCTGTTTCTCGTGGCTGCTCATGAATTTGGACATTCCTTGGGACTCTCTCACTCCACTGATCCTGGTG
  CCTTGATGTACCCAAACTATGCTTACAGAGAACCCAGCACCTATTCACTACCTCAAGATGATATCAATGG
  AATTCAGACAATCTATGGACCTTCAGACAACCCTGTCCAACCTACTGGACCCAGCACGCCCACAGCCTGT
  GACCCCCACCTGAGATTTGATGCTGCCACCACACTCCGTGGGGAGATTTACTTCTTTAAAGACAAGTACT
  TCTGGAGACGGCATCCCCAGCTGAGAACAGTTGACCTCAATTTCATATCTCTGTTCTGGCCCTTCCTACC
  TAATGGTCTTCAGGCTGCTTATGAAGACTTTGATAGAGACCTAGTTTTCTTATTTAAAGGCAGACAGTAC
  TGGGCTCTAAGTGCCTATGACTTGCAGCAAGGTTACCCCAGAGATATATCCAACTATGGATTCCCAAGGA
  GTGTCCAAGCCATTGATGCAGCTGTTTCCTATAACGGGAAGACATACTTCTTCGTAAACAACCAATGCTG
  GAGATACGACAATCAAAGAAGATCCATGGATCCAGGTTACCCCACTAGCATAGCAAGCGTGTTCCCAGGA
  ATAAACTGTAGAATTGATGCAGTTTTCCAGCAGGATTCCTTCTTCCTCTTCTTCAGTGGACCACAATATT
  TTGCATTTAATCTTGTCAGTCGCAGAGTCACTAGAGTTGCAAGAAGCAATTTATGGCTTAACTGTCCATA
  G
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_022221
• Insert Size: 1401 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_022221.1, NP_071557.1
• RefSeq Size: 1401 bp
• RefSeq ORF: 1401 bp
• Locus ID: 63849
• UniProt ID: O88766
• Cytogenetics: 8q11
• Gene Summary: mouse homolog is a neutral metalloproteinase of the fibrillar collagenase family [RGD, Feb 2006]