UDP glucose dehydrogenase (UGDH) (NM_001184701) Human Tagged ORF Clone
CAT#: RG229996
- TrueORF®
UGDH (tGFP-tagged) - Human UDP-glucose 6-dehydrogenase (UGDH), transcript variant 3
ORF Plasmid: DDK
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
"NM_001184701" in other vectors (4)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Symbol | UDP glucose dehydrogenase |
Synonyms | DEE84; EIEE84; GDH; UDP-GlcDH; UDPGDH; UGD |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG229996 representing NM_001184701
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGAAAGGCCGGGCAGCAGATCTGAAGTATATTGAAGCTTGTGCTAGACGCATTGTGCAAAACTCAA ATGGGTACAAAATTGTGACTGAGAAAAGCACAGTTCCAGTGCGGGCAGCAGAAAGTATCCGTCGCATATT TGATGCAAACACAAAACCCAACTTGAATTTACAGGTGCTGTCCAACCCTGAGTTTCTGGCAGAGGGAACA GCCATCAAGGACCTAAAGAACCCAGACAGAGTACTGATTGGAGGGGATGAAACTCCAGAGGGCCAGAGAG CTGTGCAGGCCCTGTGTGCTGTATATGAGCACTGGGTTCCCAGAGAAAAGATCCTCACCACTAATACTTG GTCTTCAGAGCTTTCCAAACTGGCAGCAAATGCTTTTCTTGCCCAGAGAATAAGCAGCATTAACTCCATA AGTGCTCTGTGTGAAGCAACAGGAGCTGATGTAGAAGAGGTAGCAACAGCGATTGGAATGGACCAGAGAA TTGGAAACAAGTTTCTAAAAGCCAGTGTTGGGTTTGGTGGGAGCTGTTTCCAAAAGGATGTTCTGAATTT GGTTTATCTCTGTGAGGCTCTGAATTTGCCAGAAGTAGCTCGTTATTGGCAGCAGGTCATAGACATGAAT GACTACCAGAGGAGGAGGTTTGCTTCCCGGATCATAGATAGTCTGTTTAATACAGTAACTGATAAGAAGA TAGCTATTTTGGGATTTGCATTCAAAAAGGACACTGGTGATACAAGAGAATCTTCTAGTATATATATTAG CAAATATTTGATGGATGAAGGTGCACATCTACATATATATGATCCAAAAGTACCTAGGGAACAAATAGTT GTGGATCTTTCTCATCCAGGTGTTTCAGAGGATGACCAAGTGTCCCGGCTCGTGACCATTTCCAAGGATC CATATGAAGCATGTGATGGTGCCCATGCTGTTGTTATTTGCACTGAGTGGGACATGTTTAAGGAATTGGA TTATGAACGCATTCATAAAAAAATGCTAAAGCCAGCCTTTATCTTCGATGGACGGCGTGTCCTGGATGGG CTCCACAATGAACTACAAACCATTGGCTTCCAGATTGAAACAATTGGCAAAAAGGTGTCTTCAAAGAGAA TTCCATATGCTCCTTCTGGTGAAATTCCGAAGTTTAGTCTTCAAGATCCACCTAACAAGAAACCTAAAGT G ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG229996 representing NM_001184701
Red=Cloning site Green=Tags(s) MGKGRAADLKYIEACARRIVQNSNGYKIVTEKSTVPVRAAESIRRIFDANTKPNLNLQVLSNPEFLAEGT AIKDLKNPDRVLIGGDETPEGQRAVQALCAVYEHWVPREKILTTNTWSSELSKLAANAFLAQRISSINSI SALCEATGADVEEVATAIGMDQRIGNKFLKASVGFGGSCFQKDVLNLVYLCEALNLPEVARYWQQVIDMN DYQRRRFASRIIDSLFNTVTDKKIAILGFAFKKDTGDTRESSSIYISKYLMDEGAHLHIYDPKVPREQIV VDLSHPGVSEDDQVSRLVTISKDPYEACDGAHAVVICTEWDMFKELDYERIHKKMLKPAFIFDGRRVLDG LHNELQTIGFQIETIGKKVSSKRIPYAPSGEIPKFSLQDPPNKKPKV TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_001184701 |
ORF Size | 1191 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001184701.2 |
RefSeq Size | 3026 bp |
RefSeq ORF | 1194 bp |
Locus ID | 7358 |
UniProt ID | O60701 |
Cytogenetics | 4p14 |
Protein Pathways | Amino sugar and nucleotide sugar metabolism, Ascorbate and aldarate metabolism, Metabolic pathways, Pentose and glucuronate interconversions, Starch and sucrose metabolism |
Gene Summary | The protein encoded by this gene converts UDP-glucose to UDP-glucuronate and thereby participates in the biosynthesis of glycosaminoglycans such as hyaluronan, chondroitin sulfate, and heparan sulfate. These glycosylated compounds are common components of the extracellular matrix and likely play roles in signal transduction, cell migration, and cancer growth and metastasis. The expression of this gene is up-regulated by transforming growth factor beta and down-regulated by hypoxia. Alternative splicing results in multiple transcript variants.[provided by RefSeq, May 2010] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC229996 | UGDH (Myc-DDK-tagged)-Human UDP-glucose 6-dehydrogenase (UGDH), transcript variant 3 |
USD 503.00 |
|
RC229996L3 | Lenti-ORF clone of UGDH (Myc-DDK-tagged)-Human UDP-glucose 6-dehydrogenase (UGDH), transcript variant 3 |
USD 803.00 |
|
RC229996L4 | Lenti-ORF clone of UGDH (mGFP-tagged)-Human UDP-glucose 6-dehydrogenase (UGDH), transcript variant 3 |
USD 803.00 |
|
SC328634 | UGDH (untagged)-Human UDP-glucose 6-dehydrogenase (UGDH) transcript variant 3 |
USD 503.00 |
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