CPT1C (NM_001136052) Human Tagged ORF Clone

CAT#: RG226726

CPT1C (tGFP-tagged) - Human carnitine palmitoyltransferase 1C (CPT1C), transcript variant 1

Product Images

Circular map for RG226726

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: CPT1C
• Synonyms: CATL1; CPT1-B; CPT1P; CPTI-B; CPTIC; SPG73
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG226726 representing NM_001136052
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGAAGCGCACCAGGCCGTGGGCTTCCGACCCTCGCTGACCTCGGACGGGGCTGAAGTGGAACTCA
  GTGCCCCTGTGCTGCAGGAGATCTACCTCTCTGGCCTGCGCTCCTGGAAAAGGCATCTCTCACGTTTCTG
  GAATGACTTTCTCACCGGTGTGTTTCCTGCCAGCCCCCTCAGTTGGCTTTTCCTCTTCAGTGCCATCCAG
  CTTGCCTGGTTCCTCCAGCTGGATCCTTCCTTAGGACTGATGGAGAAGATCAAAGAGTTGCTGCCTGACT
  GGGGTGGACAACACCACGGGCTCCGGGGGGTCCTGGCAGCCGCGCTGTTTGCCTCGTGTTTGTGGGGAGC
  CCTGATCTTCACACTGCACGTGGCCCTGAGGCTGCTTCTGTCCTACCACGGCTGGCTTCTTGAGCCCCAC
  GGAGCCATGTCCTCCCCCACCAAGACCTGGCTGGCCCTGGTCCGCATCTTCTCTGGCCGCCACCCGATGC
  TGTTCAGTTACCAGCGCTCCCTGCCACGCCAGCCCGTGCCCTCTGTGCAGGACACCGTGCGCAAGTACCT
  GGAGTCGG[AT]CCGGCCCATCCTCTCCGACGAGGACTTCGACTGGACCGCGGTCCTGGCGCAGGAATTC
  CTGAGGCTGCAGGCGTCGCTGCTGCAGTGGTACCTGCGGCTCAAGTCCTGGTGGGCGTCCAATTATGTCA
  GTGACTGGTGGGAGGAATTTGTGTACCTGCGCTCCCGAAATCCGCTGATGGTGAACAGCAACTATTACAT
  GATGGCAGCTCGCGCTGGGAATGCCGTCCATGCCCTCCTCCTGTACCGCCACCGCCTGAACCGCCAGGAG
  ATACCCCCGACTTTGCTGATGGGAATGCGCCCCTTATGCTCTGCCCAGTACGAGAAGATCTTCAACACCA
  CGCGGATTCCAGGGGTCCAAAAAGACTACATCCGCCACCTCCATGACAGCCAACACGTGGCTGTCTTCCA
  CCGGGGCCGATTCTTCCGCATGGGGACCCACTCCCGAAACAGCCTGCTTTCCCCGAGAGCCCTGGAGCAG
  CAGTTTCAGAGAATCCTGGATGATCCCTCACCGGCCTGCCCCCACGAGGAACATCTGGCAGCTCTGACAG
  CTGCTCCCAGGGGCACGTGGGCCCAGGTGCGGACATCCCTGAAGACCCAGGCAGCGGAGGCCCTGGAGGC
  GGTGGAAGGGGCCGCTTTCTTTGTGTCACTGGATGCTGAGCCCGCGGGGCTCACCAGGGAGGACCCGGCA
  GCGTCGTTGGATGCCTACGCCCATGCTCTGCTGGCCGGCCGGGGCCATGATCGCTGGTTTGACAAATCCT
  TCACCCTAATCGTCTTCTCTAACGGGAAGCTGGGCCTCAGCGTGGAGCACTCCTGGGCCGACTGCCCCAT
  CTCAGGACACATGTGGGAGTTCACTCTGGCTACAGAATGCTTTCAGCTGGGCTACTCAACAGACGGCCAC
  TGCAAGGGGCACCCGGACCCCACACTACCCCAGCCCCAGCGGCTGCAATGGGACCTTCCAGACCAGATCC
  ACTCCTCCATCTCTCTAGCCCTGAGGGGAGCCAAGATCTTGTCTGAAAATGTCGACTGCCATGTCGTTCC
  ATTCTCCCTATTTGGCAAGAGCTTCATCCGACGCTGCCACCTCTCTTCAGACAGCTTCATCCAGATCGCC
  TTGCAACTGGCCCACTTCCGGGACAGGGGTCAATTCTGCCTGACTTATGAGTCGGCCATGACTCGCTTAT
  TCCTGGAAGGCCGGACGGAGACGGTGCGGTCTTGCACGAGGGAGGCCTGCAACTTTGTCAGGGCCATGGA
  GGACAAAGAGAAGACGGACCCACAGTGCCTCGCCCTGTTCCGCGTGGCAGTGGACAAGCACCAGGCTCTG
  CTGAAGGCAGCCATGAGCGGGCAGGGAGTTGACCGCCACCTGTTTGCGCTGTACATCGTGTCCCGATTCC
  TCCACCTGCAGTCGCCCTTCCTGACCCAGGTCCATTCGGAGCAGTGGCAGCTGTCCACCAGCCAGATCCC
  TGTTCAGCAAATGCATCTGTTTGACGTCCACAATTACCCGGACTATGTTTCCTCAGGCGGTGGATTCGGG
  CCTGCTGATGACCATGGTTATGGTGTTTCTTATATCTTCATGGGGGATGGCATGATCACCTTCCACATCT
  CCAGCAAAAAATCAAGCACAAAAACGGATTCCCACAGGCTGGGGCAGCACATTGAGGACGCACTGCTGGA
  TGTGGCCTCCCTGTTCCAGGCGGGACAGCATTTTAAGCGCCGGTTCAGAGGGTCAGGGAAGGAGAACTCC
  AGGCACAGGTGTGGATTTCTCTCCCGCCAGACTGGGGCCTCCAAGGCCTCAATGACATCCACCGACTTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG226726 representing NM_001136052
  Red=Cloning site Green=Tags(s)
  
  MAEAHQAVGFRPSLTSDGAEVELSAPVLQEIYLSGLRSWKRHLSRFWNDFLTGVFPASPLSWLFLFSAIQ
  LAWFLQLDPSLGLMEKIKELLPDWGGQHHGLRGVLAAALFASCLWGALIFTLHVALRLLLSYHGWLLEPH
  GAMSSPTKTWLALVRIFSGRHPMLFSYQRSLPRQPVPSVQDTVRKYLESXXRPILSDEDFDWTAVLAQEF
  LRLQASLLQWYLRLKSWWASNYVSDWWEEFVYLRSRNPLMVNSNYYMMAARAGNAVHALLLYRHRLNRQE
  IPPTLLMGMRPLCSAQYEKIFNTTRIPGVQKDYIRHLHDSQHVAVFHRGRFFRMGTHSRNSLLSPRALEQ
  QFQRILDDPSPACPHEEHLAALTAAPRGTWAQVRTSLKTQAAEALEAVEGAAFFVSLDAEPAGLTREDPA
  ASLDAYAHALLAGRGHDRWFDKSFTLIVFSNGKLGLSVEHSWADCPISGHMWEFTLATECFQLGYSTDGH
  CKGHPDPTLPQPQRLQWDLPDQIHSSISLALRGAKILSENVDCHVVPFSLFGKSFIRRCHLSSDSFIQIA
  LQLAHFRDRGQFCLTYESAMTRLFLEGRTETVRSCTREACNFVRAMEDKEKTDPQCLALFRVAVDKHQAL
  LKAAMSGQGVDRHLFALYIVSRFLHLQSPFLTQVHSEQWQLSTSQIPVQQMHLFDVHNYPDYVSSGGGFG
  PADDHGYGVSYIFMGDGMITFHISSKKSSTKTDSHRLGQHIEDALLDVASLFQAGQHFKRRFRGSGKENS
  RHRCGFLSRQTGASKASMTSTDF
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001136052
• ORF Size: 2379 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001136052.2, NP_001129524.1
• RefSeq Size: 2853 bp
• RefSeq ORF: 2379 bp
• Locus ID: 126129
• UniProt ID: Q8TCG5
• Cytogenetics: 19q13.33
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Adipocytokine signaling pathway, Fatty acid metabolism, PPAR signaling pathway
• Gene Summary: This gene encodes a member of the carnitine/choline acetyltransferase family. The encoded protein regulates the beta-oxidation and transport of long-chain fatty acids into mitochondria, and may play a role in the regulation of feeding behavior and whole-body energy homeostasis. Alternatively spliced transcript variants encoding multiple protein isoforms have been observed for this gene. [provided by RefSeq, Dec 2010]