C7orf59 (LAMTOR4) (NM_001008395) Human Tagged ORF Clone
CAT#: RG209099
- TrueORF®
LAMTOR4 (tGFP-tagged) - Human chromosome 7 open reading frame 59 (C7orf59)
ORF Plasmid: DDK
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"NM_001008395" in other vectors (4)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Symbol | C7orf59 |
Synonyms | C7orf59 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG209099 representing NM_001008395
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACTTCTGCACTGACCCAGGGGCTGGAGCGAATCCCAGACCAGCTCGGCTACCTGGTACTGAGTGAAG GTGCAGTGCTGGCGTCATCTGGGGACCTGGAGAATGATGAGCAGGCAGCCAGTGCCATCTCTGAACTGGT CAGCACAGCCTGCGGTTTCCGGCTGCACCGCGGCATGAATGTGCCCTTCAAGCGCCTGTCTGTGGTCTTT GGAGAACACACACTGCTGGTGACGGTGTCAGGACAGAGGGTGTTTGTGGTGAAGAGGCAGAACCGAGGTC GGGAGCCCATTGATGTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG209099 representing NM_001008395
Red=Cloning site Green=Tags(s) MTSALTQGLERIPDQLGYLVLSEGAVLASSGDLENDEQAASAISELVSTACGFRLHRGMNVPFKRLSVVF GEHTLLVTVSGQRVFVVKRQNRGREPIDV TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_001008395 |
ORF Size | 297 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001008395.1, NP_001008396.1 |
RefSeq Size | 673 bp |
RefSeq ORF | 300 bp |
Locus ID | 389541 |
UniProt ID | Q0VGL1 |
Cytogenetics | 7q22.1 |
Gene Summary | As part of the Ragulator complex it is involved in amino acid sensing and activation of mTORC1, a signaling complex promoting cell growth in response to growth factors, energy levels, and amino acids. Activated by amino acids through a mechanism involving the lysosomal V-ATPase, the Ragulator functions as a guanine nucleotide exchange factor activating the small GTPases Rag. Activated Ragulator and Rag GTPases function as a scaffold recruiting mTORC1 to lysosomes where it is in turn activated.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC209099 | LAMTOR4 (Myc-DDK-tagged)-Human chromosome 7 open reading frame 59 (C7orf59) |
USD 150.00 |
|
RC209099L3 | Lenti ORF clone of Human chromosome 7 open reading frame 59 (C7orf59), Myc-DDK-tagged |
USD 450.00 |
|
RC209099L4 | Lenti ORF clone of Human chromosome 7 open reading frame 59 (C7orf59), mGFP tagged |
USD 450.00 |
|
SC301287 | LAMTOR4 (untagged)-Human chromosome 7 open reading frame 59 (C7orf59) |
USD 165.00 |
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