POLR3B (NM_018082) Human Tagged ORF Clone

CAT#: RG208356

POLR3B (tGFP-tagged) - Human polymerase (RNA) III (DNA directed) polypeptide B (POLR3B), transcript variant 1

Product Images

Circular map for RG208356

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: POLR3B
• Synonyms: C128; HLD8; INMAP; RPC2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG208356 representing NM_018082
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACGTGCTAGCGGAGGAGTTTGGGAACCTGACTCCGGAGCAGCTGGCGGCGCCGATCCCGACTGTAG
  AGGAAAAATGGAGGCTGCTTCCAGCATTTTTAAAGGTGAAAGGCCTTGTGAAACAGCATATAGATTCATT
  TAACTATTTCATTAATGTAGAGATAAAGAAGATAATGAAAGCCAATGAAAAGGTTACAAGTGACGCTGAC
  CCTATGTGGTACTTAAAATATCTTAATATCTATGTTGGGCTTCCTGATGTTGAAGAAAGCTTCAATGTAA
  CTAGACCAGTGTCCCCTCATGAGTGCCGTTTGAGAGACATGACATACTCTGCCCCTATTACAGTGGATAT
  TGAATATACCCGAGGCAGCCAGAGGATCATCCGCAATGCCTTACCTATCGGCAGAATGCCCATAATGCTA
  CGTAGTTCAAACTGTGTTCTTACAGGAAAAACGCCAGCAGAATTTGCCAAACTGAACGAATGTCCCTTAG
  ATCCAGGTGGCTACTTCATTGTTAAAGGAGTAGAAAAAGTTATTCTTATCCAAGAGCAGCTGTCTAAGAA
  CAGGATCATCGTGGAGGCTGATAGAAAAGGGGCTGTTGGAGCTTCAGTTACCAGCTCTACCCATGAGAAA
  AAAAGCAGAACCAATATGGCTGTGAAACAAGGACGATTTTATTTGAGGCATAATACTTTGTCAGAAGATA
  TACCCATTGTCATCATATTTAAGGCCATGGGTGTTGAGAGTGACCAGGAAATTGTGCAGATGATTGGAAC
  AGAGGAGCACGTGATGGCTGCATTTGGGCCCAGTCTGGAAGAGTGCCAGAAAGCTCAGATTTTCACACAG
  ATGCAGGCATTAAAATATATAGGGAACAAAGTAAGAAGGCAAAGGATGTGGGGAGGTGGACCAAAGAAAA
  CCAAAATAGAAGAAGCAAGAGAGCTCCTGGCTTCCACCATTCTGACCCATGTCCCAGTTAAGGAATTCAA
  TTTCCGAGCCAAATGTATCTATACTGCAGTGATGGTGCGAAGAGTTATTCTGGCCCAAGGAGATAATAAA
  GTTGACGACAGAGATTATTATGGTAACAAGCGACTGGAATTGGCAGGACAGCTTTTATCTCTTCTTTTTG
  AAGACTTGTTCAAAAAATTTAATTCTGAAATGAAAAAGATTGCCGACCAGGTGATTCCTAAGCAAAGAGC
  AGCCCAGTTTGATGTTGTCAAACACATGCGCCAAGACCAGATCACCAATGGCATGGTGAATGCTATTTCT
  ACCGGAAATTGGTCTTTAAAGAGATTTAAAATGGACCGCCAGGGTGTAACCCAAGTGCTGTCTCGCTTGT
  CATATATATCCGCACTGGGCATGATGACAAGAATCTCTTCCCAGTTTGAAAAAACGAGAAAAGTGAGTGG
  TCCTCGCTCCCTCCAGCCATCTCAGTGGGGAATGCTGTGTCCTTCGGACACTCCTGAAGGAGAGGCATGT
  GGTTTGGTTAAAAACTTGGCCCTTATGACACACATCACAACTGATATGGAAGATGGACCCATTGTTAAAT
  TAGCCAGTAACTTGGGAGTAGAAGATGTGAATTTATTATGTGGGGAAGAGCTCTCTTACCCAAATGTGTT
  TCTTGTCTTTCTTAATGGTAACATCTTAGGTGTCATTCGAGACCACAAAAAGCTAGTGAATACATTTCGA
  CTCATGAGAAGAGCAGGATATATCAATGAATTTGTTTCCATCTCAACAAATCTTACAGATCGATGTGTCT
  ATATTTCTTCTGATGGGGGAAGGCTATGCAGACCCTACATAATTGTCAAGAAACAGAAGCCAGCAGTCAC
  AAATAAACATATGGAAGAGCTGGCCCAAGGGTACAGGAATTTTGAAGATTTCTTACATGAGAGTCTGGTT
  GAATATTTAGATGTGAATGAAGAAAATGATTGTAACATTGCACTGTACGAACACACAATTAATAAAGACA
  CCACCCACTTGGAGATTGAACCCTTCACTCTTCTCGGCGTGTGTGCTGGACTTATCCCATACCCTCACCA
  TAACCAGTCACCGAGAAACACTTATCAGTGTGCCATGGGGAAACAAGCCATGGGTACTATAGGATACAAC
  CAGCGAAACAGAATTGATACTCTCATGTATCTACTAGCATATCCACAAAAACCCATGGTTAAGACAAAAA
  CCATTGAATTGATAGAATTTGAGAAACTGCCAGCTGGACAGAATGCAACAGTTGCTGTGATGAGCTATAG
  TGGCTATGATATTGAAGATGCTCTTGTTTTAAACAAGGCCTCTTTAGACAGAGGCTTTGGGCGTTGCCTT
  GTATATAAAAATGCTAAATGTACGTTGAAACGATACACCAATCAGACTTTTGATAAAGTGATGGGGCCCA
  TGTTGGATGCTGCTACAAGGAAACCTATCTGGCGACATGAAATCTTAGATGCAGATGGTATTTGTTCTCC
  AGGTGAGAAAGTAGAAAACAAACAAGTGCTTGTAAATAAGTCCATGCCCACAGTGACTCAGATTCCTTTG
  GAAGGAAGTAATGTACCACAGCAACCACAGTACAAAGATGTACCCATAACCTACAAAGGAGCAACAGACT
  CATATATTGAAAAAGTGATGATATCTTCAAATGCTGAAGATGCTTTTCTGATCAAAATGCTGCTGAGACA
  GACAAGGCGTCCAGAAATTGGAGACAAATTCAGCAGTCGTCATGGGCAAAAAGGTGTTTGTGGCTTGATC
  GTCCCCCAGGAAGACATGCCATTTTGTGATTCTGGCATCTGTCCGGACATCATCATGAACCCACACGGCT
  TCCCATCACGAATGACGGTGGGGAAGCTCATTGAGCTGCTGGCTGGCAAGGCCGGTGTGCTGGACGGCAG
  ATTCCACTACGGCACTGCGTTTGGAGGCAGTAAAGTGAAGGATGTGTGTGAGGACCTCGTTCGCCATGGT
  TATAACTACTTGGGGAAAGACTATGTTACATCCGGCATCACAGGTGAGCCCTTAGAAGCATACATCTATT
  TTGGCCCCGTGTACTATCAGAAGCTGAAACACATGGTGCTAGATAAAATGCATGCCCGGGCCCGGGGCCC
  ACGAGCCGTCCTTACCAGGCAACCCACTGAAGGACGGTCTCGTGATGGTGGCTTGCGTCTCGGGGAAATG
  GAACGTGACTGTTTAATCGGTTATGGAGCCAGTATGCTTTTGCTAGAGAGACTAATGATTTCAAGTGATG
  CCTTTGAGGTTGATGTCTGTGGGCAGTGTGGACTTCTGGGGTATTCTGGCTGGTGCCATTACTGCAAGTC
  ATCCTGCCACGTGTCTTCCCTCCGTATTCCGTATGCCTGCAAGCTGCTCTTCCAGGAACTACAGTCTATG
  AACATCATCCCCAGGTTAAAACTGTCCAAGTACAATGAA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG208356 representing NM_018082
  Red=Cloning site Green=Tags(s)
  
  MDVLAEEFGNLTPEQLAAPIPTVEEKWRLLPAFLKVKGLVKQHIDSFNYFINVEIKKIMKANEKVTSDAD
  PMWYLKYLNIYVGLPDVEESFNVTRPVSPHECRLRDMTYSAPITVDIEYTRGSQRIIRNALPIGRMPIML
  RSSNCVLTGKTPAEFAKLNECPLDPGGYFIVKGVEKVILIQEQLSKNRIIVEADRKGAVGASVTSSTHEK
  KSRTNMAVKQGRFYLRHNTLSEDIPIVIIFKAMGVESDQEIVQMIGTEEHVMAAFGPSLEECQKAQIFTQ
  MQALKYIGNKVRRQRMWGGGPKKTKIEEARELLASTILTHVPVKEFNFRAKCIYTAVMVRRVILAQGDNK
  VDDRDYYGNKRLELAGQLLSLLFEDLFKKFNSEMKKIADQVIPKQRAAQFDVVKHMRQDQITNGMVNAIS
  TGNWSLKRFKMDRQGVTQVLSRLSYISALGMMTRISSQFEKTRKVSGPRSLQPSQWGMLCPSDTPEGEAC
  GLVKNLALMTHITTDMEDGPIVKLASNLGVEDVNLLCGEELSYPNVFLVFLNGNILGVIRDHKKLVNTFR
  LMRRAGYINEFVSISTNLTDRCVYISSDGGRLCRPYIIVKKQKPAVTNKHMEELAQGYRNFEDFLHESLV
  EYLDVNEENDCNIALYEHTINKDTTHLEIEPFTLLGVCAGLIPYPHHNQSPRNTYQCAMGKQAMGTIGYN
  QRNRIDTLMYLLAYPQKPMVKTKTIELIEFEKLPAGQNATVAVMSYSGYDIEDALVLNKASLDRGFGRCL
  VYKNAKCTLKRYTNQTFDKVMGPMLDAATRKPIWRHEILDADGICSPGEKVENKQVLVNKSMPTVTQIPL
  EGSNVPQQPQYKDVPITYKGATDSYIEKVMISSNAEDAFLIKMLLRQTRRPEIGDKFSSRHGQKGVCGLI
  VPQEDMPFCDSGICPDIIMNPHGFPSRMTVGKLIELLAGKAGVLDGRFHYGTAFGGSKVKDVCEDLVRHG
  YNYLGKDYVTSGITGEPLEAYIYFGPVYYQKLKHMVLDKMHARARGPRAVLTRQPTEGRSRDGGLRLGEM
  ERDCLIGYGASMLLLERLMISSDAFEVDVCGQCGLLGYSGWCHYCKSSCHVSSLRIPYACKLLFQELQSM
  NIIPRLKLSKYNE
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_018082
• ORF Size: 3399 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_018082.6
• RefSeq Size: 4239 bp
• RefSeq ORF: 3402 bp
• Locus ID: 55703
• UniProt ID: Q9NW08
• Cytogenetics: 12q23.3
• Domains: RNA_pol_Rpb2_6, RNA_pol_Rpb2_7, RNA_pol_Rpb2_2, RNA_pol_Rpb2_1, RNA_pol_Rpb2_3, RNA_pol_Rpb2_4, RNA_pol_Rpb2_5
• Protein Families: Transcription Factors
• Protein Pathways: Cytosolic DNA-sensing pathway, Metabolic pathways, Purine metabolism, Pyrimidine metabolism, RNA polymerase
• Gene Summary: This gene encodes the second largest subunit of RNA polymerase III, the polymerase responsible for synthesizing transfer and small ribosomal RNAs in eukaryotes. The largest subunit and the encoded protein form the catalytic center of RNA polymerase III. Mutations in this gene are a cause of hypomyelinating leukodystrophy. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2011]