KLHL25 (NM_022480) Human Tagged ORF Clone

CAT#: RG206979

KLHL25 (tGFP-tagged) - Human kelch-like 25 (Drosophila) (KLHL25)

Product Images

Circular map for RG206979

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: KLHL25
• Synonyms: ENC-2; ENC2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG206979 representing NM_022480
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGGTCAGTGTCCATGAGACCCGCAAGTCGCGGAGCAGCACGGGGTCCATGAACGTCACCCTCTTCC
  ACAAGGCCTCCCACCCGGACTGTGTGCTGGCCCACCTCAACACGCTTCGCAAGCACTGCATGTTCACCGA
  CGTCACACTCTGGGCGGGCGACCGTGCCTTCCCCTGTCACCGTGCCGTGCTGGCCGCCTCTAGCCGCTAT
  TTTGAGGCCATGTTCAGCCATGGCCTTCGGGAGAGCCGGGATGACACTGTCAACTTCCAGGACAACCTGC
  ACCCGGAGGTGCTGGAGCTGCTGCTGGACTTTGCCTACTCCTCACGCATCGCCATCAACGAGGAGAACGC
  TGAGTCACTGCTGGAGGCAGGCGACATGCTGCAGTTCCACGATGTGCGGGATGCTGCCGCCGAGTTCCTG
  GAGAAGAACCTTTTCCCCTCCAACTGCCTGGGCATGATGCTGCTCTCGGACGCCCACCAGTGCCGCCGGC
  TGTATGAGTTCTCCTGGCGCATGTGCCTGGTGCACTTTGAGACGGTGAGGCAGAGCGAGGACTTCAACAG
  CCTGTCCAAGGACACACTGCTGGACCTCATCTCGAGTGATGAGCTGGAGACCGAGGACGAGCGGGTGGTC
  TTCGAGGCCATCCTCCAGTGGGTGAAGCACGACCTGGAGCCACGGAAGGTCCACTTGCCCGAGCTCCTCC
  GCAGCGTGCGTCTGGCCTTGCTGCCGTCCGACTGCCTGCAGGAGGCCGTCTCCAGCGAGGCCCTCCTCAT
  GGCAGACGAGCGCACCAAGCTTATCATGGATGAGGCCCTGCGCTGCAAGACCAGGATCCTGCAGAATGAT
  GGCGTGGTCACCAGCCCCTGTGCCCGGCCACGCAAGGCGGGCCACACGCTACTCATCCTGGGGGGCCAGA
  CCTTCATGTGTGACAAGATCTACCAGGTGGACCACAAGGCCAAGGAGATCATCCCCAAGGCCGACCTGCC
  CAGCCCCCGGAAGGAGTTCAGCGCCTCAGCGATCGGCTGCAAGGTCTATGTGACGGGGGGCAGGGGCTCC
  GAGAACGGGGTCTCCAAGGATGTCTGGGTGTACGACACCGTACATGAGGAATGGTCCAAGGCGGCGCCCA
  TGCTGATTGCCCGCTTTGGCCATGGCTCAGCTGAGCTGGAGAACTGCCTCTATGTGGTGGGGGGACACAC
  ATCCCTGGCAGGGGTCTTCCCGGCCTCGCCTTCTGTCTCCCTGAAACAAGTGGAGAAATACGACCCTGGG
  GCCAACAAGTGGATGATGGTGGCCCCCTTGCGGGATGGCGTCAGCAATGCCGCAGTGGTGAGTGCCAAGC
  TGAAGCTCTTTGTTTTCGGAGGAACCAGCATCCACCGGGACATGGTGTCCAAGGTCCAGTGCTATGACCC
  CTCGGAGAACAGGTGGACGATCAAGGCCGAGTGCCCCCAGCCTTGGCGGTACACAGCCGCTGCCGTCCTG
  GGCAGCCAGATCTTCATCATGGGAGGTGACACGGAATTCACAGCCGCCTCGGCCTACCGCTTTGACTGTG
  AGACCAACCAGTGGACGCGGATTGGGGACATGACTGCCAAGCGCATGTCCTGCCATGCCCTGGCTTCCGG
  CAACAAGCTCTATGTGGTCGGGGGCTACTTTGGGACCCAGAGGTGTAAGACTCTGGACTGCTATGACCCC
  ACTTCAGATACATGGAACTGCATCACCACAGTGCCCTACTCACTTATCCCCACGGCCTTTGTCAGCACCT
  GGAAGCACCTGCCCGCG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG206979 representing NM_022480
  Red=Cloning site Green=Tags(s)
  
  MSVSVHETRKSRSSTGSMNVTLFHKASHPDCVLAHLNTLRKHCMFTDVTLWAGDRAFPCHRAVLAASSRY
  FEAMFSHGLRESRDDTVNFQDNLHPEVLELLLDFAYSSRIAINEENAESLLEAGDMLQFHDVRDAAAEFL
  EKNLFPSNCLGMMLLSDAHQCRRLYEFSWRMCLVHFETVRQSEDFNSLSKDTLLDLISSDELETEDERVV
  FEAILQWVKHDLEPRKVHLPELLRSVRLALLPSDCLQEAVSSEALLMADERTKLIMDEALRCKTRILQND
  GVVTSPCARPRKAGHTLLILGGQTFMCDKIYQVDHKAKEIIPKADLPSPRKEFSASAIGCKVYVTGGRGS
  ENGVSKDVWVYDTVHEEWSKAAPMLIARFGHGSAELENCLYVVGGHTSLAGVFPASPSVSLKQVEKYDPG
  ANKWMMVAPLRDGVSNAAVVSAKLKLFVFGGTSIHRDMVSKVQCYDPSENRWTIKAECPQPWRYTAAAVL
  GSQIFIMGGDTEFTAASAYRFDCETNQWTRIGDMTAKRMSCHALASGNKLYVVGGYFGTQRCKTLDCYDP
  TSDTWNCITTVPYSLIPTAFVSTWKHLPA
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_022480
• ORF Size: 1767 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_022480.4
• RefSeq Size: 3683 bp
• RefSeq ORF: 1770 bp
• Locus ID: 64410
• UniProt ID: Q9H0H3
• Cytogenetics: 15q25.3
• Domains: BTB, Kelch
• Gene Summary: Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex required for translational homeostasis. The BCR(KLHL25) ubiquitin ligase complex acts by mediating ubiquitination of hypophosphorylated EIF4EBP1 (4E-BP1): ubiquitination and subsequent degradation of hypophosphorylated EIF4EBP1 (4E-BP1) probably serves as a homeostatic mechanism to maintain translation and prevent eIF4E inhibition when eIF4E levels are low. The BCR(KLHL25) complex does not target EIF4EBP1 (4E-BP1) when it is hyperphosphorylated or associated with eIF4E.[UniProtKB/Swiss-Prot Function]