JAKMIP2 (NM_001282282) Human Tagged ORF Clone

CAT#: RC239544

JAKMIP2 (myc-DDK-tagged) - Human janus kinase and microtubule interacting protein 2 (JAKMIP2), transcript variant 4

Product Images

Circular map for RC239544

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: JAKMIP2
• Synonyms: JAMIP2; NECC1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC239544 representing NM_001282282
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTATCAAAGCTTGAAAGAGAGAAGACTCAAGAAGCGAAGAGGATTCGTGAGCTGGAGCAGCGCAAGC
  ACACGGTGCTGGTGACAGAACTCAAAGCCAAGCTCCATGAGGAGAAGATGAAGGAGCTGCAGGCTGTGAG
  GGAGAACCTTATCAAGCAGCACGAGCAGGAAATGTCAAGGACGGTGAAGGTACGTGATGGAGAGATCCAG
  AGGCTCAAGTCTGCTCTCTGTGCTCTCCGCGACGGCAGCAGTGACAAAGTAAGGACAGCGCTCACCATTG
  AGGCCCGGGAGGAGGCCCGGAAACTGTTTGACACAGAGCGCCTTAAGCTCTTACAGGAAATTGCGGACCT
  GAAAACGGCCAAGAAGCAGGTGGACGAGGCTCTGAGCAATATGATCCAAGCAGATAAAATCAAGGCTGGG
  GACCTTCGGAGTGAGCATCAGTCCCACCAAGAAGCCATCTCGAAGATCAAGTGGGAGTCGGAGCGGGATA
  TTCGGAGGCTGATGGATGAAATCAAAGCCAAGGACAGGATCATCTTTTCCCTGGAAAAGGAACTGGAGAC
  CCAGACAGGCTATGTACAGAAACTCCAACTTCAGAAGGAGGCTTTGGACGAACAACTCTTTCTGGTCAAG
  GAGGCTGAGTGCAACATGAGCAGCCCAAAACGAGAAATTCCAGGAAGGGCAGGTGATGGTTCCGAACACT
  GCAGCAGTCCTGATTTGCGAAGAAATCAAAAGAGAATAGCTGAATTGAATGCCACTATAAGAAAATTAGA
  AGACAGGAATACCTTGCTTGGAGATGAACGAAATGAACTGTTAAAACGTGTGCGGGAAACCGAAAAGCAA
  TGTAAACCTCTCCTGGAAAGGAACAAGTGCCTCGCCAAGAGAAACGATGAACTGATGGTGTCCTTGCAGC
  GCATGGAAGAAAAACTAAAAGCCGTTACCAAGGAAAATTCAGAAATGAGAGAAAAAATAACATCCCATCC
  ACCCCTGAAGAAATTAAAATCTCTGAATGACCTCGACCAAGCTAATGAAGAACAAGAAACAGAGTTTCTA
  AAACTTCAGGTCATTGAGCAACAGAACATTATTGATGAGCTCACAAGGGACCGAGAAAAGCTCATCCGTA
  GAAGAAAGCATAGAAGAAGTTCCAAGCCAATTAAGAGGCCTGTTTTGGACCCGTTTATTGGCTATGATGA
  GGACTCTATGGATTCAGAGACATCATCCATGGCCTCATTTAGAACAGACAGAACACCAGCTACTCCTGAT
  GATGACTTGGATGAAAGTTTAGCAGCTGAAGAATCTGAACTAAGATTTCGACAATTAACAAAAGAATATC
  AGGCCCTCCAAAGAGCATATGCCCTCCTACAGGAGCAGACGGGAGGCATCATCGACGCTGAACGAGAAGC
  CAAGGCTCAAGAACAGCTCCAAGCAGAGGTGCTAAGGTATAAAGCCAAAATTGAAGACCTGGAAGCGACT
  CTGGCTCAGAAAGGGCAGGATTCACACTGGGTAGAAGATAAACAACTTTTCATTAAGAGAAACCAGGAGC
  TTTTAGAAAAGATAGAAAAACAGGAGGCAGAAAATCACCGGTTACAACAAGAACTACAGGACGCCAGAGA
  CCAGAATGAGCTGCTGGAGTTTCGAAACCTAGAGCTAGAAGAGAGAGAGAGACGATCCCCTCCATTTAAT
  CTCCAAATTCACCCATTCTCAGATGGTGTGAGTGCTCTACAGATCTACTGTATGAAAGAAGGTGTTAAGG
  ATGTGAACATCCCTGATCTCATAAAGCAGCTTGATATCTTGGGTGATAATGGGAATTTAAGAAATGAAGA
  ACAAGTGGCCATAATTCAGGCCAGCACTGTGCTGTCCCTGGCAGAGAAGTGGATCCAGCAGATTGAAGGA
  GCTGAGGCTGCCCTACACCAGAAAATGATGGAATTGGAAAGTGACATGGAACAGTTCTGCAAAATAAAAG
  GCTATCTGGAGGAAGAACTAGACTACAGAAAACAAGCTCTTGACCAAGCATATATGAGAATCCAGGAACT
  AGAAGCTACTTTGTACAATGCTCTACAGCAAGAAACTGTTATCAAGTTTGGTGAATTATTAAGTGAAAAA
  CAGCAAGAGGAGCTGAGGACGGCAGTAGAAAAGTTACGGCGGCAAATGCTGAGGAAGAGCAGAGAGTATG
  ACTGTCAGATTCTTCAGGAGAGAATGGAGCTCTTACAGCAAGCCCATCAGAGAATTCGTGACTTAGAAGA
  TAAAACAGACATCCAGAAAAGACAAATAAAAGACTTAGAAGAAAAGTTTCTGTTTCTATTCTTGTTCTTC
  TCTCTTGCCTTTATTCTATGGCCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC239544 representing NM_001282282
  Red=Cloning site Green=Tags(s)
  
  MVSKLEREKTQEAKRIRELEQRKHTVLVTELKAKLHEEKMKELQAVRENLIKQHEQEMSRTVKVRDGEIQ
  RLKSALCALRDGSSDKVRTALTIEAREEARKLFDTERLKLLQEIADLKTAKKQVDEALSNMIQADKIKAG
  DLRSEHQSHQEAISKIKWESERDIRRLMDEIKAKDRIIFSLEKELETQTGYVQKLQLQKEALDEQLFLVK
  EAECNMSSPKREIPGRAGDGSEHCSSPDLRRNQKRIAELNATIRKLEDRNTLLGDERNELLKRVRETEKQ
  CKPLLERNKCLAKRNDELMVSLQRMEEKLKAVTKENSEMREKITSHPPLKKLKSLNDLDQANEEQETEFL
  KLQVIEQQNIIDELTRDREKLIRRRKHRRSSKPIKRPVLDPFIGYDEDSMDSETSSMASFRTDRTPATPD
  DDLDESLAAEESELRFRQLTKEYQALQRAYALLQEQTGGIIDAEREAKAQEQLQAEVLRYKAKIEDLEAT
  LAQKGQDSHWVEDKQLFIKRNQELLEKIEKQEAENHRLQQELQDARDQNELLEFRNLELEERERRSPPFN
  LQIHPFSDGVSALQIYCMKEGVKDVNIPDLIKQLDILGDNGNLRNEEQVAIIQASTVLSLAEKWIQQIEG
  AEAALHQKMMELESDMEQFCKIKGYLEEELDYRKQALDQAYMRIQELEATLYNALQQETVIKFGELLSEK
  QQEELRTAVEKLRRQMLRKSREYDCQILQERMELLQQAHQRIRDLEDKTDIQKRQIKDLEEKFLFLFLFF
  SLAFILWP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001282282
• ORF Size: 2334 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001282282.2
• RefSeq Size: 8995 bp
• RefSeq ORF: 2337 bp
• Locus ID: 9832
• UniProt ID: Q96AA8
• Cytogenetics: 5q32
• MW: 92 kDa
• Gene Summary: The protein encoded by this gene is reported to be a component of the Golgi matrix. It may act as a golgin protein by negatively regulating transit of secretory cargo and by acting as a structural scaffold of the Golgi. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012]