ARNTL2 (NM_001248003) Human Tagged ORF Clone

CAT#: RC234524

ARNTL2 (Myc-DDK tagged) - Homo sapiens aryl hydrocarbon receptor nuclear translocator-like 2 (ARNTL2), transcript variant 3

Product Images

Circular map for RC234524

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ARNTL2
• Synonyms: bHLHe6; BMAL2; CLIF; MOP9; PASD9
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC234524 representing NM_001248003
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCGGAAGAGGAGGCTGCGGCGGGAGGTGAGGTTGCCGGTGGCGAGGCGACGGCCCCAGGTAAAG
  TGTTGAGAGAGGAGAACCAGTGCATTGCTCCTGTGGTTTCCAGCCGCGTGAGTCCAGGGACAAGACCAAC
  AGCTATGGGGTCTTTCAGCTCACACATGACAGAGTTTCCACGAAAACGCAAAGGAAGTGATTCAGACCCA
  TCCCAAGAAGCTCATAGCCAAACTGAAAAGCGGAGGAGAGATAAAATGAATAACCTGATTGAAGAACTGT
  CTGCAATGATCCCTCAGTGCAACCCCATGGCGCGTAAACTGGACAAACTTACAGTTTTAAGAATGGCTGT
  TCAACACTTGAGATCTTTAAAAGGCTTGACAAATTCTTATGTGGGAAGTAATTATAGACCATCATTTCTT
  CAGGATAATGAGCTCAGACATTTAATCCTTAAGACTGCAGAAGGCTTCTTATTTGTGGTTGGATGTGAAA
  GAGGAAAAATTCTCTTCGTTTCTAAGTCAGTCTCCAAAATACTTAATTATGATCAGGCTAGTTTGACTGG
  ACAAAGCTTATTTGACTTCTTACATCCAAAAGATGTTGCCAAAGTAAAGGAACAACTTTCTTCTTTTGAT
  ATTTCACCAAGAGAAAAGCTAATAGATGCCAAAACTGGTTTGCAAGTTCACAGTAATCTCCACGCTGGAA
  GGACACGTGTGTATTCTGGCTCAAGACGATCTTTTTTCTGTCGGATAAAGAGTTGTAAAATCTCTGTCAA
  AGAAGAGCATGGATGCTTACCCAACTCAAAGAAGAAAGAGCACAGAAAATTCTATACTATCCATTGCACT
  GGTTACTTGAGAAGCTGGCCTCCAAATATTGTTGGAATGGAAGAAGAAAGGAACAGTAAGAAAGACAACA
  GTAATTTTACCTGCCTTGTGGCCATTGGAAGATTACAGCCATATATTGTTCCACAGAACAGTGGAGAGAT
  TAATGTGAAACCAACTGAATTTATAACCCGGTTTGCAGTGAATGGAAAATTTGTCTATGTAGATCAAAGG
  GCAACAGCGATTTTAGGATATCTGCCTCAGGAACTTTTGGGAACTTCTTGTTATGAATATTTTCATCAAG
  ATGACCACAATAATTTGACTGACAAGCACAAAGCAGTTCTACAGAGTAAGGAGAAAATACTTACAGATTC
  CTACAAATTCAGAGCAAAAGATGGCTCTTTTGTAACTTTAAAAAGCCAATGGTTTAGTTTCACAAATCCT
  TGGACAAAAGAACTGGAATATATTGTATCTGTCAACACTTTAGTTTTGGGACATAGTGAGCCTGGAGAAG
  CATCATTTTTACCTTGTAGCTCTCAATCATCAGAAGAATCCTCTAGACAGTCCTGTATGAGTGTACCTGG
  AATGTCTACTGGAACAGTACTTGGTGCTGGTAGTATTGGAACAGATATTGCAAATGAAATTCTGGATTTA
  CAGAGGTTACAGTCTTCTTCATACCTTGATGATTCGAGTCCAACAGGTTTAATGAAAGATACTCATACTG
  TAAACTGCAGGAGTATGTCAAATAAGGAGTTGTTTCCACCAAGTCCTTCTGAAATGGGGGAGCTAGAGGC
  TACCAGGCAAAACCAGAGTACTGTTGCTGTCCACAGCCATGAGCCACTCCTCAGTGATGGTGCACAGTTG
  GATTTCGATGCCCTATGTGACAATGATGACACAGCCATGGCTGCATTTATGAATTACTTAGAAGCAGAGG
  GGGGCCTGGGAGACCCTGGGGACTTCAGTGACATCCAGTGGACCCTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC234524 representing NM_001248003
  Red=Cloning site Green=Tags(s)
  
  MAAEEEAAAGGEVAGGEATAPGKVLREENQCIAPVVSSRVSPGTRPTAMGSFSSHMTEFPRKRKGSDSDP
  SQEAHSQTEKRRRDKMNNLIEELSAMIPQCNPMARKLDKLTVLRMAVQHLRSLKGLTNSYVGSNYRPSFL
  QDNELRHLILKTAEGFLFVVGCERGKILFVSKSVSKILNYDQASLTGQSLFDFLHPKDVAKVKEQLSSFD
  ISPREKLIDAKTGLQVHSNLHAGRTRVYSGSRRSFFCRIKSCKISVKEEHGCLPNSKKKEHRKFYTIHCT
  GYLRSWPPNIVGMEEERNSKKDNSNFTCLVAIGRLQPYIVPQNSGEINVKPTEFITRFAVNGKFVYVDQR
  ATAILGYLPQELLGTSCYEYFHQDDHNNLTDKHKAVLQSKEKILTDSYKFRAKDGSFVTLKSQWFSFTNP
  WTKELEYIVSVNTLVLGHSEPGEASFLPCSSQSSEESSRQSCMSVPGMSTGTVLGAGSIGTDIANEILDL
  QRLQSSSYLDDSSPTGLMKDTHTVNCRSMSNKELFPPSPSEMGELEATRQNQSTVAVHSHEPLLSDGAQL
  DFDALCDNDDTAMAAFMNYLEAEGGLGDPGDFSDIQWTL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001248003
• ORF Size: 1797 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001248003.2
• RefSeq Size: 7300 bp
• RefSeq ORF: 1800 bp
• Locus ID: 56938
• UniProt ID: Q8WYA1
• Cytogenetics: 12p11.23
• Protein Families: Druggable Genome, Transcription Factors
• MW: 66.9 kDa
• Gene Summary: This gene encodes a basic helix-loop-helix transcription factor belonging to the PAS (PER, ARNT, SIM) superfamily. The PAS proteins play important roles in adaptation to low atmospheric and cellular oxygen levels, exposure to certain environmental pollutants, and diurnal oscillations in light and temperature. This protein forms a transcriptionally active heterodimer with the circadian CLOCK protein, the structurally related MOP4, and hypoxia-inducible factors, such as HIF1alpha. Consistent with its role as a biologically relevant partner of circadian and hypoxia factors, this protein is coexpressed in regions of the brain such as the thalamus, hypothalamus, and amygdala. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Oct 2011]