C1orf25 (TRMT1L) (NM_001202423) Human Tagged ORF Clone

CAT#: RC232988

TRMT1L (Myc-DDK tagged) - Homo sapiens tRNA methyltransferase 1 homolog (S. cerevisiae)-like (TRMT1L), transcript variant 2

Product Images

Circular map for RC232988

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: C1orf25
• Synonyms: bG120K12.3; C1orf25; MST070; MSTP070; TRM1L
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC232988 representing NM_001202423
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGCATCTGTCACTTACCTTGTCGACCAGTGAAACCAAACATTATTGGAGAACAGATAACCAGTAAAA
  TGGGAGCCCATTATCATTGTATCATTTGTTCAGCAACAATCACCAGAAGAACTGATATGCTAGGACATGT
  TAGGCGCCACATGAATAAAGGAGAGACTAAATCTAGTTATATTGCAGCTTCCACTGCTAAACCACCTAAG
  GAAATTTTGAAAGAGGCAGACACGGATGTACAAGTTTGTCCCAACTATTCTATACCTCAGAAAACAGATT
  CCTATTTTAACCCCAAAATGAAACTAAATCGGCAGCTAATATTCTGTACATTGGCTGCTTTGGCTGAGGA
  ACGAAAACCTTTGGAATGTCTAGATGCTTTTGGAGCCACTGGGATAATGGGATTACAGTGGGCAAAACAT
  CTTGGAAATGCAGTCAAAGTTACAATCAATGACTTGAATGAAAATTCTGTGACACTGATTCAGGAAAACT
  GCCATTTAAACAAATTGAAAGTGGTGGTGGACAGTAAGGAAAAGGAAAAGAGTGATGATATTCTTGAAGA
  AGGAGAGAAAAATCTTGGTAATATTAAGGTGACCAAAATGGATGCCAATGTACTGATGCATTTGAGATCT
  TTTGATTTCATACATCTAGACCCTTTTGGAACATCAGTGAATTATCTAGATTCTGCATTCAGAAATATAA
  GAAACCTTGGCATAGTGTCAGTGACTTCTACAGATATCAGTTCTTTATATGCCAAGGCACAGCATGTTGC
  CCGGCGTCACTACGGATGTAACATTGTCCGAACTGAATATTACAAGGAACTAGCAGCCAGAATTGTTGTA
  GCTGCAGTGGCAAGAGCTGCAGCCCGATGCAACAAAGGCATAGAAGTACTGTTTGCAGTGGCTCTGGAAC
  ATTTTGTGTTGGTAGTTGTGAGAGTTTTGAGGGGACCTACTTCAGCAGATGAAACAGCCAAGAAGATTCA
  ATACCTGATCCATTGTCAGTGGTGTGAAGAGAGAATTTTTCAGAAGGATGGTAATATGGTAGAAGAAAAC
  CCATATAGACAGCTGCCTTGTAACTGTCATGGAAGCATGCCTGGAAAGACAGCAATAGAACTTGGACCTC
  TGTGGTCAAGTTCCCTTTTCAATACTGGATTCCTCAAAAGAATGCTATTTGAATCTCTTCACCATGGTTT
  GGATGACATTCAGACCCTAATAAAGACATTAATCTTTGAATCAGAGTGTACGCCTCAAAGTCAGTTTTCA
  ATTCATGCATCTTCAAATGTCAACAAGCAAGAAGAAAATGGTGTATTTATTAAAACTACAGATGACACCA
  CAACAGATAATTACATTGCACAAGGAAAGAGAAAAAGTAATGAAATGATCACAAATTTAGGCAAGAAGCA
  AAAGACTGATGTCAGTACTGAACATCCTCCCTTTTATTACAACATTCACAGACACAGCATTAAAGGAATG
  AATATGCCAAAGTTAAAAAAGTTTTTGTGCTATTTATCTCAAGCAGGCTTTCGAGTAAGCCGAACTCATT
  TTGACCCAATGGGTGTACGCACAGATGCACCTCTGATGCAGTTTAAATCTATCCTTTTAAAGTACAGCAC
  CCCCACCTACACTGGAGGACAGTCAGAAAGCCATGTCCAGTCAGCATCTGAAGATACAGTAACTGAAAGA
  GTTGAAATGTCAGTGAATGACAAAGCAGAAGCAAGTGGCTGCAGAAGATGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC232988 representing NM_001202423
  Red=Cloning site Green=Tags(s)
  
  MCICHLPCRPVKPNIIGEQITSKMGAHYHCIICSATITRRTDMLGHVRRHMNKGETKSSYIAASTAKPPK
  EILKEADTDVQVCPNYSIPQKTDSYFNPKMKLNRQLIFCTLAALAEERKPLECLDAFGATGIMGLQWAKH
  LGNAVKVTINDLNENSVTLIQENCHLNKLKVVVDSKEKEKSDDILEEGEKNLGNIKVTKMDANVLMHLRS
  FDFIHLDPFGTSVNYLDSAFRNIRNLGIVSVTSTDISSLYAKAQHVARRHYGCNIVRTEYYKELAARIVV
  AAVARAAARCNKGIEVLFAVALEHFVLVVVRVLRGPTSADETAKKIQYLIHCQWCEERIFQKDGNMVEEN
  PYRQLPCNCHGSMPGKTAIELGPLWSSSLFNTGFLKRMLFESLHHGLDDIQTLIKTLIFESECTPQSQFS
  IHASSNVNKQEENGVFIKTTDDTTTDNYIAQGKRKSNEMITNLGKKQKTDVSTEHPPFYYNIHRHSIKGM
  NMPKLKKFLCYLSQAGFRVSRTHFDPMGVRTDAPLMQFKSILLKYSTPTYTGGQSESHVQSASEDTVTER
  VEMSVNDKAEASGCRRW
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001202423
• ORF Size: 1731 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001202423.1, NP_001189352.1
• RefSeq Size: 4029 bp
• RefSeq ORF: 1734 bp
• Locus ID: 81627
• UniProt ID: Q7Z2T5
• Cytogenetics: 1q25.3
• MW: 65.5 kDa
• Gene Summary: This gene encodes a protein that has some similarity to N2,N2-dimethylguanosine tRNA methyltransferase from other organisms. Studies of the mouse ortholog have shown that this protein plays a role in motor coordination and exploratory behavior, and it may also be involved in modulating postnatal neuronal functions. Alternatively spliced transcripts have been identified for this gene. [provided by RefSeq, Jan 2011]