WDR43 (NM_015131) Human Tagged ORF Clone

CAT#: RC226083

WDR43 (Myc-DDK-tagged)-Human WD repeat domain 43 (WDR43)

Product Images

Circular map for RC226083

• 

  Western blot validation of overexpression lysate (Cat# LY429451) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC226083 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified WDR43 protein (Cat# TP326083). The protein was produced from HEK293T cells transfected with WDR43 cDNA clone (Cat# RC226083) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: WDR43
• Synonyms: NET12; UTP5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC226083 representing NM_015131
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCGGGCGGCGGCGGTAGCTGCGACCCCCTGGCCCCTGCTGGGGTCCCTTGCGCCTTCTCCCCGC
  ACAGCCAGGCCTACTTCGCTTTGGCCTCTACCGACGGTCACTTACGAGTATGGGAGACGGCCAACAACCG
  GCTGCACCAGGAGTACGTGCCTTCCGCGCACCTCAGTGGTACCTGCACCTGTCTGGCCTGGGCGCCAGCG
  CGGCTGCAGGCCAAGGAAAGTCCCCAGAGGAAAAAAAGGAAATCAGAAGCTGTAGGAATGAGTAACCAGA
  CTGACTTATTGGCTCTTGGCACAGCAGTTGGTAGCATTTTATTATACAGCACAGTAAAAGGAGAGTTACA
  CAGTAAATTAATAAGTGGTGGACATGACAACAGAGTCAACTGCATACAGTGGCATCAAGACAGTGGCTGT
  TTATATAGTTGTTCAGATGATAAACATATTGTGGAATGGAACGTACAGACATGCAAAGTAAAGTGCAAAT
  GGAAAGGCGACAATAGCAGTGTCAGTTCCCTATGTATCAGCCCAGATGGAAAGATGTTGCTTTCAGCTGG
  TCGAACAATCAAACTATGGGTTTTGGAGACCAAAGAAGTCTACAGGCATTTCACAGGACATGCAACGCCA
  GTTTCGTCACTGATGTTCACTACCATCAGACCTCCTAATGAGAGCCAGCCCTTTGATGGAATTACAGGTC
  TTTATTTCTTATCTGGAGCAGTACATGACCGGTTACTTAATGTCTGGCAGGTCCGATCAGAAAACAAAGA
  AAAGAGTGCAGTGATGTCATTTACAGTTACCGATGAACCTGTCTATATTGACTTAACTTTGTCAGAAAAC
  AAAGAAGAGCCTGTCAAGTTGGCTGTTGTTTGCAGAGATGGTCAAGTCCATCTTTTTGAACACATATTAA
  ATGGGTACTGCAAAAAGCCTTTGACTTCAAACTGCACAATTCAGATAGCAACACCTGGGAAAGGCAAGAA
  GTCAACACCAAAACCCATCCCTATTCTAGCTGCTGGTTTTTGCTCAGACAAAATGTCATTGTTGCTTGTA
  TATGGCAGTTGGTTTCAGCCTACTATTGAGCGAGTGGCTTTAAACTCCAGAGAACCTCATATGTGTTTAG
  TAAGAGATATTTCAAACTGCTGGGCCCCCAAAGTAGAAACAGCTATAACAAAGGTGAGGACACCAGTGAT
  GAATTCTGAAGCAAAAGTTCTGGTGCCTGGGATTCCTGGTCATCATGCAGCTATCAAGCCCGCTCCTCCA
  CAAACCGAGCAAGTAGAGAGCAAGAGGAAGTCAGGGGGAAATGAGGTTAGCATTGAAGAACGTCTGGGAG
  CAATGGATATAGACACACACAAAAAAGGAAAGGAAGACCTCCAGACGAATAGCTTTCCAGTTCTTCTTAC
  CCAGGGCTTAGAAAGTAACGATTTTGAAATGCTAAATAAAGTACTTCAAACTAGGAATGTAAACCTTATA
  AAGAAGACTGTATTAAGGATGCCCCTGCATACTATTATTCCGTTGTTACAAGAGCTTACAAAGAGGTTAC
  AAGGACATCCTAATAGTGCTGTGCTAATGGTTCAGTGGCTAAAATGTGTGTTAACAGTTCATGCATCATA
  CCTGTCCACGTTGCCTGACCTGGTACCCCAGCTGGGGACACTCTACCAGTTAATGGAAAGCAGAGTCAAA
  ACTTTTCAGAAACTTTCACACCTTCATGGAAAGCTTATTCTTCTAATTACACAAGTAACAGCATCAGAGA
  AGACAAAGGGAGCAACTTCCCCTGGACAGAAGGCAAAGTTGGTGTATGAAGAAGAGTCTTCTGAAGAGGA
  GTCTGATGATGAAATAGCAGATAAGGATTCTGAAGATAATTGGGATGAAGATGAGGAGGAGAGTGAAAGT
  GAAAAAGATGAGGACGTTGAAGAGGAAGATGAGGATGCCGAAGGAAAAGATGAAGAAAATGGCGAGGACA
  GAGATACAGCAAGTGAAAAAGAATTAAATGGAGATTCTGATTTAGATCCTGAAAATGAAAGTGAAGAAGA
  A
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC226083 representing NM_015131
  Red=Cloning site Green=Tags(s)
  
  MAAGGGGSCDPLAPAGVPCAFSPHSQAYFALASTDGHLRVWETANNRLHQEYVPSAHLSGTCTCLAWAPA
  RLQAKESPQRKKRKSEAVGMSNQTDLLALGTAVGSILLYSTVKGELHSKLISGGHDNRVNCIQWHQDSGC
  LYSCSDDKHIVEWNVQTCKVKCKWKGDNSSVSSLCISPDGKMLLSAGRTIKLWVLETKEVYRHFTGHATP
  VSSLMFTTIRPPNESQPFDGITGLYFLSGAVHDRLLNVWQVRSENKEKSAVMSFTVTDEPVYIDLTLSEN
  KEEPVKLAVVCRDGQVHLFEHILNGYCKKPLTSNCTIQIATPGKGKKSTPKPIPILAAGFCSDKMSLLLV
  YGSWFQPTIERVALNSREPHMCLVRDISNCWAPKVETAITKVRTPVMNSEAKVLVPGIPGHHAAIKPAPP
  QTEQVESKRKSGGNEVSIEERLGAMDIDTHKKGKEDLQTNSFPVLLTQGLESNDFEMLNKVLQTRNVNLI
  KKTVLRMPLHTIIPLLQELTKRLQGHPNSAVLMVQWLKCVLTVHASYLSTLPDLVPQLGTLYQLMESRVK
  TFQKLSHLHGKLILLITQVTASEKTKGATSPGQKAKLVYEEESSEEESDDEIADKDSEDNWDEDEEESES
  EKDEDVEEEDEDAEGKDEENGEDRDTASEKELNGDSDLDPENESEEE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_015131
• ORF Size: 2031 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015131.3
• RefSeq ORF: 2034 bp
• Locus ID: 23160
• UniProt ID: Q15061
• Cytogenetics: 2p23.2
• MW: 74.7 kDa
• Gene Summary: Ribosome biogenesis factor. Involved in nucleolar processing of pre-18S ribosomal RNA. Required for optimal pre-ribosomal RNA transcription by RNA polymerase I.[UniProtKB/Swiss-Prot Function]