CPEB2 (NM_182485) Human Tagged ORF Clone

CAT#: RC218561

CPEB2 (Myc-DDK-tagged)-Human cytoplasmic polyadenylation element binding protein 2 (CPEB2), transcript variant B

Product Images

Circular map for RC218561

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CPEB2
• Synonyms: CPE-BP2; CPEB-2; hCPEB-2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC218561 representing NM_182485
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGGATTTCGGGTTTGGGGTGCTGCAGACCGCCCCGCTCCGAAGTAGCAGTCCTGGGCCCCTGTTCT
  GCGGCGAGGCGTATGGTCCTTACGCCGTGGGGTCCGTCAACCCGCTGCCCTCCGCCACGCCCTTCGGCCC
  ACTGTCGCCACCACCGTTGCCTGTCACCGGCTTCTTAGAGGCCGCCTCCCCCTTCTCCGTCCCCCTCGGC
  GGCGGCGCGGGCAGCCCGGCCGCCGCCGCTTCCTCTTCCTCCCCGTTCCTGGCGCATCAGCAGACCATGC
  AGGATGAGCTGCTTCTGGGGCTGACACAGCAGCCGGCGCGGCCGCTTTCGGGGGCGGCGGCCACGGAGAA
  ACTCCCCGACCACCACCCCGGCGGCGGCACGATCGCGGGTGTGACCCACCTCCTCCCCTCCCAGGACTTC
  AAACCGAGTCTGCACCACCCCTCCTCCTCCTCCGCCTCCTCCTGCTGCTGCTGCCGCACCTCCTCCCCGC
  AGGACTTCAGTAAGCGGCAGCAGCAGCAGCTGAGCAGCCAGAAGAGGAAAGAGTTCAGCCCTCCCCACCT
  TCCCCACCCTCCGGACTCGAAGCCGCCGCCGCCGCCTCCGCCGCTCCACTGCCCCGGTCGGTTCAGCCCG
  CCGCCGCCGCCAGCCGGCCCGCTCCTCCAGCCGGCGCAGCTCGCTCAGCGCCAGCAGCAACAGCCGCCGC
  AGCAGTTCAGCCTCCTGCATCAGCAGCACCTCTCGCCGCAGGACTTCGCCCCGCGGCAGCGTCCGGCAGA
  CCTGCCCCCGCTCCCGCAGCTCCCTCCCTCGCCGCCTGCAGCCCCGCGGCGCCGCCACGGAGGCGCGGGC
  AGCCCTCGCAAGACCCCAGCCGCGGGCGAGGGCAGCGCCGCCGAGTCCCCCAATGCGGGCTTGGCCTCCT
  CGACGCCGGTGAACCCCGCGCCGGGCTCCATGGAGTCCCCCAACCACCCTCTGCTCAACAGTCCCAGTAA
  CCTCCTGCCCGGAGGTGCGCTTGGCGCGGGCGCCTTCAGCAGCCTGCAGAGCCCGGACCTTCCACACCCG
  GGCGGCGGCGGCGGCGGCGGGGGCGGGGGGCCCCCAGGAGGCGGAGGGGGAGGCGGCTCCGCGTCGCCGC
  CGCCGCTGCCCGGCTTCGGCACCCCCTGGTCGGTGCAGACCGCGTCGCCGCCACCCCAGCCCCAGCAGCC
  GCCGCCGACCCAGCCGCAGCAGCAGCCGCCGCCACCCCAGCAGCCGCCCCAGCCGCAGCCGCAGCCGCCC
  GGCTCGTCTGCCACCACCCCGGGCGGCGGCAGCGGCGGCTCGCTCAGCGCCATGCCGCCGCCCAGCCCCG
  ACTCAGAGAACGGCTTCTACCCCGGGCTGCCGTCGTCCATGAACCCGGCCTTCTTCCCTAGCTTCTCGCC
  CGTGTCGCCGCACGGCTGCACTGGGCTCAGCGTTCCGACGAGCGGCGGCGGCGGCGGCGGCTTCGGCGGC
  CCCTTCTCGGCTACCGCTGTGCCCCCTCCGCCGCCGCCCGCCATGAATATACCTCAACAGCAGCCCCCGC
  CGCCCGCGGCGCCGCAGCAGCCGCAGAGCCGGAGGTCGCCCGTCAGCCCGCAGCTCCAGCAGCAGCACCA
  GGCGGCGGCCGCCGCCTTCCTGCAGCAGAGGAACTCCTATAACCACCACCAGCCTCTTCTGAAACAGTCT
  CCCTGGAGCAACCATCAGAGCAGTGGCTGGGGCACTGGAAGTATGTCCTGGGGAGCAATGCATGGCAGAG
  ATCATCGTAGAACCGGAAACATGGGAATCCCAGGAACTATGAATCAGATATCTCCATTGAAGAAACCGTT
  TTCTGGTAATGTCATAGCACCACCGAAATTTACTCGCTCAACTCCATCACTGACTCCAAAATCTTGGATT
  GAAGATAATGTGTTCAGAACAGACAACAATAGTAATACACTCTTACCCTTACAGGTGAGATCTAGTTTGC
  AGTTGCCAGCTTGGGGCTCAGATTCACTCCAAGATAGTTGGTGCACTGCAGCCGGAACATCCAGAATAGA
  CCAGGATCGAAGTAGAATGTATGACAGTTTGAATATGCACTCTTTGGAAAATTCCCTTATCGATATTATG
  AGAGCAGAGCATGATCCTCTTAAGGGTCGATTGAGCTATCCACATCCAGGAACTGACAATCTGTTGATGT
  TAAATGGTCGTTCTTCCCTCTTTCCAATAGATGATGGCTTGCTTGATGATGGTCACAGTGATCAAGTTGG
  AGTTTTAAATTCACCCACCTGTTATTCAGCTCACCAAAATGGAGAGCGAATAGAACGCTTCTCTCGAAAA
  GTTTTTGTTGGTGGTCTTCCTCCAGATATTGATGAAGATGAAATAACTGCTAGCTTCAGAAGATTTGGGC
  CTTTGGTAGTAGATTGGCCTCATAAAGCAGAAAGCAAGTCCTATTTTCCACCAAAAGGCTATGCATTTCT
  TCTCTTTCAAGAAGAGAGCTCAGTTCAGGCACTCATTGATGCTTGTATTGAAGAAGATGGAAAACTCTAT
  CTGTGTGTTTCTAGCCCTACTATCAAGGACAAACCAGTTCAAATACGTCCTTGGAATTTAAGTGATAGTG
  ATTTTGTAATGGATGGTTCTCAGCCTTTGGATCCCCGAAAAACAATTTTTGTTGGAGGTGTTCCTAGGCC
  ATTAAGGGCTGTGGAACTTGCTATGATCATGGACCGGCTGTATGGTGGAGTTTGTTATGCAGGAATTGAT
  ACAGATCCTGAGCTAAAATACCCAAAAGGTGCTGGGCGAGTTGCTTTCTCCAATCAGCAGAGCTATATTG
  CTGCCATTAGTGCTCGGTTTGTTCAGCTTCAGCATGGTGATATTGATAAACGTGTGGAGGTAAAGCCATA
  TGTGCTAGATGACCAGATGTGTGATGAATGCCAGGGCGCACGCTGTGGTGGAAAATTTGCTCCCTTTTTT
  TGTGCCAATGTCACTTGCCTGCAGTATTACTGTGAGTTTTGTTGGGCAAATATCCACTCTCGTGCTGGAC
  GTGAGTTCCATAAGCCATTGGTAAAGGAAGGTGCTGATCGCCCACGTCAGATCCACTTCCGCTGGAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC218561 representing NM_182485
  Red=Cloning site Green=Tags(s)
  
  MRDFGFGVLQTAPLRSSSPGPLFCGEAYGPYAVGSVNPLPSATPFGPLSPPPLPVTGFLEAASPFSVPLG
  GGAGSPAAAASSSSPFLAHQQTMQDELLLGLTQQPARPLSGAAATEKLPDHHPGGGTIAGVTHLLPSQDF
  KPSLHHPSSSSASSCCCCRTSSPQDFSKRQQQQLSSQKRKEFSPPHLPHPPDSKPPPPPPPLHCPGRFSP
  PPPPAGPLLQPAQLAQRQQQQPPQQFSLLHQQHLSPQDFAPRQRPADLPPLPQLPPSPPAAPRRRHGGAG
  SPRKTPAAGEGSAAESPNAGLASSTPVNPAPGSMESPNHPLLNSPSNLLPGGALGAGAFSSLQSPDLPHP
  GGGGGGGGGGPPGGGGGGGSASPPPLPGFGTPWSVQTASPPPQPQQPPPTQPQQQPPPPQQPPQPQPQPP
  GSSATTPGGGSGGSLSAMPPPSPDSENGFYPGLPSSMNPAFFPSFSPVSPHGCTGLSVPTSGGGGGGFGG
  PFSATAVPPPPPPAMNIPQQQPPPPAAPQQPQSRRSPVSPQLQQQHQAAAAAFLQQRNSYNHHQPLLKQS
  PWSNHQSSGWGTGSMSWGAMHGRDHRRTGNMGIPGTMNQISPLKKPFSGNVIAPPKFTRSTPSLTPKSWI
  EDNVFRTDNNSNTLLPLQVRSSLQLPAWGSDSLQDSWCTAAGTSRIDQDRSRMYDSLNMHSLENSLIDIM
  RAEHDPLKGRLSYPHPGTDNLLMLNGRSSLFPIDDGLLDDGHSDQVGVLNSPTCYSAHQNGERIERFSRK
  VFVGGLPPDIDEDEITASFRRFGPLVVDWPHKAESKSYFPPKGYAFLLFQEESSVQALIDACIEEDGKLY
  LCVSSPTIKDKPVQIRPWNLSDSDFVMDGSQPLDPRKTIFVGGVPRPLRAVELAMIMDRLYGGVCYAGID
  TDPELKYPKGAGRVAFSNQQSYIAAISARFVQLQHGDIDKRVEVKPYVLDDQMCDECQGARCGGKFAPFF
  CANVTCLQYYCEFCWANIHSRAGREFHKPLVKEGADRPRQIHFRWN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_182485
• ORF Size: 3078 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_182485.3
• RefSeq Size: 6854 bp
• RefSeq ORF: 3081 bp
• Locus ID: 132864
• UniProt ID: Q7Z5Q1
• Cytogenetics: 4p15.32
• MW: 109.3 kDa
• Gene Summary: The protein encoded by this gene is highly similar to cytoplasmic polyadenylation element binding protein (CPEB), an mRNA-binding protein that regulates cytoplasmic polyadenylation of mRNA as a trans factor in oogenesis and spermatogenesis. Studies of the similar gene in mice suggested a possible role of this protein in transcriptionally inactive haploid spermatids. Alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2008]