FAM29A (HAUS6) (NM_017645) Human Tagged ORF Clone

CAT#: RC216553

HAUS6 (Myc-DDK-tagged)-Human HAUS augmin-like complex, subunit 6 (HAUS6)

Product Images

Circular map for RC216553

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  Western blot validation of overexpression lysate (Cat# LY413644) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC216553 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified HAUS6 protein (Cat# TP316553). The protein was produced from HEK293T cells transfected with HAUS6 cDNA clone (Cat# RC216553) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: FAM29A
• Synonyms: Dgt6; FAM29A
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC216553 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCTCGGCCTCGGTCACCGCTTTCGAGAAGGAGCATCTCTGGATGTATCTGCAGGCGCTCGGCTTCG
  AGCCAGGCCCGGCAACCATTGCCTGCGGAAAGATCGTGTCGCACACGCACCTCGGAGTGAACATGTTTGA
  CAAGCTGAACCGTGATGCCTTTCATATAATTTCTTATTTTTTGTTTCAAGTTCTGGACCAGTCTCTCACC
  AAAGAAGTTTTCAAATTTTGTTGGCCCCCATTTGACCAAAAAAGTGACACTGAATTCCGAAAACATTGCT
  GTGAATGGATAAAAAGGATTTCTGGTGAATGTGGAAGCAGCTTTCCTCAAGTTGTTGGTTCACTATTTCT
  TTCTCCTGGTGGTCCTAAGTTTATTCATCTGATGTATCATTTTGCAAGATTTGTTGCAATGAAATATATT
  AAATCCAATTCTAAAAATTCTTCTCATCATTTTGTAGAGACATTTAACATAAAACCACAGGACTTGCACA
  AATGCATTGCCAGATGCCATTTCGCACGTAGCAGATTTTTACAAATTTTGCAAAGACAAGATTGTGTTAC
  CCAAAAATATCAGGAAAATGCACAATTATCAGTTAAGCAGGTACGAAACTTGAGATCTGAATGTATAGGA
  TTGGAAAACCAAATAAAGAAAATGGAACCCTATGATGACCACAGTAATATGGAAGAAAAAATTCAAAAGG
  TTCGGTCTTTGTGGGCTTCAGTGAATGAAACGCTCATGTTTTTGGAAAAAGAGAGAGAAGTTGTTAGTTC
  GGTCCTTAGTCTTGTTAACCAATATGCTTTAGATGGAACTAATGTTGCTATTAATATTCCAAGGCTCTTA
  CTTGACAAAATTGAGAAACAAATGTTTCAGTTGCACATAGGAAATGTTTATGAGGCTGGAAAACTGAACC
  TCTTAACAGTTATTCAGTTATTAAATGAAGTCTTGAAGGTGATGAAATATGAACGTTGTCAGGCTGATCA
  AGCAAGATTGACGGTAGACCTTCACTACCTTGAAAAAGAGACCAAATTTCAGAAGGAAAGATTATCAGAT
  CTGAAACATATGAGGTATAGAATAAAAGATGATCTCACAACTATAAGACATTCTGTTGTTGAAAAGCAAG
  GAGAATGGCATAAAAAGTGGAAAGAATTTCTTGGTTTGTCTCCTTTCAGTCTAATTAAAGGTTGGACTCC
  ATCTGTAGATCTTTTACCACCAATGTCTCCCCTTTCGTTTGATCCTGCCTCAGAAGAAGTGTATGCAAAG
  AGTATTCTTTGTCAGTATCCTGCTTCACTTCCAGATGCACATAAGCAACATAACCAAGAAAATGGTTGCA
  GAGGAGACAGTGATACCTTGGGAGCGCTACATGATCTAGCCAACAGCCCTGCCTCTTTCTTGTCGCAGTC
  AGTTTCATCATCAGATAGAAACAGTGTTACAGTACTTGAAAAGGACACAAAGATGGGAACTCCCAAAGAA
  AAAAATGAAGCAATTTCTAAGAAAATACCAGAATTTGAAGTGGAAAATTCTCCATTATCAGATGTTGCAA
  AGAATACAGAGAGTAGTGCATTTGGAGGGTCTTTGCCAGCTAAAAAAAGTGATCCATTTCAAAAAGAGCA
  AGATCATCTGGTAGAAGAGGTTGCCAGAGCAGTTTTATCTGATTCACCACAGCTCTCTGAAGGAAAAGAA
  ATAAAATTAGAGGAACTAATTGACTCTCTGGGTTCTAACCCCTTCTTAACAAGGAATCAGATTCCCCGTA
  CTCCAGAAAACTTGATAACTGAAATTAGGAGCTCATGGAGAAAAGCTATTGAAATGGAAGAAAACAGAAC
  TAAAGAACCAATTCAAATGGATGTTGAACATAGAGAAGTATTGCCAGAATCATTACCTGTGTTGCACAAT
  CAAAGAGAATTTAGCATGGCTGATTTTCTCTTAGAAACCACTGTATCAGATTTTGGCCAGTCTCATTTGA
  CTGAAGAGAAAGTTATTTCAGATTGCGAGTGTGTGCCTCAGAAACATGTGCTGACCAGTCACATAGATGA
  ACCACCAACACAAAATCAGTCAGATTTGTTAAATAAGAAAGTAATTTGCAAGCAAGATTTGGAATGTTTA
  GCCTTCACCAAGCTTTCAGAAACTAGCCGAATGGAGACATTCTCCCCTGCTGTCGGCAATAGGATAGATG
  TGATGGGTGGCAGTGAAGAGGAGTTTATGAAAATATTGGACCACTTAGAAGTTTCTTGTAACAAACCTTC
  CACAAATAAAACTATGTTGTGGAATTCTTTTCAGATATCAATTGGAATTAGTTCTAAGAGTTTTAAAGAT
  AATGATTTTGGCATATTACACGAAACTCTCCCGGAAGAAGTTGGTCATCTAAGTTTTAATAGTTCCAGTA
  GTTCAGAGGCCAATTTTAAACTGGAGCCAAATAGTCCTATGCATGGTGGCACTCTTCTAGAAGATGTTGT
  GGGAGGGAGACAGACTACTCCAGAATCAGACTTTAATTTACAGGCTCTTCGCAGTAGATACGAGGCTCTG
  AAGAAATCTCTTTCCAAGAAAAGGGAAGAATCTTACCTCTCGAATTCCCAAACACCCGAAAGACACAAAC
  CAGAATTGAGCCCTACTCCCCAAAATGTACAAACAGATGATACGCTTAACTTTTTGGACACCTGTGATTT
  GCATACTGAGCATATAAAGCCATCTTTACGCACGTCCATCGGTGAAAGAAAACGGTCTCTTTCACCACTA
  ATTAAGTTTTCTCCAGTGGAACAAAGATTGAGAACCACAATAGCATGTAGTCTTGGAGAACTACCTAATT
  TAAAGGAAGAAGACATTTTGAATAAGAGCCTTGATGCAAAAGAACCACCGTCTGACTTGACAAGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC216553 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSSASVTAFEKEHLWMYLQALGFEPGPATIACGKIVSHTHLGVNMFDKLNRDAFHIISYFLFQVLDQSLT
  KEVFKFCWPPFDQKSDTEFRKHCCEWIKRISGECGSSFPQVVGSLFLSPGGPKFIHLMYHFARFVAMKYI
  KSNSKNSSHHFVETFNIKPQDLHKCIARCHFARSRFLQILQRQDCVTQKYQENAQLSVKQVRNLRSECIG
  LENQIKKMEPYDDHSNMEEKIQKVRSLWASVNETLMFLEKEREVVSSVLSLVNQYALDGTNVAINIPRLL
  LDKIEKQMFQLHIGNVYEAGKLNLLTVIQLLNEVLKVMKYERCQADQARLTVDLHYLEKETKFQKERLSD
  LKHMRYRIKDDLTTIRHSVVEKQGEWHKKWKEFLGLSPFSLIKGWTPSVDLLPPMSPLSFDPASEEVYAK
  SILCQYPASLPDAHKQHNQENGCRGDSDTLGALHDLANSPASFLSQSVSSSDRNSVTVLEKDTKMGTPKE
  KNEAISKKIPEFEVENSPLSDVAKNTESSAFGGSLPAKKSDPFQKEQDHLVEEVARAVLSDSPQLSEGKE
  IKLEELIDSLGSNPFLTRNQIPRTPENLITEIRSSWRKAIEMEENRTKEPIQMDVEHREVLPESLPVLHN
  QREFSMADFLLETTVSDFGQSHLTEEKVISDCECVPQKHVLTSHIDEPPTQNQSDLLNKKVICKQDLECL
  AFTKLSETSRMETFSPAVGNRIDVMGGSEEEFMKILDHLEVSCNKPSTNKTMLWNSFQISIGISSKSFKD
  NDFGILHETLPEEVGHLSFNSSSSSEANFKLEPNSPMHGGTLLEDVVGGRQTTPESDFNLQALRSRYEAL
  KKSLSKKREESYLSNSQTPERHKPELSPTPQNVQTDDTLNFLDTCDLHTEHIKPSLRTSIGERKRSLSPL
  IKFSPVEQRLRTTIACSLGELPNLKEEDILNKSLDAKEPPSDLTR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_017645
• ORF Size: 2865 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_017645.5
• RefSeq Size: 6365 bp
• RefSeq ORF: 2868 bp
• Locus ID: 54801
• UniProt ID: Q7Z4H7
• Cytogenetics: 9p22.1
• MW: 108.7 kDa
• Gene Summary: The protein encoded by this gene is a subunit of the augmin complex. The augmin complex plays a role in microtubule attachment to the kinetochore and central spindle formation. This protein may have a role in efficient chromosome congression and segregation by promoting microtubule-dependent microtubule amplification. Pseudogenes of this gene are located on chromosomes 7 and 20. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, Aug 2012]