KIF3C (NM_002254) Human Tagged ORF Clone

CAT#: RC216137

KIF3C (Myc-DDK-tagged)-Human kinesin family member 3C (KIF3C)

Product Images

Circular map for RC216137

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  Western blot validation of overexpression lysate (Cat# LY419419) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC216137 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified KIF3C protein (Cat# TP316137). The protein was produced from HEK293T cells transfected with KIF3C cDNA clone (Cat# RC216137) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: KIF3C
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC216137 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCAGTAAGACCAAGGCCAGCGAGGCCCTCAAGGTGGTGGCCCGGTGCCGCCCCCTCAGCAGGAAGG
  AGGAGGCTGCTGGTCACGAGCAGATCCTGACCATGGACGTGAAACTGGGCCAGGTGACCCTGCGGAACCC
  CCGCGCTGCCCCGGGGGAGCTGCCCAAGACCTTCACCTTTGACGCCGTGTATGATGCCAGCTCCAAGCAG
  GCCGACCTGTATGACGAAACCGTGAGGCCCCTGATAGACTCCGTGCTCCAGGGTTTCAATGGCACGGTGT
  TTGCCTATGGCCAGACGGGCACTGGCAAGACCTATACCATGCAGGGGACCTGGGTGGAGCCCGAGCTGCG
  CGGGGTCATCCCGAATGCCTTTGAGCACATCTTCACCCACATCTCCCGCTCCCAGAACCAACAGTACCTG
  GTCCGGGCCTCCTATTTGGAGATCTACCAGGAAGAGATTCGAGACCTGCTCTCCAAGGAGCCGGGCAAGA
  GGCTAGAGCTGAAAGAGAACCCCGAGACTGGCGTCTACATCAAGGACCTCTCCTCCTTCGTCACCAAGAA
  TGTCAAGGAGATTGAGCATGTGATGAACCTGGGGAACCAGACCCGGGCTGTGGGCAGCACCCACATGAAT
  GAGGTCAGCTCCCGCTCCCATGCCATCTTCATCATCACTGTGGAGTGCAGCGAACGTGGCTCTGATGGCC
  AGGACCACATCCGAGTGGGCAAGCTCAACCTCGTGGACCTGGCTGGCAGCGAGAGGCAGAACAAGGCAGG
  CCCCAACACAGCGGGAGGGGCAGCCACACCATCCTCGGGTGGCGGTGGTGGCGGTGGAGGCAGTGGTGGT
  GGTGCTGGTGGAGAGAGGCCTAAGGAAGCCTCCAAAATCAACCTCTCATTATCTGCCCTGGGCAACGTGA
  TTGCTGCCCTGGCGGGCAACAGGAGCACCCACATTCCCTACCGGGACTCCAAGCTGACCCGGCTGCTCCA
  GGACTCCCTGGGGGGGAATGCCAAGACCATCATGGTAGCCACACTGGGGCCAGCTTCTCACAGCTACGAT
  GAGAGCCTCTCCACCTTGCGCTTTGCCAACCGAGCCAAGAACATCAAGAACAAGCCCCGGGTGAACGAGG
  ACCCCAAGGACACACTGCTGCGGGAATTCCAAGAGGAGATTGCCCGCCTGAAGGCCCAGCTGGAGAAGAG
  GGGGATGCTGGGGAAGCGGCCCCGGAGGAAGAGCAGCCGCAGGAAGAAGGCCGTGTCCGCCCCGCCTGGG
  TACCCTGAGGGCCCAGTGATTGAGGCCTGGGTGGCAGAAGAGGAGGATGACAACAACAACAACCACCGCC
  CGCCCCAGCCCATCCTGGAGTCAGCCTTGGAGAAGAACATGGAGAATTACCTGCAGGAACAGAAGGAGCG
  GCTGGAGGAGGAGAAGGCAGCCATCCAGGATGACCGCAGCCTGGTGAGCGAGGAGAAGCAGAAGCTGCTG
  GAGGAGAAGGAGAAGATGCTGGAGGACCTGCGGCGGGAACAGCAGGCCACAGAGCTGCTTGCGGCCAAGT
  ACAAGGCCATGGAGAGCAAGCTCCTCATCGGGGGCAGGAACATCATGGATCACACCAACGAACAGCAGAA
  GATGTTGGAACTGAAGAGGCAGGAGATTGCCGAGCAGAAACGTCGTGAGCGGGAGATGCAGCAGGAGATG
  ATGCTCCGGGACGAGGAGACTATGGAGCTCCGGGGCACCTACACATCCCTGCAGCAGGAGGTGGAGGTCA
  AAACCAAGAAACTCAAGAAGCTCTACGCCAAGCTGCAGGCGGTGAAGGCGGAGATCCAGGACCAGCATGA
  TGAGTACATCCGCGTGCGGCAGGACCTGGAGGAGGCGCAGAACGAGCAGACCCGCGAACTCAAGCTCAAG
  TACCTAATCATCGAGAACTTCATCCCGCCGGAGGAGAAGAACAAGATCATGAACCGGCTTTTCCTGGACT
  GTGAGGAGGAGCAGTGGAAGTTCCAGCCACTGGTGCCAGCCGGCGTCAGTAGCAGCCAGATGAAGAAGCG
  GCCAACATCTGCAGTGGGCTACAAGAGGCCTATCAGCCAGTATGCTCGGGTTGCCATGGCAATGGGGTCC
  CACCCCAGGTACAGGGCTGAAAACATAATGTTTCTGGAGTTGGATGTGTCCCCTCCAGCTGTCTTTGAGA
  TGGAATTCTCTCACGACCAAGAACAAGACCCTCGTGCGCTACACATGGAGAGGCTCATGCGATTGGACAG
  CTTTCTGGAAAGACCTTCCACGTCTAAAGTCCGAAAGTCCAGATCCTGGTGCCAGAGTCCTCAGCGGCCT
  CCACCTTCCACCACACATGCCTCCCTGGCCTCTGCTTCTCTGCGCCCTGCAACAGTGGCGGACCATGAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC216137 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MASKTKASEALKVVARCRPLSRKEEAAGHEQILTMDVKLGQVTLRNPRAAPGELPKTFTFDAVYDASSKQ
  ADLYDETVRPLIDSVLQGFNGTVFAYGQTGTGKTYTMQGTWVEPELRGVIPNAFEHIFTHISRSQNQQYL
  VRASYLEIYQEEIRDLLSKEPGKRLELKENPETGVYIKDLSSFVTKNVKEIEHVMNLGNQTRAVGSTHMN
  EVSSRSHAIFIITVECSERGSDGQDHIRVGKLNLVDLAGSERQNKAGPNTAGGAATPSSGGGGGGGGSGG
  GAGGERPKEASKINLSLSALGNVIAALAGNRSTHIPYRDSKLTRLLQDSLGGNAKTIMVATLGPASHSYD
  ESLSTLRFANRAKNIKNKPRVNEDPKDTLLREFQEEIARLKAQLEKRGMLGKRPRRKSSRRKKAVSAPPG
  YPEGPVIEAWVAEEEDDNNNNHRPPQPILESALEKNMENYLQEQKERLEEEKAAIQDDRSLVSEEKQKLL
  EEKEKMLEDLRREQQATELLAAKYKAMESKLLIGGRNIMDHTNEQQKMLELKRQEIAEQKRREREMQQEM
  MLRDEETMELRGTYTSLQQEVEVKTKKLKKLYAKLQAVKAEIQDQHDEYIRVRQDLEEAQNEQTRELKLK
  YLIIENFIPPEEKNKIMNRLFLDCEEEQWKFQPLVPAGVSSSQMKKRPTSAVGYKRPISQYARVAMAMGS
  HPRYRAENIMFLELDVSPPAVFEMEFSHDQEQDPRALHMERLMRLDSFLERPSTSKVRKSRSWCQSPQRP
  PPSTTHASLASASLRPATVADHE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_002254
• ORF Size: 2379 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002254.8
• RefSeq Size: 5431 bp
• RefSeq ORF: 2382 bp
• Locus ID: 3797
• UniProt ID: O14782
• Cytogenetics: 2p23.3
• Domains: kinesin
• Protein Families: Druggable Genome
• MW: 89.5 kDa
• Gene Summary: Microtubule-based anterograde translocator for membranous organelles.[UniProtKB/Swiss-Prot Function]