IRE1 (ERN1) (NM_001433) Human Tagged ORF Clone

CAT#: RC215023

ERN1 (Myc-DDK-tagged)-Human endoplasmic reticulum to nucleus signaling 1 (ERN1)

Product Images

Circular map for RC215023

• 

  Western blot validation of overexpression lysate (Cat# LY419940) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC215023 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified ERN1 protein (Cat# TP315023). The protein was produced from HEK293T cells transfected with ERN1 cDNA clone (Cat# RC215023) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: IRE1
• Synonyms: hIRE1p; IRE1; IRE1a; IRE1P
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC215023 representing NM_001433
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGGCCCGGCGGCTGCTGCTGCTGCTGACGCTGCTGCTGCCCGGCCTCGGGATTTTTGGAAGTACCA
  GCACAGTGACGCTTCCTGAAACCTTGTTGTTTGTGTCAACGCTGGATGGAAGTTTGCATGCTGTCAGCAA
  GAGGACAGGCTCAATCAAATGGACTTTAAAAGAAGATCCAGTCCTGCAGGTCCCAACACATGTGGAAGAG
  CCTGCCTTTCTCCCAGATCCTAATGATGGCAGCCTGTATACGCTTGGAAGCAAGAATAATGAAGGCCTGA
  CGAAACTTCCTTTTACCATCCCAGAATTGGTGCAGGCATCCCCATGCCGAAGTTCAGATGGAATCCTCTA
  CATGGGTAAAAAGCAGGACATCTGGTATGTTATTGACCTCCTGACCGGAGAGAAGCAGCAGACTTTGTCA
  TCGGCCTTTGCAGATAGTCTCTGCCCATCAACCTCTCTTCTGTATCTTGGGCGAACAGAATACACCATCA
  CCATGTACGACACCAAAACCCGAGAGCTCCGGTGGAATGCCACCTACTTTGACTATGCGGCCTCACTGCC
  TGAGGACGACGTGGACTACAAGATGTCCCACTTTGTGTCCAATGGTGATGGGCTGGTGGTGACTGTGGAC
  AGTGAATCTGGGGACGTCCTATGGATCCAAAACTACGCCTCCCCTGTGGTGGCCTTTTATGTCTGGCAGC
  GGGAGGGTCTGAGGAAGGTGATGCACATCAATGTCGCTGTGGAGACCCTGCGCTATCTGACCTTCATGTC
  TGGGGAGGTGGGGCGCATCACAAAGTGGAAGTACCCGTTCCCCAAGGAGACAGAGGCCAAGAGCAAGCTG
  ACGCCCACTCTGTATGTTGGGAAATACTCTACCAGCCTCTATGCCTCTCCCTCAATGGTACACGAGGGGG
  TTGCTGTCGTGCCCCGCGGCAGCACACTTCCTTTGCTGGAAGGGCCCCAGACTGATGGCGTCACCATCGG
  GGACAAGGGGGAGTGTGTGATCACGCCCAGCACGGACGTCAAGTTTGATCCCGGACTCAAAAGCAAGAAC
  AAGCTCAACTACTTGAGGAATTACTGGCTTCTGATAGGACACCATGAAACCCCACTGTCTGCGTCTACCA
  AGATGCTGGAGAGATTTCCCAACAATCTACCCAAACATCGGGAAAATGTGATTCCTGCTGATTCAGAGAA
  AAAGAGCTTTGAGGAAGTTATCAACCTGGTTGACCAGACTTCAGAAAACGCACCTACCACCGTGTCTCGG
  GATGTGGAGGAGAAGCCCGCCCATGCCCCTGCCCGGCCCGAGGCCCCCGTGGACTCCATGCTTAAGGACA
  TGGCTACCATCATCCTGAGCACCTTCCTGCTGATTGGCTGGGTGGCCTTCATCATCACCTATCCCCTGAG
  CATGCATCAGCAGCAGCAGCTCCAGCACCAGCAGTTCCAGAAGGAACTGGAGAAGATCCAGCTCCTGCAG
  CAGCAGCAGCAGCAGCTGCCCTTCCACCCACCTGGAGACACGGCTCAGGACGGCGAGCTCCTGGACACGT
  CTGGCCCGTACTCAGAGAGCTCGGGCACCAGCAGCCCCAGCACGTCCCCCAGGGCCTCCAACCACTCGCT
  CTGCTCCGGCAGCTCTGCCTCCAAGGCTGGCAGCAGCCCCTCCCTGGAACAAGACGATGGAGATGAGGAA
  ACCAGCGTGGTGATAGTTGGGAAAATTTCCTTCTGTCCCAAGGATGTCCTGGGCCATGGAGCTGAGGGCA
  CAATTGTGTACCGGGGCATGTTTGACAACCGCGACGTGGCCGTGAAGAGGATCCTCCCCGAGTGTTTTAG
  CTTCGCAGACCGTGAGGTCCAGCTGTTGCGAGAATCGGATGAGCACCCGAACGTGATCCGCTACTTCTGC
  ACGGAGAAGGACCGGCAATTCCAGTACATTGCCATCGAGCTGTGTGCAGCCACCCTGCAAGAGTATGTGG
  AGCAGAAGGACTTTGCGCATCTCGGCCTGGAGCCCATCACCTTGCTGCAGCAGACCACCTCGGGCCTGGC
  CCACCTCCACTCCCTCAACATCGTTCACAGAGACCTAAAGCCACACAACATCCTCATATCCATGCCCAAT
  GCACACGGCAAGATCAAGGCCATGATCTCCGACTTTGGCCTCTGCAAGAAGCTGGCAGTGGGCAGACACA
  GTTTCAGCCGCCGATCTGGGGTGCCTGGCACAGAAGGCTGGATCGCTCCAGAGATGCTGAGCGAAGACTG
  TAAGGAGAACCCTACCTACACGGTGGACATCTTTTCTGCAGGCTGCGTCTTTTACTACGTAATCTCTGAG
  GGCAGCCACCCTTTTGGCAAGTCCCTGCAGCGGCAGGCCAACATCCTCCTGGGTGCCTGCAGCCTTGACT
  GCTTGCACCCAGAGAAGCACGAAGACGTCATTGCACGTGAATTGATAGAGAAGATGATTGCGATGGATCC
  TCAGAAACGCCCCTCAGCGAAGCACGTGCTCAAACACCCGTTCTTCTGGAGCCTAGAGAAGCAGCTCCAG
  TTCTTCCAGGACGTGAGCGACAGAATAGAAAAGGAATCCCTGGATGGCCCGATCGTGAAGCAGTTAGAGA
  GAGGCGGGAGAGCCGTGGTGAAGATGGACTGGCGGGAGAACATCACTGTCCCCCTCCAGACAGACCTGCG
  TAAATTCAGGACCTATAAAGGTGGTTCTGTCAGAGATCTCCTCCGAGCCATGAGAAATAAGAAGCACCAC
  TACCGGGAGCTGCCTGCAGAGGTGCGGGAGACGCTGGGGTCCCTCCCCGACGACTTCGTGTGCTACTTCA
  CATCTCGCTTCCCCCACCTCCTCGCACACACCTACCGGGCCATGGAGCTGTGCAGCCACGAGAGACTCTT
  CCAGCCCTACTACTTCCACGAGCCCCCAGAGCCCCAGCCCCCAGTGACTCCAGACGCCCTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC215023 representing NM_001433
  Red=Cloning site Green=Tags(s)
  
  MPARRLLLLLTLLLPGLGIFGSTSTVTLPETLLFVSTLDGSLHAVSKRTGSIKWTLKEDPVLQVPTHVEE
  PAFLPDPNDGSLYTLGSKNNEGLTKLPFTIPELVQASPCRSSDGILYMGKKQDIWYVIDLLTGEKQQTLS
  SAFADSLCPSTSLLYLGRTEYTITMYDTKTRELRWNATYFDYAASLPEDDVDYKMSHFVSNGDGLVVTVD
  SESGDVLWIQNYASPVVAFYVWQREGLRKVMHINVAVETLRYLTFMSGEVGRITKWKYPFPKETEAKSKL
  TPTLYVGKYSTSLYASPSMVHEGVAVVPRGSTLPLLEGPQTDGVTIGDKGECVITPSTDVKFDPGLKSKN
  KLNYLRNYWLLIGHHETPLSASTKMLERFPNNLPKHRENVIPADSEKKSFEEVINLVDQTSENAPTTVSR
  DVEEKPAHAPARPEAPVDSMLKDMATIILSTFLLIGWVAFIITYPLSMHQQQQLQHQQFQKELEKIQLLQ
  QQQQQLPFHPPGDTAQDGELLDTSGPYSESSGTSSPSTSPRASNHSLCSGSSASKAGSSPSLEQDDGDEE
  TSVVIVGKISFCPKDVLGHGAEGTIVYRGMFDNRDVAVKRILPECFSFADREVQLLRESDEHPNVIRYFC
  TEKDRQFQYIAIELCAATLQEYVEQKDFAHLGLEPITLLQQTTSGLAHLHSLNIVHRDLKPHNILISMPN
  AHGKIKAMISDFGLCKKLAVGRHSFSRRSGVPGTEGWIAPEMLSEDCKENPTYTVDIFSAGCVFYYVISE
  GSHPFGKSLQRQANILLGACSLDCLHPEKHEDVIARELIEKMIAMDPQKRPSAKHVLKHPFFWSLEKQLQ
  FFQDVSDRIEKESLDGPIVKQLERGGRAVVKMDWRENITVPLQTDLRKFRTYKGGSVRDLLRAMRNKKHH
  YRELPAEVRETLGSLPDDFVCYFTSRFPHLLAHTYRAMELCSHERLFQPYYFHEPPEPQPPVTPDAL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001433
• ORF Size: 2931 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001433.5
• RefSeq Size: 3620 bp
• RefSeq ORF: 2934 bp
• Locus ID: 2081
• UniProt ID: O75460
• Cytogenetics: 17q23.3
• Domains: pkinase, TyrKc, S_TKc, PQQ, PUG
• Protein Families: Protein Kinase, Transmembrane
• Protein Pathways: Alzheimer's disease
• MW: 109.6 kDa
• Gene Summary: This gene encodes the transmembrane protein kinase inositol-requiring enzyme 1. The encoded protein contains two functional catalytic domains, a serine/threonine-protein kinase domain and an endoribonuclease domain. This protein functions as a sensor of unfolded proteins in the endoplasmic reticulum (ER) and triggers an intracellular signaling pathway termed the unfolded protein response (UPR). The UPR is an ER stress response that is conserved from yeast to mammals and activates genes involved in degrading misfolded proteins, regulating protein synthesis and activating molecular chaperones. This protein specifically mediates the splicing and activation of the stress response transcription factor X-box binding protein 1. [provided by RefSeq, Aug 2017]