WDR55 (NM_017706) Human Tagged ORF Clone

CAT#: RC209753

WDR55 (Myc-DDK-tagged)-Human WD repeat domain 55 (WDR55)

Product Images

Circular map for RC209753

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  Western blot validation of overexpression lysate (Cat# LY413614) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC209753 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified WDR55 protein (Cat# TP309753). The protein was produced from HEK293T cells transfected with WDR55 cDNA clone (Cat# RC209753) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: WDR55
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC209753 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACCGCACTTGTGAGGAGAGGCCCGCTGAGGATGGGAGCGACGAGGAGGACCCAGACTCCATGGAAG
  CCCCAACCCGGATCCGGGACACTCCGGAAGACATCGTGCTGGAAGCTCCGGCTAGTGGGCTGGCGTTCCA
  TCCGGCCCGTGACCTACTGGCTGCAGGGGACGTGGACGGGGACGTGTTCGTCTTTTCCTACTCTTGCCAA
  GAGGGAGAAACCAAGGAGCTCTGGTCATCAGGTCACCATCTCAAGGCCTGCCGAGCTGTGGCCTTCTCTG
  AAGATGGGCAGAAGCTCATTACTGTCTCCAAGGACAAAGCCATCCATGTTCTAGATGTGGAGCAGGGCCA
  ACTGGAAAGACGTGTTTCCAAGGCTCATGGTGCCCCCATCAACAGTCTTCTGCTGGTGGATGAGAATGTT
  CTGGCCACTGGGGATGACACAGGTGGTATCCGTCTCTGGGACCAGCGGAAGGAGGGCCCCTTAATGGATA
  TGAGGCAACATGAAGAGTACATCGCAGACATGGCTCTGGATCCAGCCAAAAAGCTGCTGCTGACAGCCAG
  CGGGGATGGCTGCCTTGGCATCTTCAACATTAAGAGGCGTCGGTTTGAGCTGCTCTCAGAACCTCAGTCT
  GGGGACCTGACCTCTGTCACTCTCATGAAATGGGGGAAGAAGGTAGCCTGTGGCTCCAGTGAAGGTACCA
  TCTACCTCTTCAATTGGAATGGCTTTGGGGCCACAAGTGACCGCTTTGCCCTGAGAGCTGAATCTATCGA
  CTGCATGGTTCCAGTCACCGAGAGTCTGCTGTGTACTGGCTCCACTGATGGAGTCATCAGGGCTGTGAAC
  ATCCTACCGAACCGAGTGGTGGGCAGTGTGGGCCAGCACACTGGGGAGCCTGTGGAGGAGCTGGCCCTCT
  CCCACTGTGGCCGCTTCCTGGCCAGTAGTGGCCATGACCAGCGCCTCAAGTTTTGGGACATGGCCCAGCT
  GCGAGCTGTGGTGGTGGATGACTACCGTCGGCGCAAAAAAAAGGGAGGACCACTGCGGGCTCTGAGCAGC
  AAGACTTGGAGCACCGATGACTTCTTCGCAGGACTGAGGGAAGAGGGAGAAGACTCCATGGCTCAGGAAG
  AAAAGGAGGAGACTGGGGATGACAGTGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC209753 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MDRTCEERPAEDGSDEEDPDSMEAPTRIRDTPEDIVLEAPASGLAFHPARDLLAAGDVDGDVFVFSYSCQ
  EGETKELWSSGHHLKACRAVAFSEDGQKLITVSKDKAIHVLDVEQGQLERRVSKAHGAPINSLLLVDENV
  LATGDDTGGIRLWDQRKEGPLMDMRQHEEYIADMALDPAKKLLLTASGDGCLGIFNIKRRRFELLSEPQS
  GDLTSVTLMKWGKKVACGSSEGTIYLFNWNGFGATSDRFALRAESIDCMVPVTESLLCTGSTDGVIRAVN
  ILPNRVVGSVGQHTGEPVEELALSHCGRFLASSGHDQRLKFWDMAQLRAVVVDDYRRRKKKGGPLRALSS
  KTWSTDDFFAGLREEGEDSMAQEEKEETGDDSD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_017706
• ORF Size: 1149 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_017706.4
• RefSeq Size: 2580 bp
• RefSeq ORF: 1152 bp
• Locus ID: 54853
• UniProt ID: Q9H6Y2
• Cytogenetics: 5q31.3
• Domains: WD40
• MW: 42.1 kDa
• Gene Summary: Nucleolar protein that acts as a modulator of rRNA synthesis. Plays a central role during organogenesis (By similarity).[UniProtKB/Swiss-Prot Function]