EXOC3 (NM_007277) Human Tagged ORF Clone

CAT#: RC209413

EXOC3 (Myc-DDK-tagged)-Human exocyst complex component 3 (EXOC3)

Product Images

Circular map for RC209413

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  Western blot validation of overexpression lysate (Cat# LY402127) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC209413 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified EXOC3 protein (Cat# TP309413). The protein was produced from HEK293T cells transfected with EXOC3 cDNA clone (Cat# RC209413) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: EXOC3
• Synonyms: SEC6; SEC6L1; Sec6p
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC209413 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGGAGACAGACCGGGAGGCCGTTGCGACAGCAGTGCAAAGGGTTGCTGGGATGCTCCAGCGCCCGG
  ACCAGCTGGACAAGGTGGAGCAGTATCGCAGGAGAGAAGCGCGGAAGAAGGCCTCCGTGGAGGCCAGATT
  GAAGGCCGCCATCCAGTCACAGTTGGACGGGGTGCGCACAGGCCTCAGCCAGCTCCACAACGCCCTGAAT
  GACGTCAAAGACATCCAGCAGTCGCTGGCAGACGTCAGCAAGGACTGGAGGCAGAGCATCAACACCATTG
  AGAGCCTCAAGGACGTCAAAGACGCCGTGGTGCAGCACAGCCAGCTCGCCGCAGCCGTGGAGAACCTCAA
  GAACATCTTCTCAGTGCCTGAGATTGTGAGGGAGACCCAGGACCTAATTGAACAAGGGGCACTCCTGCAA
  GCCCACCGGAAGCTGATGGACCTGGAATGCTCCCGGGACGGGCTGATGTACGAGCAGTACCGCATGGACA
  GTGGGAACACGCGTGACATGACCCTCATCCATGGCTACTTTGGCAGCACGCAGGGGCTCTCTGATGAGCT
  GGCTAAGCAGCTGTGGATGGTGCTGCAGAGGTCACTGGTCACTGTCCGCCGTGACCCCACCTTGCTGGTC
  TCAGTTGTCAGGATCATTGAAAGGGAAGAGAAAATTGACAGGCGCATACTTGACCGGAAAAAGCAAACTG
  GCTTTGTTCCTCCTGGGAGGCCCAAGAATTGGAAGGAGAAAATGTTCACCATCTTGGAGAGGACTGTGAC
  CACCAGAATTGAGGGCACACAGGCAGATACCAGAGAGTCTGACAAGATGTGGCTTGTCCGCCACCTGGAA
  ATTATAAGGAAGTACGTCCTGGATGACCTCATTGTCGCCAAAAACCTGATGGTTCAGTGCTTTCCTCCCC
  ACTATGAGATCTTTAAGAACCTCCTGAACATGTACCACCAAGCCCTGAGCACGCGGATGCAGGACCTCGC
  ATCGGAAGACCTGGAAGCCAATGAGATCGTGAGCCTCTTGACGTGGGTCTTAAACACCTACACAAGTACT
  GAGATGATGAGGAACGTGGAGCTGGCCCCGGAAGTGGATGTCGGCACCCTGGAGCCATTGCTTTCTCCAC
  ACGTGGTCTCTGAGCTGCTTGACACGTACATGTCCACGCTCACTTCAAACATCATCGCCTGGCTGCGGAA
  AGCGCTGGAGACAGACAAGAAAGACTGGGTCAAAGAGACAGAGCCAGAAGCCGACCAGGACGGGTACTAC
  CAGACCACACTCCCTGCCATTGTCTTCCAGATGTTTGAACAGAATCTTCAAGTTGCTGCTCAGATAAGTG
  AAGATTTGAAAACAAAGGTACTAGTTTTATGTCTTCAGCAGATGAATTCTTTCCTAAGCAGATATAAAGA
  TGAAGCGCAGCTGTATAAAGAAGAGCACCTGAGGAATCGGCAGCACCCTCACTGCTACGTTCAGTACATG
  ATCGCCATCATCAACAACTGCCAGACCTTCAAGGAATCCATAGTCAGTTTAAAAAGAAAGTATTTAAAGA
  ATGAAGTGGAAGAGGGTGTGTCTCCGAGCCAGCCCAGCATGGACGGGATTTTAGACGCCATCGCGAAGGA
  GGGCTGCAGCGGTTTGCTGGAGGAGGTCTTCCTGGACCTGGAGCAACATCTGAATGAATTGATGACGAAG
  AAGTGGCTATTAGGGTCAAACGCTGTAGACATTATCTGTGTCACCGTGGAAGACTATTTCAACGATTTTG
  CCAAAATTAAAAAGCCGTATAAGAAGAGGATGACGGCCGAGGCGCACCGGCGCGTGGTGGTGGAGTACCT
  GCGGGCGGTCATGCAGAAGCGCATTTCCTTCCGGAGCCCGGAGGAGCGCAAGGAGGGTGCCGAGAAGATG
  GTTAGGGAGGCAGAGCAGCTGCGCTTCCTGTTCCGGAAGCTGGCGTCCGGTTTCGGGGAAGACGTGGACG
  GATACTGCGACACCATCGTGGCTGTGGCCGAAGTGATCAAGCTGACAGACCCTTCTCTGCTCTACCTGGA
  GGTCTCCACTCTGGTCAGCAAGTATCCAGACATCAGGGATGACCACATCGGTGCGCTGCTGGCTGTGCGT
  GGGGACGCCAGCCGTGACATGAAGCAGACCATCATGGAGACCCTGGAGCAGGGCCCAGCACAGGCCAGCC
  CCAGCTACGTGCCCCTCTTCAAGGACATTGTGGTGCCCAGCCTGAACGTGGCCAAGCTGCTCAAG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC209413 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MKETDREAVATAVQRVAGMLQRPDQLDKVEQYRRREARKKASVEARLKAAIQSQLDGVRTGLSQLHNALN
  DVKDIQQSLADVSKDWRQSINTIESLKDVKDAVVQHSQLAAAVENLKNIFSVPEIVRETQDLIEQGALLQ
  AHRKLMDLECSRDGLMYEQYRMDSGNTRDMTLIHGYFGSTQGLSDELAKQLWMVLQRSLVTVRRDPTLLV
  SVVRIIEREEKIDRRILDRKKQTGFVPPGRPKNWKEKMFTILERTVTTRIEGTQADTRESDKMWLVRHLE
  IIRKYVLDDLIVAKNLMVQCFPPHYEIFKNLLNMYHQALSTRMQDLASEDLEANEIVSLLTWVLNTYTST
  EMMRNVELAPEVDVGTLEPLLSPHVVSELLDTYMSTLTSNIIAWLRKALETDKKDWVKETEPEADQDGYY
  QTTLPAIVFQMFEQNLQVAAQISEDLKTKVLVLCLQQMNSFLSRYKDEAQLYKEEHLRNRQHPHCYVQYM
  IAIINNCQTFKESIVSLKRKYLKNEVEEGVSPSQPSMDGILDAIAKEGCSGLLEEVFLDLEQHLNELMTK
  KWLLGSNAVDIICVTVEDYFNDFAKIKKPYKKRMTAEAHRRVVVEYLRAVMQKRISFRSPEERKEGAEKM
  VREAEQLRFLFRKLASGFGEDVDGYCDTIVAVAEVIKLTDPSLLYLEVSTLVSKYPDIRDDHIGALLAVR
  GDASRDMKQTIMETLEQGPAQASPSYVPLFKDIVVPSLNVAKLLK
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007277
• ORF Size: 2235 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007277.5
• RefSeq Size: 2774 bp
• RefSeq ORF: 2238 bp
• Locus ID: 11336
• UniProt ID: O60645
• Cytogenetics: 5p15.33
• Protein Pathways: Tight junction
• MW: 85.6 kDa
• Gene Summary: The protein encoded by this gene is a component of the exocyst complex, a multiple protein complex essential for targeting exocytic vesicles to specific docking sites on the plasma membrane. Though best characterized in yeast, the component proteins and functions of exocyst complex have been demonstrated to be highly conserved in higher eukaryotes. At least eight components of the exocyst complex, including this protein, are found to interact with the actin cytoskeletal remodeling and vesicle transport machinery. The complex is also essential for the biogenesis of epithelial cell surface polarity. [provided by RefSeq, Jul 2008]