RanBP16 (XPO7) (NM_015024) Human Tagged ORF Clone

CAT#: RC205979

XPO7 (Myc-DDK-tagged)-Human exportin 7 (XPO7)

Product Images

Circular map for RC205979

• 

  Western blot validation of overexpression lysate (Cat# LY402402) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC205979 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: RanBP16
• Synonyms: EXP7; RANBP16
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC205979 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGATCATGTGCAGAGCCTGGCCCAACTAGAGAATCTGTGCAAACAGCTGTATGAAACCACAGACA
  CAACCACTCGACTCCAGGCAGAGAAAGCCTTGGTTGAATTTACCAACAGCCCTGATTGCCTGAGCAAGTG
  CCAGCTACTCCTCGAAAGAGGAAGTTCCTCTTACTCCCAGTTACTGGCAGCTACATGCCTTACCAAGCTT
  GTATCACGCACAAACAACCCCCTACCATTGGAACAGCGAATAGATATTCGGAACTATGTGCTCAACTACC
  TTGCCACTCGGCCGAAGTTGGCTACTTTCGTGACACAAGCACTTATTCAGTTATATGCCAGAATCACAAA
  ACTGGGCTGGTTTGACTGTCAGAAGGATGACTATGTCTTCAGAAATGCAATCACAGACGTCACAAGGTTT
  TTACAGGATAGTGTTGAATACTGCATCATTGGTGTCACAATTTTATCTCAGCTAACCAATGAAATTAATC
  AAGCAGACACCACCCATCCTTTAACCAAGCACAGAAAAATAGCCTCTTCTTTTCGCGATTCATCATTATT
  TGATATCTTCACACTTTCCTGCAATTTACTAAAACAGGCTTCAGGAAAGAATCTAAACTTGAATGATGAA
  AGTCAGCATGGCTTGCTCATGCAACTGCTCAAGCTCACTCATAACTGCCTCAACTTTGACTTCATCGGCA
  CTTCCACTGATGAGTCCTCAGACGACCTGTGTACAGTGCAGATTCCCACCAGCTGGAGATCAGCCTTCTT
  AGATTCTTCAACCTTGCAGCTGTTTTTTGACCTGTATCATTCCATCCCTCCTTCATTTTCACCTCTGGTA
  TTATCCTGCTTGGTACAGATCGCTTCAGTCAGAAGATCCCTGTTTAACAATGCAGAGAGGGCCAAGTTTC
  TCTCTCATCTTGTTGATGGTGTTAAACGAATACTGGAAAACCCACAGAGTTTATCAGACCCAAACAATTA
  CCATGAGTTTTGCAGACTACTGGCCCGATTGAAGAGTAACTATCAACTGGGAGAATTGGTAAAGGTGGAA
  AACTACCCTGAGGTCATCCGATTGATAGCCAACTTCACAGTGACCAGCCTACAGCACTGGGAATTTGCTC
  CAAATAGTGTGCACTATCTTCTGAGCCTGTGGCAGCGGCTGGCAGCCTCTGTGCCGTATGTCAAAGCCAC
  AGACCCCCACATGCTGGAAACTTACACTCCTGAGGTCACCAAAGCCTACATCACATCCCGGTTGGAATCT
  GTGCACATCATACTGAGAGATGGCCTGGAAGATCCCCTGGAGGATACGGGGCTGGTCCAGCAGCAGTTGG
  ACCAGCTGTCCACCATTGGGCGTTGTGAATATGAGAAGACGTGTGCACTCCTCGTGCAGTTGTTTGACCA
  GTCGGCCCAGTCGTACCAGGAGCTGCTACAGAGCGCCAGCGCAAGCCCAATGGACATTGCAGTGCAGGAG
  GGAAGGCTGACATGGCTGGTTTACATTATTGGAGCAGTGATCGGTGGCCGGGTTTCTTTTGCCAGCACTG
  ATGAGCAAGACGCCATGGATGGTGAGCTTGTCTGTCGGGTGCTCCAGCTGATGAACCTAACAGATTCTCG
  TTTGGCCCAGGCGGGTAATGAGAAGCTAGAGTTGGCCATGCTGAGCTTTTTTGAACAGTTTCGTAAGATC
  TACATTGGGGACCAAGTGCAGAAATCCTCTAAGCTGTACCGCCGACTCTCAGAAGTTCTGGGCTTGAATG
  ATGAGACCATGGTCCTAAGCGTCTTCATAGGAAAAATCATCACCAACTTGAAGTACTGGGGCCGTTGTGA
  ACCAATCACCTCCAAGACACTACAGCTTCTCAATGACCTGTCCATTGGGTACAGTAGCGTAAGGAAGCTA
  GTGAAGCTTAGTGCGGTACAGTTCATGCTGAACAATCACACGAGCGAGCACTTTTCATTTTTGGGTATTA
  ACAATCAGTCCAACCTGACAGACATGCGGTGTCGGACTACCTTCTACACAGCACTTGGGCGTCTCCTCAT
  GGTGGATTTAGGAGAGGATGAAGATCAGTATGAGCAGTTCATGCTGCCACTCACAGCAGCATTTGAGGCT
  GTGGCCCAGATGTTTAGCACCAATAGTTTCAACGAGCAGGAGGCAAAGCGAACTCTAGTTGGCCTAGTAA
  GAGACCTGAGAGGGATCGCTTTCGCTTTCAATGCCAAGACCAGCTTCATGATGCTCTTTGAATGGATATA
  TCCATCCTATATGCCAATTCTCCAACGGGCAATTGAGCTCTGGTACCATGATCCAGCCTGTACTACACCT
  GTACTCAAGTTGATGGCTGAATTGGTTCATAATAGGTCCCAGCGACTCCAGTTTGATGTCTCTTCCCCCA
  ATGGCATCTTACTCTTCCGAGAAACCAGCAAGATGATAACAATGTATGGCAATCGCATCCTGACACTAGG
  AGAGGTCCCAAAGGATCAGGTCTATGCTCTGAAGCTCAAGGGCATCTCCATCTACTTCTCCATGCTGAAG
  GCTGCTCTCAGTGGGAGTTACGTCAATTTCGGAGTCTTTCGTCTCTATGGAGACGATGCCCTGGACAATG
  CTCTGCAGACCTTCATCAAGCTGCTCCTCTCTATTCCTCACAGTGATCTCTTGGATTACCCCAAGCTCAG
  CCAGTCTTATTATTCACTACTGGAAGTCCTGACCCAGGACCATATGAACTTTATTGCAAGCCTGGAACCT
  CACGTCATCATGTATATTCTCTCTTCCATTTCTGAAGGACTTACTGCACTTGACACCATGGTATGCACAG
  GCTGCTGCTCCTGCCTGGACCACATTGTGACATACCTCTTCAAGCAGCTGTCACGTAGCACCAAGAAGAG
  GACCACACCCCTGAACCAGGAGAGCGACCGCTTTCTGCACATCATGCAGCAGCATCCAGAGATGATCCAG
  CAGATGCTGTCCACGGTGCTGAACATCATCATCTTTGAAGACTGTAGGAACCAGTGGTCTATGTCCCGAC
  CACTACTTGGCTTGATATTGCTTAATGAAAAGTATTTTTCTGACCTAAGAAACAGTATTGTGAACAGCCA
  GCCACCGGAGAAGCAGCAGGCCATGCACCTGTGTTTTGAGAACCTGATGGAAGGCATCGAGCGAAATCTT
  CTTACGAAAAACAGAGACAGGTTCACCCAGAACCTGTCAGCATTCCGTCGAGAAGTCAACGACTCAATGA
  AGAATTCCACTTATGGCGTGAATAGCAATGACATGATGAGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC205979 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MADHVQSLAQLENLCKQLYETTDTTTRLQAEKALVEFTNSPDCLSKCQLLLERGSSSYSQLLAATCLTKL
  VSRTNNPLPLEQRIDIRNYVLNYLATRPKLATFVTQALIQLYARITKLGWFDCQKDDYVFRNAITDVTRF
  LQDSVEYCIIGVTILSQLTNEINQADTTHPLTKHRKIASSFRDSSLFDIFTLSCNLLKQASGKNLNLNDE
  SQHGLLMQLLKLTHNCLNFDFIGTSTDESSDDLCTVQIPTSWRSAFLDSSTLQLFFDLYHSIPPSFSPLV
  LSCLVQIASVRRSLFNNAERAKFLSHLVDGVKRILENPQSLSDPNNYHEFCRLLARLKSNYQLGELVKVE
  NYPEVIRLIANFTVTSLQHWEFAPNSVHYLLSLWQRLAASVPYVKATDPHMLETYTPEVTKAYITSRLES
  VHIILRDGLEDPLEDTGLVQQQLDQLSTIGRCEYEKTCALLVQLFDQSAQSYQELLQSASASPMDIAVQE
  GRLTWLVYIIGAVIGGRVSFASTDEQDAMDGELVCRVLQLMNLTDSRLAQAGNEKLELAMLSFFEQFRKI
  YIGDQVQKSSKLYRRLSEVLGLNDETMVLSVFIGKIITNLKYWGRCEPITSKTLQLLNDLSIGYSSVRKL
  VKLSAVQFMLNNHTSEHFSFLGINNQSNLTDMRCRTTFYTALGRLLMVDLGEDEDQYEQFMLPLTAAFEA
  VAQMFSTNSFNEQEAKRTLVGLVRDLRGIAFAFNAKTSFMMLFEWIYPSYMPILQRAIELWYHDPACTTP
  VLKLMAELVHNRSQRLQFDVSSPNGILLFRETSKMITMYGNRILTLGEVPKDQVYALKLKGISIYFSMLK
  AALSGSYVNFGVFRLYGDDALDNALQTFIKLLLSIPHSDLLDYPKLSQSYYSLLEVLTQDHMNFIASLEP
  HVIMYILSSISEGLTALDTMVCTGCCSCLDHIVTYLFKQLSRSTKKRTTPLNQESDRFLHIMQQHPEMIQ
  QMLSTVLNIIIFEDCRNQWSMSRPLLGLILLNEKYFSDLRNSIVNSQPPEKQQAMHLCFENLMEGIERNL
  LTKNRDRFTQNLSAFRREVNDSMKNSTYGVNSNDMMS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_015024
• ORF Size: 3261 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015024.3
• RefSeq Size: 4880 bp
• RefSeq ORF: 3264 bp
• Locus ID: 23039
• UniProt ID: Q9UIA9
• Cytogenetics: 8p21.3
• Domains: IBN_NT
• Protein Families: Druggable Genome
• MW: 124 kDa
• Gene Summary: The transport of protein and large RNAs through the nuclear pore complexes (NPC) is an energy-dependent and regulated process. The import of proteins with a nuclear localization signal (NLS) is accomplished by recognition of one or more clusters of basic amino acids by the importin-alpha/beta complex; see MIM 600685 and MIM 602738. The small GTPase RAN (MIM 601179) plays a key role in NLS-dependent protein import. RAN-binding protein-16 is a member of the importin-beta superfamily of nuclear transport receptors.[supplied by OMIM, Jul 2002]