AHCYL1 (NM_006621) Human Tagged ORF Clone

CAT#: RC201940

AHCYL1 (Myc-DDK-tagged)-Homo sapiens adenosylhomocysteinase-like 1 (AHCYL1), transcript variant 1

Product Images

Circular map for RC201940

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  Western blot validation of overexpression lysate (Cat# LY416510) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC201940 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified AHCYL1 protein (Cat# TP301940). The protein was produced from HEK293T cells transfected with AHCYL1 cDNA clone (Cat# RC201940) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: AHCYL1
• Synonyms: DCAL; IRBIT; PPP1R78; PRO0233; XPVKONA
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC201940 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  CTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCCGGCGC
  GCCC
  
  ATGTCGATGCCTGACGCGATGCCGCTGCCCGGGGTCGGGGAGGAGCTGAAGCAGGCCAAGGAGATCGAGG
  ACGCCGAGAAGTACTCCTTCATGGCCACCGTCACCAAGGCGCCCAAGAAGCAAATCCAGTTTGCTGATGA
  CATGCAGGAGTTCACCAAATTCCCCACCAAAACTGGCCGAAGATCTTTGTCTCGCTCGATCTCACAGTCC
  TCCACTGACAGCTACAGTTCAGCTGCATCCTACACAGATAGCTCTGATGATGAGGTTTCTCCCCGAGAGA
  AGCAGCAAACCAACTCCAAGGGCAGCAGCAATTTCTGTGTGAAGAACATCAAGCAGGCAGAATTTGGACG
  CCGGGAGATTGAGATTGCAGAGCAAGACATGTCTGCTCTGATTTCACTCAGGAAACGTGCTCAGGGGGAG
  AAGCCCTTGGCTGGTGCTAAAATAGTGGGCTGTACACACATCACAGCCCAGACAGCGGTGTTGATTGAGA
  CACTCTGTGCCCTGGGGGCTCAGTGCCGCTGGTCTGCTTGTAACATCTACTCAACTCAGAATGAAGTAGC
  TGCAGCACTGGCTGAGGCTGGAGTTGCAGTGTTCGCTTGGAAGGGCGAGTCAGAAGATGACTTCTGGTGG
  TGTATTGACCGCTGTGTGAACATGGATGGGTGGCAGGCCAACATGATCCTGGATGATGGGGGAGACTTAA
  CCCACTGGGTTTATAAGAAGTATCCAAACGTGTTTAAGAAGATCCGAGGCATTGTGGAAGAGAGCGTGAC
  TGGTGTTCACAGGCTGTATCAGCTCTCCAAAGCTGGGAAGCTCTGTGTTCCGGCCATGAACGTCAATGAT
  TCTGTTACCAAACAGAAGTTTGATAACTTGTACTGCTGCCGAGAATCCATTTTGGATGGCCTGAAGAGGA
  CCACAGATGTGATGTTTGGTGGGAAACAAGTGGTGGTGTGTGGCTATGGTGAGGTAGGCAAGGGCTGCTG
  TGCTGCTCTCAAAGCTCTTGGAGCAATTGTCTACATTACCGAAATCGACCCCATCTGTGCTCTGCAGGCC
  TGCATGGATGGGTTCAGGGTGGTAAAGCTAAATGAAGTCATCCGGCAAGTCGATGTCGTAATAACTTGCA
  CAGGAAATAAGAATGTAGTGACACGGGAGCACTTGGATCGCATGAAAAACAGTTGTATCGTATGCAATAT
  GGGCCACTCCAACACAGAAATCGATGTGACCAGCCTCCGCACTCCGGAGCTGACGTGGGAGCGAGTACGT
  TCTCAGGTGGACCATGTCATCTGGCCAGATGGCAAACGAGTTGTCCTCCTGGCAGAGGGTCGTCTACTCA
  ATTTGAGCTGCTCCACAGTTCCCACCTTTGTTCTGTCCATCACAGCCACAACACAGGCTTTGGCACTGAT
  AGAACTCTATAATGCACCCGAGGGGCGATACAAGCAGGATGTGTACTTGCTTCCTAAGAAAATGGATGAA
  TACGTTGCCAGCTTGCATCTGCCATCATTTGATGCCCACCTTACAGAGCTGACAGATGACCAAGCAAAAT
  ATCTGGGACTCAACAAAAATGGGCCATTCAAACCTAATTATTACAGATAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC201940 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSMPDAMPLPGVGEELKQAKEIEDAEKYSFMATVTKAPKKQIQFADDMQEFTKFPTKTGRRSLSRSISQS
  STDSYSSAASYTDSSDDEVSPREKQQTNSKGSSNFCVKNIKQAEFGRREIEIAEQDMSALISLRKRAQGE
  KPLAGAKIVGCTHITAQTAVLIETLCALGAQCRWSACNIYSTQNEVAAALAEAGVAVFAWKGESEDDFWW
  CIDRCVNMDGWQANMILDDGGDLTHWVYKKYPNVFKKIRGIVEESVTGVHRLYQLSKAGKLCVPAMNVND
  SVTKQKFDNLYCCRESILDGLKRTTDVMFGGKQVVVCGYGEVGKGCCAALKALGAIVYITEIDPICALQA
  CMDGFRVVKLNEVIRQVDVVITCTGNKNVVTREHLDRMKNSCIVCNMGHSNTEIDVTSLRTPELTWERVR
  SQVDHVIWPDGKRVVLLAEGRLLNLSCSTVPTFVLSITATTQALALIELYNAPEGRYKQDVYLLPKKMDE
  YVASLHLPSFDAHLTELTDDQAKYLGLNKNGPFKPNYYRY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: AscI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006621
• ORF Size: 1590 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006621.7
• RefSeq Size: 3949 bp
• RefSeq ORF: 1593 bp
• Locus ID: 10768
• UniProt ID: O43865
• Cytogenetics: 1p13.3
• Domains: AdoHcyase
• Protein Families: Druggable Genome
• Protein Pathways: Cysteine and methionine metabolism, Metabolic pathways, Selenoamino acid metabolism
• MW: 59 kDa
• Gene Summary: The protein encoded by this gene interacts with inositol 1,4,5-trisphosphate receptor, type 1 and may be involved in the conversion of S-adenosyl-L-homocysteine to L-homocysteine and adenosine. Several transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jun 2011]