GAS41 (YEATS4) (NM_006530) Human Tagged ORF Clone

CAT#: RC201526

YEATS4 (Myc-DDK-tagged)-Human YEATS domain containing 4 (YEATS4)

Product Images

Circular map for RC201526

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  Western blot validation of overexpression lysate (Cat# LY401956) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC201526 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified YEATS4 protein (Cat# TP301526). The protein was produced from HEK293T cells transfected with YEATS4 cDNA clone (Cat# RC201526) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: GAS41
• Synonyms: 4930573H17Rik; B230215M10Rik; GAS41; NUBI-1; YAF9
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC201526 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTCAAGAGAATGGCCGAATTTGGGCCTGACTCCGGCGGGAGAGTAAAGGGTGTTACTATCGTTAAAC
  CAATAGTTTACGGTAATGTTGCTCGGTATTTTGGAAAGAAAAGAGAAGAAGATGGGCACACTCATCAGTG
  GACAGTATATGTGAAACCATATAGAAATGAGGATATGTCAGCATATGTGAAGAAAATCCAGTTTAAATTA
  CATGAAAGCTATGGCAATCCTTTAAGAGTTGTTACTAAACCTCCATATGAAATTACTGAAACAGGATGGG
  GTGAATTCGAAATAATCATCAAAATATTTTTCATTGACCCTAATGAAAGACCTGTAACCCTGTATCATTT
  GCTAAAGCTGTTTCAATCAGACACCAATGCAATGCTGGGGAAAAAGACAGTGGTTTCAGAGTTCTATGAT
  GAAATGATATTTCAAGACCCAACAGCAATGATGCAACAATTATTGACAACATCTCGTCAGCTAACATTAG
  GAGCCTATAAGCATGAAACAGAATTTGCAGAGCTTGAAGTGAAAACCAGAGAAAAATTAGAAGCTGCTAA
  GAAAAAAACAAGCTTTGAGATTGCAGAGCTTAAGGAGAGATTAAAAGCAAGTCGTGAAACTATAAATTGT
  TTAAAAAATGAAATCAGAAAACTTGAAGAAGATGACCAAGCAAAAGACATA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC201526 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MFKRMAEFGPDSGGRVKGVTIVKPIVYGNVARYFGKKREEDGHTHQWTVYVKPYRNEDMSAYVKKIQFKL
  HESYGNPLRVVTKPPYEITETGWGEFEIIIKIFFIDPNERPVTLYHLLKLFQSDTNAMLGKKTVVSEFYD
  EMIFQDPTAMMQQLLTTSRQLTLGAYKHETEFAELEVKTREKLEAAKKKTSFEIAELKERLKASRETINC
  LKNEIRKLEEDDQAKDI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006530
• ORF Size: 681 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006530.4
• RefSeq Size: 1509 bp
• RefSeq ORF: 684 bp
• Locus ID: 8089
• UniProt ID: O95619
• Cytogenetics: 12q15
• Domains: YEATS
• Protein Families: Druggable Genome, Transcription Factors
• MW: 26.5 kDa
• Gene Summary: The protein encoded by this gene is found in the nucleoli. It has high sequence homology to human MLLT1, and yeast and human MLLT3 proteins. Both MLLT1 and MLLT3 proteins belong to a class of transcription factors, indicating that the encoded protein might also represent a transcription factor. This protein is thought to be required for RNA transcription. This gene has been shown to be amplified in tumors. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2014]