KAP1 (TRIM28) (NM_005762) Human Tagged ORF Clone

CAT#: RC201205

TRIM28 (Myc-DDK-tagged)-Human tripartite motif containing 28 (TRIM28)

Product Images

Circular map for RC201205

• 

  Western blot validation of overexpression lysate (Cat# LY417086) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC201205 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified TRIM28 protein (Cat# TP301205). The protein was produced from HEK293T cells transfected with TRIM28 cDNA clone (Cat# RC201205) using MegaTran 2.0 (Cat# TT210002).

• 

  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY TRIM28 (Cat# RC201205, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TRIM28 (Cat# TA813318)(1:500).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: KAP1
• Synonyms: KAP1; PPP1R157; RNF96; TF1B; TIF1B
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC201205 representing NM_005762
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCCTCCGCGGCGGCAGCCTCGGCAGCAGCGGCCTCGGCCGCCTCTGGCAGCCCGGGCCCGGGCG
  AGGGCTCCGCTGGCGGCGAAAAGCGCTCCACCGCCCCTTCGGCCGCAGCCTCGGCCTCTGCCTCAGCCGC
  GGCGTCGTCGCCCGCGGGGGGCGGCGCCGAGGCGCTGGAGCTGCTGGAGCACTGCGGCGTGTGCAGAGAG
  CGCCTGCGACCCGAGAGGGAGCCCCGCCTGCTGCCCTGTTTGCACTCGGCCTGTAGTGCCTGCTTAGGGC
  CCGCGGCCCCCGCCGCCGCCAACAGCTCGGGGGACGGCGGGGCGGCGGGCGACGGCACCGTGGTGGACTG
  TCCCGTGTGCAAGCAACAGTGCTTCTCCAAAGACATCGTGGAGAATTATTTCATGCGTGATAGTGGCAGC
  AAGGCTGCCACCGACGCCCAGGATGCGAACCAGTGCTGCACTAGCTGTGAGGATAATGCCCCAGCCACCA
  GCTACTGTGTGGAGTGCTCGGAGCCTCTGTGTGAGACCTGTGTAGAGGCGCACCAGCGGGTGAAGTACAC
  CAAGGACCATACTGTGCGCTCTACTGGGCCAGCCAAGTCTCGGGATGGTGAACGTACTGTCTATTGCAAC
  GTACACAAGCATGAACCCCTTGTGCTGTTTTGTGAGAGCTGTGATACTCTCACCTGCCGAGACTGCCAGC
  TCAATGCCCACAAGGACCACCAGTACCAGTTCTTAGAGGATGCAGTGAGGAACCAGCGCAAGCTCCTGGC
  CTCACTGGTGAAGCGCCTTGGGGACAAACATGCAACATTGCAGAAGAGCACCAAGGAGGTTCGCAGCTCA
  ATCCGCCAGGTGTCTGACGTACAGAAGCGTGTGCAAGTGGATGTCAAGATGGCCATCCTGCAGATCATGA
  AGGAGCTGAATAAGCGGGGCCGTGTGCTGGTCAATGATGCCCAGAAGGTGACTGAGGGGCAGCAGGAGCG
  CCTGGAGCGGCAGCACTGGACCATGACCAAGATCCAGAAGCACCAGGAGCACATTCTGCGCTTTGCCTCT
  TGGGCTCTGGAGAGTGACAACAACACAGCCCTTTTGCTTTCTAAGAAGTTGATCTACTTCCAGCTGCACC
  GGGCCCTCAAGATGATTGTGGATCCCGTGGAGCCACATGGCGAGATGAAGTTTCAGTGGGACCTCAATGC
  CTGGACCAAGAGTGCCGAGGCCTTTGGCAAGATTGTGGCAGAGCGTCCTGGCACTAACTCAACAGGCCCT
  GCACCCATGGCCCCTCCAAGAGCCCCAGGGCCCCTGAGCAAGCAGGGCTCTGGCAGCAGCCAGCCCATGG
  AGGTGCAGGAAGGCTATGGCTTTGGGTCAGGAGATGATCCCTACTCAAGTGCAGAGCCCCATGTGTCAGG
  TGTGAAACGGTCCCGCTCAGGTGAGGGCGAGGTGAGCGGCCTTATGCGCAAGGTGCCACGAGTGAGCCTT
  GAACGCCTGGACCTGGACCTCACAGCTGACAGCCAGCCACCCGTCTTCAAGGTCTTCCCAGGCAGTACCA
  CTGAGGACTACAACCTTATTGTTATTGAACGTGGCGCTGCCGCTGCAGCTACCGGCCAGCCAGGGACTGC
  GCCTGCAGGAACCCCTGGTGCCCCACCCCTGGCTGGCATGGCCATTGTCAAGGAGGAGGAGACGGAGGCT
  GCCATTGGAGCCCCTCCTACTGCCACTGAGGGCCCTGAGACCAAACCTGTGCTTATGGCTCTTGCGGAGG
  GTCCTGGTGCTGAGGGTCCCCGCCTGGCCTCACCTAGTGGCAGCACCAGCTCAGGGCTGGAGGTGGTGGC
  TCCTGAGGGTACCTCAGCCCCAGGTGGTGGCCCGGGAACCCTGGATGACAGTGCCACCATTTGCCGTGTC
  TGCCAGAAGCCAGGCGATCTGGTTATGTGCAACCAGTGTGAGTTTTGTTTCCACCTGGACTGTCACCTGC
  CGGCCCTGCAGGATGTACCAGGGGAGGAGTGGAGCTGCTCACTCTGCCATGTGCTCCCTGACCTGAAGGA
  GGAGGATGGCAGCCTCAGCCTGGATGGTGCAGACAGCACTGGCGTGGTGGCCAAGCTCTCACCAGCCAAC
  CAGCGGAAATGTGAGCGTGTACTGCTGGCCCTATTCTGTCACGAACCCTGCCGCCCCCTGCATCAGCTGG
  CTACCGACTCCACCTTCTCCCTGGACCAGCCCGGTGGCACCCTGGATCTGACCCTGATCCGTGCCCGCCT
  CCAGGAGAAGTTGTCACCTCCCTACAGCTCCCCACAGGAGTTTGCCCAGGATGTGGGCCGCATGTTCAAG
  CAATTCAACAAGTTAACTGAGGACAAGGCAGACGTGCAGTCCATCATCGGCCTGCAGCGCTTCTTCGAGA
  CGCGCATGAACGAGGCCTTCGGTGACACCAAGTTCTCTGCTGTGCTGGTGGAGCCCCCGCCGATGAGCCT
  GCCTGGTGCTGGCCTGAGTTCCCAGGAGCTGTCTGGTGGCCCTGGTGATGGCCCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC201205 representing NM_005762
  Red=Cloning site Green=Tags(s)
  
  MAASAAAASAAAASAASGSPGPGEGSAGGEKRSTAPSAAASASASAAASSPAGGGAEALELLEHCGVCRE
  RLRPEREPRLLPCLHSACSACLGPAAPAAANSSGDGGAAGDGTVVDCPVCKQQCFSKDIVENYFMRDSGS
  KAATDAQDANQCCTSCEDNAPATSYCVECSEPLCETCVEAHQRVKYTKDHTVRSTGPAKSRDGERTVYCN
  VHKHEPLVLFCESCDTLTCRDCQLNAHKDHQYQFLEDAVRNQRKLLASLVKRLGDKHATLQKSTKEVRSS
  IRQVSDVQKRVQVDVKMAILQIMKELNKRGRVLVNDAQKVTEGQQERLERQHWTMTKIQKHQEHILRFAS
  WALESDNNTALLLSKKLIYFQLHRALKMIVDPVEPHGEMKFQWDLNAWTKSAEAFGKIVAERPGTNSTGP
  APMAPPRAPGPLSKQGSGSSQPMEVQEGYGFGSGDDPYSSAEPHVSGVKRSRSGEGEVSGLMRKVPRVSL
  ERLDLDLTADSQPPVFKVFPGSTTEDYNLIVIERGAAAAATGQPGTAPAGTPGAPPLAGMAIVKEEETEA
  AIGAPPTATEGPETKPVLMALAEGPGAEGPRLASPSGSTSSGLEVVAPEGTSAPGGGPGTLDDSATICRV
  CQKPGDLVMCNQCEFCFHLDCHLPALQDVPGEEWSCSLCHVLPDLKEEDGSLSLDGADSTGVVAKLSPAN
  QRKCERVLLALFCHEPCRPLHQLATDSTFSLDQPGGTLDLTLIRARLQEKLSPPYSSPQEFAQDVGRMFK
  QFNKLTEDKADVQSIIGLQRFFETRMNEAFGDTKFSAVLVEPPPMSLPGAGLSSQELSGGPGDGP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005762
• ORF Size: 2505 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005762.3
• RefSeq Size: 2989 bp
• RefSeq ORF: 2508 bp
• Locus ID: 10155
• UniProt ID: Q13263
• Cytogenetics: 19q13.43
• Domains: zf-B_box, BROMO, RING, PHD, BBC
• Protein Families: Protein Kinase, Stem cell - Pluripotency, Transcription Factors
• MW: 88.4 kDa
• Gene Summary: The protein encoded by this gene mediates transcriptional control by interaction with the Kruppel-associated box repression domain found in many transcription factors. The protein localizes to the nucleus and is thought to associate with specific chromatin regions. The protein is a member of the tripartite motif family. This tripartite motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. [provided by RefSeq, Jul 2008]