RPC62 (POLR3C) (NM_006468) Human Tagged ORF Clone

CAT#: RC200279

POLR3C (Myc-DDK-tagged)-Human polymerase (RNA) III (DNA directed) polypeptide C (62kD) (POLR3C)

Product Images

Circular map for RC200279

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY POLR3C (Cat# RC200279, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-POLR3C(Cat# TA505106). Positive lysates LY401943 (100ug) and LC401943 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY401943) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC200279 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified POLR3C protein (Cat# TP300279). The protein was produced from HEK293T cells transfected with POLR3C cDNA clone (Cat# RC200279) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: RPC62
• Synonyms: C82; RPC3; RPC62
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC200279 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACTCAAGCAGAAATTAAGCTCTGTTCTTTGTTGCTGCAAGAGCATTTTGGAGAGATTGTAGAAAAAA
  TTGGAGTCCATCTGATAAGAACCGGCAGCCAGCCACTAAGAGTAATTGCCCATGACACAGGAACATCACT
  GGATCAGGTGAAGAAAGCCCTGTGTGTCCTCGTCCAACATAACCTGGTGAGTTATCAAGTGCACAAACGT
  GGTGTGGTGGAGTATGAAGCCCAGTGCAGCCGGGTATTGCGAATGCTTAGATATCCCCGGTACATCTATA
  CTACCAAAACTCTGTACAGTGACACTGGAGAGCTGATTGTTGAGGAGCTTCTGTTGAACGGCAAACTGAC
  AATGTCAGCTGTTGTGAAGAAAGTGGCAGACCGGCTCACAGAGACCATGGAGGATGGCAAGACCATGGAC
  TATGCTGAAGTATCAAACACATTTGTGCGACTGGCAGACACACACTTTGTACAACGCTGCCCTTCGGTAC
  CTACCACTGAGAATTCAGACCCTGGGCCACCACCACCTGCCCCCACACTTGTCATTAATGAAAAGGACAT
  GTACCTGGTTCCTAAACTCAGCTTGATAGGGAAAGGTAAAAGGAGGAGATCATCTGATGAAGATGCTGCT
  GGGGAGCCCAAGGCCAAGAGACCAAAATATACTACAGATAACAAGGAGCCCATTCCAGATGATGGGATTT
  ATTGGCAGGCCAACCTTGACAGATTCCACCAACACTTCCGTGACCAAGCCATTGTGAGCGCAGTTGCTAA
  CAGGATGGACCAGACAAGCAGCGAGATTGTGCGAACCATGCTCCGAATGAGTGAGATTACCACTTCCTCT
  AGTGCTCCCTTCACCCAGCCATTGTCTTCCAATGAGATCTTCAGATCCCTACCTGTTGGCTATAACATCT
  CTAAGCAAGTTCTTGATCAGTATCTCACTCTGCTGGCAGATGATCCACTAGAGTTTGTTGGAAAGTCTGG
  CGACAGTGGTGGAGGAATGTATGTCATCAACCTCCATAAGGCATTAGCATCCCTAGCCACAGCCACTCTG
  GAGTCCGTCGTACAGGAGAGATTTGGGTCTCGCTGTGCTAGAATATTCCGTCTAGTTTTGCAGAAGAAAC
  ACATAGAGCAGAAGCAAGTGGAAGACTTTGCAATGATTCCTGCAAAGGAGGCAAAGGATATGCTATATAA
  GATGCTCTCAGAAAATTTCATGTCACTCCAGGAAATTCCCAAAACACCAGACCATGCCCCATCCAGGACC
  TTCTATTTATATACTGTGAACATCCTGTCAGCTGCCCGAATGTTGTTGCACAGGTGCTACAAGAGCATAG
  CCAACTTGATAGAAAGGAGGCAATTTGAAACCAAAGAGAATAAGCGTCTACTAGAAAAATCTCAGAGGGT
  AGAAGCCATCATTGCATCTATGCAGGCTACTGGTGCAGAGGAAGCACAGTTACAAGAAATAGAGGAGATG
  ATCACAGCTCCTGAACGTCAGCAGCTAGAGACCCTGAAACGTAATGTCAACAAGTTGGATGCCAGTGAGA
  TCCAGGTGGACGAAACCATCTTCCTGCTGGAGTCTTACATTGAGTGCACCATGAAGAGACAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC200279 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MTQAEIKLCSLLLQEHFGEIVEKIGVHLIRTGSQPLRVIAHDTGTSLDQVKKALCVLVQHNLVSYQVHKR
  GVVEYEAQCSRVLRMLRYPRYIYTTKTLYSDTGELIVEELLLNGKLTMSAVVKKVADRLTETMEDGKTMD
  YAEVSNTFVRLADTHFVQRCPSVPTTENSDPGPPPPAPTLVINEKDMYLVPKLSLIGKGKRRRSSDEDAA
  GEPKAKRPKYTTDNKEPIPDDGIYWQANLDRFHQHFRDQAIVSAVANRMDQTSSEIVRTMLRMSEITTSS
  SAPFTQPLSSNEIFRSLPVGYNISKQVLDQYLTLLADDPLEFVGKSGDSGGGMYVINLHKALASLATATL
  ESVVQERFGSRCARIFRLVLQKKHIEQKQVEDFAMIPAKEAKDMLYKMLSENFMSLQEIPKTPDHAPSRT
  FYLYTVNILSAARMLLHRCYKSIANLIERRQFETKENKRLLEKSQRVEAIIASMQATGAEEAQLQEIEEM
  ITAPERQQLETLKRNVNKLDASEIQVDETIFLLESYIECTMKRQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006468
• ORF Size: 1602 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006468.8
• RefSeq Size: 1888 bp
• RefSeq ORF: 1605 bp
• Locus ID: 10623
• UniProt ID: Q9BUI4
• Cytogenetics: 1q21.1
• Protein Families: Transcription Factors
• Protein Pathways: Cytosolic DNA-sensing pathway, Metabolic pathways, Purine metabolism, Pyrimidine metabolism, RNA polymerase
• MW: 60.6 kDa
• Gene Summary: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. May direct with other members of the subcomplex RNA Pol III binding to the TFIIIB-DNA complex via the interactions between TFIIIB and POLR3F. May be involved either in the recruitment and stabilization of the subcomplex within RNA polymerase III, or in stimulating catalytic functions of other subunits during initiation. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway. Preferentially binds single-stranded DNA (ssDNA) in a sequence-independent manner (PubMed:21358628).[UniProtKB/Swiss-Prot Function]