Tubb1 (NM_001080971) Mouse Tagged ORF Clone

CAT#: MR224088

Tubb1 (Myc-DDK-tagged) - Mouse tubulin, beta 1 (Tubb1)

Product Images

Circular map for MR224088

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Tubb1
• Synonyms: 2810484G07Rik; M(beta)1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR224088 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGAGAGATCGTGCACATCCAGATTGGCCAGTGTGGCAACCAGATCGGAGCCAAGTTCTGGGAGGTGA
  TCGGGGAAGAACATGGGATTGACTGCGCTGGGAGCTACTGTGGGACGTCTGCTCTCCAGCTGGAGAGGAT
  CAGCGTTTACTACAACGAAGCCTACGGTAAGAAGTATGTGCCGCGAGCCGTCCTTGTGGACCTGGAACCC
  GGGACAATGGACAGCATCCGATCCAGCAGATTGGGAGTCCTCTTTCAGCCTGACAGTTTTGTTCATGGTA
  ACTCTGGTGCTGGTAACAACTGGGCGAAGGGCCACTACACAGAGGGAGCGGAGCTGATCGAAAATGTCAT
  GGATGTGGTAAGGAGGGAGAGCGAGAGCTGCGACTGCCTACAGGGTTTCCAGATCGTGCATTCTCTGGGT
  GGAGGCACGGGCTCAGGCATGGGCACCCTGCTCATGAACAAGATCAGGGAGGAGTACCCGGATCGGATCT
  TGAACTCCTTTAGCGTCATGCCATCACCCAAGGTATCAGACACGGTGGTAGAGCCCTACAATGCCGTGCT
  ATCCATCCACCAGCTGATAGAGAACACAGATGCCTGCTTCTGCATCGACAACGAAGCGCTCTATGACATT
  TGTTTCCGCACCCTGAGGCTGACGACACCCACCTACGGGGATCTCAACCACCTGGTGTCCTTGACCATGA
  GCGGAATCACGACTTCACTGCGCTTTCCCGGTCAGCTCAATGCAGACCTGCGCAAGCTGGCCGTGAATAT
  GGTGCCCTTTCCTCGTCTTCACTTCTTCATGCCTGGCTTCGCTCCACTCACAGCCCAGGGTAGCCAGCAG
  TACCGAGCCCTCTCAGTAGCGGAGCTCACCCAACAGATGTTCGATGCCCGCAACATCATGGCTGCCTGTG
  ACCCTCGCCGCGGCCGCTACCTGACTGTGGCTTGTATATTCCGGGGTAAGATGTCCACCAAGGAAGTGGA
  CCAACAGCTGCTGTCCATTCAGACAAGGAACAGCAACTGCTTTGTGGAATGGATTCCCAACAATGTCAAG
  GTAGCTGTGTGTGACATCCCGCCCCGTGGGCTGAACATGGCAGCTACCTTCCTGGGCAACAATACAGCCA
  TCCAAGAGCTCTTCACCAGGGTCTCTGAGCACTTCTCAGCTATGTTCAGAAGGAGAGCTTTTGTGCACTG
  GTACACCAGTGAAGGGATGGACATAAGTGAATTTGGGGAAGCTGAGAGTGATATCCATGACCTGGTGTCT
  GAGTACCAACAATTCCAAGATGTCAGAGCAGGGCTAGAGGACAGTGAAGAGGATGTGGAGGAGGCAGAGG
  TGGAGGCAGAAGACAAAGATCAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR224088 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MREIVHIQIGQCGNQIGAKFWEVIGEEHGIDCAGSYCGTSALQLERISVYYNEAYGKKYVPRAVLVDLEP
  GTMDSIRSSRLGVLFQPDSFVHGNSGAGNNWAKGHYTEGAELIENVMDVVRRESESCDCLQGFQIVHSLG
  GGTGSGMGTLLMNKIREEYPDRILNSFSVMPSPKVSDTVVEPYNAVLSIHQLIENTDACFCIDNEALYDI
  CFRTLRLTTPTYGDLNHLVSLTMSGITTSLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTAQGSQQ
  YRALSVAELTQQMFDARNIMAACDPRRGRYLTVACIFRGKMSTKEVDQQLLSIQTRNSNCFVEWIPNNVK
  VAVCDIPPRGLNMAATFLGNNTAIQELFTRVSEHFSAMFRRRAFVHWYTSEGMDISEFGEAESDIHDLVS
  EYQQFQDVRAGLEDSEEDVEEAEVEAEDKDH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001080971
• ORF Size: 1356 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001080971.2, NP_001074440.1
• RefSeq Size: 2004 bp
• RefSeq ORF: 1356 bp
• Locus ID: 545486
• UniProt ID: A2AQ07
• Cytogenetics: 2 H4
• MW: 50.4 kDa
• Gene Summary: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain (By similarity).[UniProtKB/Swiss-Prot Function]