Dyrk1a (NM_007890) Mouse Tagged ORF Clone

CAT#: MR221655

Dyrk1a (Myc-DDK-tagged) - Mouse dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 1a (Dyrk1a), transcript variant 1

Product Images

Circular map for MR221655

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Dyrk1a
• Synonyms: 2310043O08Rik; D16Ertd272e; D16Ertd493e; Dyrk; ENSMUSG00000074897; Gm10783; mmb; Mnbh; Mp86
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR221655 representing NM_007890
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCATACAGGAGGAGAGACTTCAGCATGCAAACCTTCATCTGTCCGGCTTGCACCGTCGTTCTCATTCC
  ATGCTGCTGGCCTTCAGATGGCTGCACAGATGCCCCACTCACACCAGTACAGTGACCGTCGCCAGCCGAG
  CATAAGTGACCAGCAGGTGTCTGCCTTACCATATTCTGACCAGATTCAGCAACCTCTAACTAACCAGGTG
  ATGCCTGACATTGTCATGTTACAGAGGCGGATGCCCCAAACCTTCCGTGATCCAGCAACTGCTCCTCTGA
  GAAAACTCTCTGTGGACTTGATCAAAACATACAAGCATATTAATGAGGTTTACTATGCAAAAAAGAAGCG
  AAGACACCAACAGGGCCAGGGGGACGATTCCAGTCATAAGAAGGAGCGGAAGGTTTACAATGATGGTTAC
  GATGATGATAACTATGATTATATTGTAAAAAACGGGGAAAAGTGGATGGATCGGTATGAAATCGACTCCT
  TAATAGGCAAAGGTTCATTTGGACAGGTTGTGAAAGCTTATGACAGAGTGGAGCAAGAATGGGTCGCCAT
  TAAAATCATCAAGAACAAGAAAGCGTTTCTGAATCAAGCCCAGATAGAAGTGCGGCTGCTTGAGCTCATG
  AACAAACACGACACTGAAATGAAGTACTACATAGTGCATTTGAAACGCCACTTTATGTTTCGAAACCATC
  TCTGTTTAGTGTTTGAAATGCTGTCCTATAATCTCTATGATTTGTTGAGAAACACCAACTTCCGAGGGGT
  CTCTTTGAACCTAACACGAAAGTTTGCGCAACAGATGTGCACAGCATTGCTTTTTCTTGCGACTCCAGAA
  CTTAGTATCATTCACTGTGACTTAAAGCCTGAGAACATCCTTCTGTGTAACCCCAAACGGAGTGCAATCA
  AGATTGTTGATTTTGGCAGCTCTTGTCAGTTGGGGCAGAGGATATACCAGTATATTCAGAGTCGCTTTTA
  TCGGTCTCCAGAGGTGCTACTGGGAATGCCTTATGACCTTGCTATCGACATGTGGTCCCTTGGATGTATC
  TTGGTTGAAATGCACACTGGAGAGCCTCTGTTCAGTGGTGCCAATGAGGTCGATCAGATGAATAAAATAG
  TGGAAGTCTTGGGCATCCCACCTGCTCATATTCTTGACCAAGCACCGAAAGCAAGAAAGTTCTTTGAGAA
  GTTGCCCGATGGCACTTGGAGCTTAAAGAAGACCAAAGATGGAAAACGGGAGTACAAACCACCAGGAACC
  CGTAAACTTCATAATATTCTTGGAGTAGAAACAGGAGGACCTGGCGGGCGGCGTGCTGGGGAATCGGGTC
  ATACTGTAGCTGACTACTTGAAGTTCAAAGACCTCATTTTAAGGATGCTTGATTATGACCCCAAAACTCG
  GATTCAACCTTATTATGCCCTGCAGCACAGTTTTTTCAAGAAAACAGCTGATGAAGGTACCAACACAAGT
  AACAGTGTGTCTACCAGCCCTGCGATGGAGCAGTCTCAGTCTTCAGGCACCACCTCCAGCACCTCCTCCA
  GCTCAGGTGGATCCTCGGGAACGAGTAACAGTGGGAGAGCCAGGTCGGATCCGACGCACCAGCATCGACA
  CAGCGGTGGACACTTCGCTGCTGCTGTCCAGGCCATGGACTGTGAGACACACAGTCCCCAGGTGCGCCAG
  CAGTTTCCGGCTCCTCTGGGATGGTCAGGCACTGAAGCTCCTACACAAGTCACTGTTGAAACTCATCCTG
  TTCAAGAGACAACCTTTCATGTAGCCCCCCAGCAGAACGCATTGCATCATCACCATGGAAACAGTTCCCA
  TCACCACCACCACCACCATCACCACCACCACCACCATGGACAGCAAGCCTTGGGTAACCGGACCAGGCCA
  AGGGTCTACAATTCTCCAACAAATAGCTCCTCTACCCAGGATTCTATGGAGGTTGGCCACAGTCACCACT
  CCATGACATCCCTGTCTTCCTCAACAACTTCTTCCTCGACATCTTCCTCCTCTACTGGTAATCAAGGCAA
  TCAGGCCTATCAGAACCGCCCAGTGGCTGCTAACACCTTGGACTTTGGACAGAATGGAGCTATGGACGTT
  AATTTGACCGTCTACTCCAATCCCCGCCAAGAGACTGGCATAGCTGGACATCCAACATACCAATTTTCTG
  CTAATACAGGTCCTGCACATTACATGACTGAAGGACATCTGGCGATGAGACAAGGGGCTGATAGAGAAGA
  GTCTCCCATGACAGGAGTTTGTGTGCAACAGAGTCCTGTAGCTAGCTCG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR221655 representing NM_007890
  Red=Cloning site Green=Tags(s)
  
  MHTGGETSACKPSSVRLAPSFSFHAAGLQMAAQMPHSHQYSDRRQPSISDQQVSALPYSDQIQQPLTNQV
  MPDIVMLQRRMPQTFRDPATAPLRKLSVDLIKTYKHINEVYYAKKKRRHQQGQGDDSSHKKERKVYNDGY
  DDDNYDYIVKNGEKWMDRYEIDSLIGKGSFGQVVKAYDRVEQEWVAIKIIKNKKAFLNQAQIEVRLLELM
  NKHDTEMKYYIVHLKRHFMFRNHLCLVFEMLSYNLYDLLRNTNFRGVSLNLTRKFAQQMCTALLFLATPE
  LSIIHCDLKPENILLCNPKRSAIKIVDFGSSCQLGQRIYQYIQSRFYRSPEVLLGMPYDLAIDMWSLGCI
  LVEMHTGEPLFSGANEVDQMNKIVEVLGIPPAHILDQAPKARKFFEKLPDGTWSLKKTKDGKREYKPPGT
  RKLHNILGVETGGPGGRRAGESGHTVADYLKFKDLILRMLDYDPKTRIQPYYALQHSFFKKTADEGTNTS
  NSVSTSPAMEQSQSSGTTSSTSSSSGGSSGTSNSGRARSDPTHQHRHSGGHFAAAVQAMDCETHSPQVRQ
  QFPAPLGWSGTEAPTQVTVETHPVQETTFHVAPQQNALHHHHGNSSHHHHHHHHHHHHHGQQALGNRTRP
  RVYNSPTNSSSTQDSMEVGHSHHSMTSLSSSTTSSSTSSSSTGNQGNQAYQNRPVAANTLDFGQNGAMDV
  NLTVYSNPRQETGIAGHPTYQFSANTGPAHYMTEGHLAMRQGADREESPMTGVCVQQSPVASS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007890
• ORF Size: 2289 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007890.2, NP_031916.1
• RefSeq Size: 6035 bp
• RefSeq ORF: 2292 bp
• Locus ID: 13548
• UniProt ID: Q61214
• Cytogenetics: 16 55.3 cM
• MW: 85.9 kDa
• Gene Summary: Dual-specificity kinase which possesses both serine/threonine and tyrosine kinase activities. May play a role in a signaling pathway regulating nuclear functions of cell proliferation. Modulates alternative splicing by phosphorylating the splice factor SRSF6 (By similarity). Exhibits a substrate preference for proline at position P+1 and arginine at position P-3. Has pro-survival function and negatively regulates the apoptotic process. Promotes cell survival upon genotoxic stress through phosphorylation of SIRT1. This in turn inhibits TP53 activity and apoptosis (PubMed:20167603).[UniProtKB/Swiss-Prot Function]