Kpna3 (NM_008466) Mouse Tagged ORF Clone

CAT#: MR208345

Kpna3 (Myc-DDK-tagged) - Mouse karyopherin (importin) alpha 3 (Kpna3)

Product Images

Circular map for MR208345

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Kpna3
• Synonyms: IPOA4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR208345 representing NM_008466
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGAGAACCCCGGCTTGGAGAACCACCGCATCAAGAGCTTCAAGAACAAGGGCCGCGATGTGGAGA
  CGATGCGAAGACACAGAAATGAAGTGACAGTTGAACTCCGGAAGAACAAAAGAGACGAACACTTACTGAA
  AAAGAGAAATGTTCCCCAAGAAGAAAGCTTAGAAGATTCAGATGTTGATGCTGATTTTAAAGCACAAAAT
  GTAACACTAGAAGCTATATTACAGAATGCCACAAGTGATAATCCTGTAGTCCAGTTGAGTGCTGTGCAGG
  CAGCAAGGAAACTATTATCCAGTGACAGAAATCCACCAATTGATGACCTAATAAAATCTGGGATTTTACC
  AATTCTAGTCAAATGTCTAGAAAGAGATGATAATCCTTCATTACAGTTTGAAGCTGCTTGGGCACTAACT
  AATATAGCATCGGGAACTTCTGCACAGACTCAAGCTGTTGTACAGTCTAATGCAGTACCACTTTTTCTGC
  GACTCCTCCATTCCCCACATCAGAATGTTTGTGAACAAGCGGTGTGGGCTTTGGGAAACATTATAGGTGA
  TGGTCCTCAATGTAGAGATTATGTCATATCACTGGGAGTTGTCAAACCTCTTCTGTCCTTCATCAATCCC
  TCCATTCCCATCACCTTCCTTCGGAACGTCACATGGGTCATTGTCAATCTCTGCAGGAATAAAGACCCCC
  CACCGCCTATGGAGACAGTTCAGGAGATTTTGCCAGCTTTGTGTGTGCTCATCTACCATACGGACATAAA
  TATTCTTGTAGACACTGTTTGGGCGCTGTCATACTTGACAGACGGGGGAAATGAGCAAATACAGATGGTT
  ATTGATTCAGGGGTTGTACCCTTCCTCGTACCCCTTCTGAGCCACCAGGAAGTCAAAGTTCAAACTGCAG
  CACTCAGAGCGGTTGGGAACATAGTGACTGGCACTGATGAGCAGACCCAGGTGGTTCTCAACTGTGATGT
  CCTGTCCCACTTCCCAAACCTCTTGTCACACCCAAAAGAGAAAATAAATAAGGAAGCAGTATGGTTCCTT
  TCCAATATAACAGCAGGCAATCAGCAGCAAGTTCAAGCTGTAATAGATGCTGGGTTAATCCCTATGATTA
  TTCATCAGCTCGCTAAGGGGGACTTTGGAACACAAAAGGAAGCTGCTTGGGCAATTAGCAACTTAACGAT
  AAGTGGCAGAAAAGATCAAGTTGAATACCTTGTACAGCAGAACGTAATACCACCATTCTGTAACTTACTG
  TCAGTAAAAGATTCTCAAGTGGTGCAGGTGGTTCTAGATGGTCTTAAAAACATCCTGATAATGGCTGGTG
  ATGAAGCAAGCACAATAGCTGAGATCATAGAAGAGTGTGGAGGTTTGGAGAAAATTGAAGTTTTGCAGCA
  ACATGAAAATGAAGACATATATAAATTAGCATTTGAAATCATAGATCAATATTTCTCTGGTGATGATATT
  GATGAAGATCCTAGCCTCATTCCAGAAGCAACACAAGGAGGTACTTACAATTTTGACCCAACAGCCAACC
  TTCAAACAAAAGAATTTAATTTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR208345 representing NM_008466
  Red=Cloning site Green=Tags(s)
  
  MAENPGLENHRIKSFKNKGRDVETMRRHRNEVTVELRKNKRDEHLLKKRNVPQEESLEDSDVDADFKAQN
  VTLEAILQNATSDNPVVQLSAVQAARKLLSSDRNPPIDDLIKSGILPILVKCLERDDNPSLQFEAAWALT
  NIASGTSAQTQAVVQSNAVPLFLRLLHSPHQNVCEQAVWALGNIIGDGPQCRDYVISLGVVKPLLSFINP
  SIPITFLRNVTWVIVNLCRNKDPPPPMETVQEILPALCVLIYHTDINILVDTVWALSYLTDGGNEQIQMV
  IDSGVVPFLVPLLSHQEVKVQTAALRAVGNIVTGTDEQTQVVLNCDVLSHFPNLLSHPKEKINKEAVWFL
  SNITAGNQQQVQAVIDAGLIPMIIHQLAKGDFGTQKEAAWAISNLTISGRKDQVEYLVQQNVIPPFCNLL
  SVKDSQVVQVVLDGLKNILIMAGDEASTIAEIIEECGGLEKIEVLQQHENEDIYKLAFEIIDQYFSGDDI
  DEDPSLIPEATQGGTYNFDPTANLQTKEFNF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_008466
• ORF Size: 1563 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_008466.5, NP_032492.1
• RefSeq Size: 4167 bp
• RefSeq ORF: 1566 bp
• Locus ID: 16648
• UniProt ID: O35344
• Cytogenetics: 14 D1
• MW: 57.8 kDa
• Gene Summary: Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. In vitro, mediates the nuclear import of human cytomegalovirus UL84 by recognizing a non-classical NLS.[UniProtKB/Swiss-Prot Function]