Palb2 (BC066140) Mouse Tagged ORF Clone

CAT#: MG210399

Palb2 (tGFP-tagged) - Mouse partner and localizer of BRCA2 (cDNA clone MGC:76443 IMAGE:30459312)

Product Images

Circular map for MG210399

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Palb2
• Synonyms: 4732427B05
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG210399 representing BC066140
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAGAGCTTTCCGGGAAGCCCCTCAGCTATGCGGAGAAGGAAAAGTTGAAGGAAAAGCTAGCATTTT
  TGAAAAAGGAATACAGCAGGACACTTGCTCGACTTCAGCGTGCCAAAAGAGCTGAGAAGGCTAAGAACTC
  TAAGAAAGCCATAGAAGATGGTGTGCCCCAGCCAGAAGCTTCCTCACAGCTAAGCCACTCTGAATCTATA
  AATAAAGGCTTTCCTTGTGACACATTGCAAAGCAACCATCTGGATGAGGAGACTGGAGAAAACATCTCTC
  AGATACTTGATGTTGAACCTCAATCCTTTAACTGTAAACAAGGCAAAGAAGTATTACATACACCAAGAGC
  AGGTGACATTCAAGGACAACTTTTGCATAGCACCAGCAGCCCTGATGGCAAGAAAGAGCAGAACACGCTT
  CCGGGGACAACAAAGACGCCGTGGGAGAAGTCATCTGTTTCACAGGAGAAGGAAGATTATTTTGACACTA
  ATTCTCTGGCGCTCCTTGACTTTGAGTTACCTGATGAAGACTTTGGGCTTCTTAAACTTGAAAAATTGAA
  GTCCTGCTCGGAAAAACTGATTGAGTCTCCTGACTCAAAAAACTGTGGTGAGAGGCTTCCTAGAGAAGGA
  AACCATGCTGCTCTGGAGGAACTGCAAAGAGATTCGGAGACGGAGGGCTTGGAAGAGGAGCTCACTGTTC
  CACCAGGAGAAGCGTACCGTCCAGGGCCAACCCTGAGAAGGCAGCCAGGGAGCAAGGACCTTTCTTCATC
  CATAGTGCTTTTCACTCCTGCAGACACTGCTGCGCCTAACGACAGTGGCAGGCCTCCTCCCTCCCTGTGC
  TCACCTGCTTTCCCCATCTTAGGCATGACTCCAGCTCTTGGCTCCCAAGCAGCCGGTGAGACTCTATCTA
  CTGAAGCTGCACAGCCTTGCTCTACATCCCAACCTCCTCTCTTGGGAGACACAAACAGTCTTGTCAATAA
  CAGTAAACAGTGCAACAGTTCAGCCTGCTCACCAAAACCGGACACCAACCTGCAAGCGTCAGGTAGGCAA
  GGACAACCTGCCTGTGACAGTGACTCTGGCCCCCAAGCAACACCTCTACCTGTTGAGTCATTCACTTTCA
  GAGAAAATCAGCTTTGTGGAAATGCATGCCTCGAGTTACATGAACATTCCACTGAACAGACTGAAACTGC
  AGATCGCCCTGCTTGTGACAATCTAAACCCAGGAAACCTACAGTTGGTTTCAGAGTTAAAGAATCCTTCC
  AGTTCCTGCTCTGTGGATGTGAGCGCCATGTGGTGGGAAAGAGCTGGTGCTAAGGAGCCATGTATCGTAA
  CTGCTTGTGAAGATGTAGTTTCTCTTTGGAAACCCTTGAATTCTCTGCAGTGGGAGAAAGTTCATACCTG
  GCACTTCACAGAGGTTCCAGTATTACAAATAGTTCCAGTGCCTGATGTTTACAATCTTATATGTGTAGCT
  TTGGGAAGTTTGGAAATCAGAGAAATCAGGGCGTTGCTGTGCTCTTCTGGTGATGACAGTGAAAAGCAAG
  TCCTCCTGAAATCTGGAGATATAAAAGCTATGCTTGGCCTGACAAAGCGGAGGCTAGTTAGTAGCACTGG
  GACCTTTTGCAATCAACAAATACAAATCATGACATTTGCTGACGACGGAAGCAGCAAAGATGAGCAGCTT
  TTGATGCCTCCTGATGAGACTGTACTGACTTTTGCTGAAGTCCAAGGGACGCAGGAAGCTCTGCTTGGTA
  CTACCACTGTGAACAGCATTGTGATCTGGAATTTAAAAACCGGCCAGCTCCTGAAGAAGATGCACATTGA
  TGACTCCTACCAGGCTTCGGTCTGTCACGGAGCCTATTCTGAGAAGGGGCTCCTGTTTGTTGTTGTGAGT
  CAACCTTGTGCCAAAGAGAGCCAGGCCCTTGGAAGCCCTGTGTTCCAGCTGCTGGTGATTAACCCTAAGA
  CGGCCCAGAGTGTGGGTGTTCTGCTGTGTTCCCTTCCTCAGGGGCAGGCTGGAAGGTTCCTGGAAGGGGA
  TGTGAAAGATCATGTCGCAGCAGCAGTCCTGACTTCTGGGACAATTGCCATTTGGGATTTGCTTCTGGGT
  CACTGCACGGCTCTCCTCCCACCTGTCTCTGACCAGAGTTGGTCTTTGGTTAAATGGTCGGGTACAGATT
  CTCACTTGCTAGCTGGACAAAAGGATGGAAATATATTTATATACCGATACTTT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG210399 representing BC066140
  Red=Cloning site Green=Tags(s)
  
  MEELSGKPLSYAEKEKLKEKLAFLKKEYSRTLARLQRAKRAEKAKNSKKAIEDGVPQPEASSQLSHSESI
  NKGFPCDTLQSNHLDEETGENISQILDVEPQSFNCKQGKEVLHTPRAGDIQGQLLHSTSSPDGKKEQNTL
  PGTTKTPWEKSSVSQEKEDYFDTNSLALLDFELPDEDFGLLKLEKLKSCSEKLIESPDSKNCGERLPREG
  NHAALEELQRDSETEGLEEELTVPPGEAYRPGPTLRRQPGSKDLSSSIVLFTPADTAAPNDSGRPPPSLC
  SPAFPILGMTPALGSQAAGETLSTEAAQPCSTSQPPLLGDTNSLVNNSKQCNSSACSPKPDTNLQASGRQ
  GQPACDSDSGPQATPLPVESFTFRENQLCGNACLELHEHSTEQTETADRPACDNLNPGNLQLVSELKNPS
  SSCSVDVSAMWWERAGAKEPCIVTACEDVVSLWKPLNSLQWEKVHTWHFTEVPVLQIVPVPDVYNLICVA
  LGSLEIREIRALLCSSGDDSEKQVLLKSGDIKAMLGLTKRRLVSSTGTFCNQQIQIMTFADDGSSKDEQL
  LMPPDETVLTFAEVQGTQEALLGTTTVNSIVIWNLKTGQLLKKMHIDDSYQASVCHGAYSEKGLLFVVVS
  QPCAKESQALGSPVFQLLVINPKTAQSVGVLLCSLPQGQAGRFLEGDVKDHVAAAVLTSGTIAIWDLLLG
  HCTALLPPVSDQSWSLVKWSGTDSHLLAGQKDGNIFIYRYF
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC066140
• ORF Size: 2225 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC066140, AAH66140
• RefSeq Size: 2568 bp
• RefSeq ORF: 2225 bp
• Locus ID: 233826
• Cytogenetics: 7 F2
• Gene Summary: Plays a critical role in homologous recombination repair (HRR) through its ability to recruit BRCA2 and RAD51 to DNA breaks. Strongly stimulates the DNA strand-invasion activity of RAD51, stabilizes the nucleoprotein filament against a disruptive BRC3-BRC4 polypeptide and helps RAD51 to overcome the suppressive effect of replication protein A (RPA). Functionally cooperates with RAD51AP1 in promoting of D-loop formation by RAD51. Serves as the molecular scaffold in the formation of the BRCA1-PALB2-BRCA2 complex which is essential for homologous recombination. Via its WD repeats is proposed to scaffold a HR complex containing RAD51C and BRCA2 which is thought to play a role in HR-mediated DNA repair. Essential partner of BRCA2 that promotes the localization and stability of BRCA2. Also enables its recombinational repair and checkpoint functions of BRCA2. May act by promoting stable association of BRCA2 with nuclear structures, allowing BRCA2 to escape the effects of proteasome-mediated degradation. Binds DNA with high affinity for D loop, which comprises single-stranded, double-stranded and branched DNA structures. May play a role in the extension step after strand invasion at replication-dependent DNA double-strand breaks; together with BRCA2 is involved in both POLH localization at collapsed replication forks and DNA polymerization activity (By similarity).[UniProtKB/Swiss-Prot Function]