Human EDC3 (NM_001142443) AAV Particle

CAT#: RC226857A1V

AAV ORF Particles, serotype AAV-2, EDC3 (Myc-DDK-tagged)-Human enhancer of mRNA decapping 3 homolog (S. cerevisiae) (EDC3), transcript variant 1, 250ul, >10^13 GC/mL

Specifications

Product Data

• Tag: Myc-DDK
• Symbol: EDC3
• Synonyms: hYjeF_N2-15q23; LSM16; MRT50; YJDC; YJEFN2
• Vector: pAAV-AC-Myc-DDK
• Mammalian Cell Selection: None
• Sequence Data:
  ORF Nucleotide Sequence

  >RC226857 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTACAGATTGGCTGGGAAGTATTGTGTCCATCAATTGTGGAGATAGCTTGGGTGTCTATCAGGGAA
  GAGTGTCAGCTGTGGATCAGGTCAGCCAGACCATTTCTCTCACCCGGCCTTTCCATAATGGAGTGAAGTG
  TCTTGTTCCAGAAGTCACCTTCAGGGCAGGTGACATTACGGAGTTAAAAATTCTGGAGATACCAGGACCT
  GGAGACAACCAACATTTTGGAGACCTTCATCAAACAGAATTAGGCCCCTCTGGTGCTGGCTGCCAAGTGG
  GCATCAATCAGAATGGCACAGGCAAGTTTGTCAAGAAGCCAGCCTCTTCCAGCAGTGCCCCTCAGAATAT
  CCCTAAGAGGACAGATGTGAAGAGCCAGGATGTTGCCGTTTCCCCGCAGCAGCAACAGTGCTCAAAGAGC
  TATGTCGACAGGCACATGGAATCCTTGAGTCAGTCCAAAAGTTTCCGTCGTCGGCACAACTCCTGGTCAT
  CTAGTAGCAGGCACCCAAATCAGGCAACTCCCAAGAAAAGTGGTTTAAAGAATGGCCAGATGAAGAATAA
  AGATGACGAGTGCTTCGGGGATGATATTGAGGAGATCCCAGACACAGATTTTGATTTTGAAGGGAACCTG
  GCTCTTTTTGACAAGGCAGCTGTGTTTGAGGAGATTGATACCTATGAAAGGAGAAGTGGTACCCGTTCCC
  GGGGCATCCCAAATGAAAGGCCCACTCGGTACCGCCATGATGAGAACATCTTGGAGTCCGAGCCCATTGT
  CTATCGACGGATCATAGTGCCCCACAACGTGAGCAAGGAGTTCTGCACGGACTCTGGCCTGGTTGTCCCA
  AGTATTTCCTATGAGCTGCATAAAAAGCTGTTGTCCGTGGCTGAGAAGCATGGGCTGACCCTTGAGCGGA
  GACTGGAGATGACAGGTGTGTGTGCCAGTCAGATGGCACTGACCCTCCTCGGAGGACCTAACAGGTTGAA
  TCCCAAAAATGTTCACCAGAGGCCTACAGTGGCTCTACTGTGTGGACCTCATGTGAAGGGGGCTCAGGGT
  ATCAGCTGTGGAAGGCACCTAGCCAACCATGATGTCCAGGTCATCCTTTTCCTGCCCAATTTTGTCAAGA
  TGTTGGAATCTATCACCAATGAGCTGTCGCTCTTCAGCAAGACCCAAGGCCAACAAGTGTCTAGCCTCAA
  AGATCTGCCCACTAGCCCTGTGGACCTGGTCATCAACTGCCTGGATTGCCCTGAGAACGTCTTCCTGCGC
  GATCAACCCTGGTACAAGGCAGCTGTGGCCTGGGCCAACCAGAACCGGGCACCAGTACTCAGCATAGACC
  CTCCTGTGCATGAAGTCGAACAGGGCATTGATGCCAAATGGTCACTGGCACTGGGCCTGCCTCTGCCACT
  GGGGGAGCACGCAGGCCGTATCTATTTGTGCGACATTGGCATTCCCCAGCAGGTCTTCCAGGAGGTGGGC
  ATCAACTACCACTCGCCCTTTGGCTGCAAGTTTGTTATCCCACTGCACTCTGCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC226857 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MATDWLGSIVSINCGDSLGVYQGRVSAVDQVSQTISLTRPFHNGVKCLVPEVTFRAGDITELKILEIPGP
  GDNQHFGDLHQTELGPSGAGCQVGINQNGTGKFVKKPASSSSAPQNIPKRTDVKSQDVAVSPQQQQCSKS
  YVDRHMESLSQSKSFRRRHNSWSSSSRHPNQATPKKSGLKNGQMKNKDDECFGDDIEEIPDTDFDFEGNL
  ALFDKAAVFEEIDTYERRSGTRSRGIPNERPTRYRHDENILESEPIVYRRIIVPHNVSKEFCTDSGLVVP
  SISYELHKKLLSVAEKHGLTLERRLEMTGVCASQMALTLLGGPNRLNPKNVHQRPTVALLCGPHVKGAQG
  ISCGRHLANHDVQVILFLPNFVKMLESITNELSLFSKTQGQQVSSLKDLPTSPVDLVINCLDCPENVFLR
  DQPWYKAAVAWANQNRAPVLSIDPPVHEVEQGIDAKWSLALGLPLPLGEHAGRIYLCDIGIPQQVFQEVG
  INYHSPFGCKFVIPLHSA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Species: Human
• Serotype: AAV-2
• ACCN: NM_001142443
• ORF Size: 1524 bp
• Storage Buffer: PBS with 0.001% Pluronic F68
• Stability: AAV is stable for 1 year when stored at -80°C (long-term storage) or 2-3 weeks when stored at -20°C (short-term storage). Thaw the vial of AAV on ice prior to use and keep it on ice during the experiment. Thawed AAV can be stored at 4°C for 1-2 weeks. Whenever possible, particles should be aliquoted into single use portions to avoid repeated freeze/thaw cycles. Please aliquot at least 10ul per tube and use low protein binding tubes to avoid loss of virus.

Reference Data

• RefSeq: NM_001142443.1
• RefSeq Size: 4086 bp
• RefSeq ORF: 1527 bp
• Locus ID: 80153
• UniProt ID: Q96F86
• Cytogenetics: 15q24.1
• MW: 56.1 kDa