Human TIMP-4 ELISA Kit

CAT#: EA100364

Human TIMP-4 ELISA Kit

Specifications

Product Data

• Assay Type: Sandwich ELISA kit of Quantitative Detection for Human TIMP-4
• Assay Length: 3.5 hours incubations; 1 hour washing and analyzing samples
• Signal: Colorimetric
• Curve Range: 78pg/ml-5000pg/ml
• Sample Type: Cell culture supernates, serum, plasma(heparin, EDTA) and human milk.
• Sample Volume: 100 ul
• Specificity: This kit is used for quantitative detection of human TIMP-4
• Sensitivity: <10pg/ml
• Reactivities: Human
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Interference: No significant interference observed with available related molecules.
• Components:
  • 96-well plate precoated with anti- human TIMP-4 antibody 1
  • Lyophilized recombinant human TIMP-4 standard 10ng/tubex2
  • Biotinylated anti- human TIMP-4 antibody 130ul(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100)
  • Sample diluent buffer 30 ml
  • Antibody diluent buffer 12ml
  • ABC diluent buffer 12ml
  • TMB color developing agent 10ml
  • TMB stop solution 10ml
• Background: Tissue inhibitor of metalloproteinases 4, also called Metalloproteinase inhibitor 4, is an enzyme that in humans is encoded by the TIMP4 gene. This gene belongs to the TIMP gene family. The proteins encoded by this gene family are inhibitors of the matrix metalloproteinases, a group of peptidases involved in degradation of the extracellular matrix. This gene is mapped to 3p25.2. The secreted, netrin domain-containing protein encoded by this gene is involved in regulation of platelet aggregation and recruitment and may play role in hormonal regulation and endometrial tissue remodeling.
• Gene Symbol: TIMP4
• Gene ID: 7079