Hamartin (TSC1) Rabbit Polyclonal Antibody

CAT#: TA590017

Rabbit Polyclonal TSC1 Antibody

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TSC1 (RC213332, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TSC1. Positive lysates LY400132 (100ug) and LC400132 (20ug) can be purchased separately from OriGene.

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  ELISA: TSC1 Antibody

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution: WB 1:20000,ELISA 1:100-1:2000
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: DNA immunization. This antibody is specific for the Middle Region of the target protein.
• Formulation: 20 mM Potassium Phosphate, 150 mM Sodium Chloride, pH 7.0
• Concentration: 0.77mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: TSC complex subunit 1
• Database Link:
  NP_000359
  Entrez Gene 7248 Human
  UniProt ID Q92574
• Background: Tuberous sclerosis complexes-1 (TCS1 also known as Hamartin) is a tumor suppressor that form a complex with TSC2 (Tuberin. This complex is known to control various cellular functions including cell cycle, endocytosis, adhesion, and transcription (1). The TSC1/TSC2 complex inhibits phosphorylation of S6kinase and 4E-BP1 through inactivation of mTOR (2). Recently it has been shown that TSC1/Hamartin is localized to the centrosome and negatively regulates Plk1 implicating TSC1/hamartin to the regulation of centrosome duplication (3). Tuberous sclerosis (TSC), an autosomal dominant disorder that affects 1 in 6000 individuals, is caused by a mutation in either the TSC1 or TSC2 tumor suppressor gene.
• Synonyms: LAM; TSC
• Note: This antibody was generated by SDIX's Genomic Antibody Technology ® (GAT). Learn about GAT

Reference Data

• Protein Pathways: Insulin signaling pathway, mTOR signaling pathway