HERPUD1 Mouse Monoclonal Antibody [Clone ID: OTI1A2]

CAT#: TA507019

Purified HERPUD1 mouse monoclonal antibody, clone OTI1A2 (formerly 1A2)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY HERPUD1 (Cat# RC200693, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HERPUD1(Cat# TA507019). Positive lysates LY423260 (100ug) and LC423260 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-HERPUD1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-HERPUD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA507019)

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-HERPUD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA507019)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-HERPUD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA507019)

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  Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-HERPUD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA507019)

• Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-HERPUD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA507019)

• Anti-HERPUD1 mouse monoclonal antibody (TA507019) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY HERPUD1 (RC200693).

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Specifications

Product Data

• Clone Name: OTI1A2
• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:400~4000, IHC 1:150, IF 1:100
• Reactivities: Human, Rat, Mouse
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human HERPUD1(NP_001010989) produced in HEK293T cell.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 43.4 kDa
• Gene Name: homocysteine inducible ER protein with ubiquitin like domain 1
• Database Link:
  NP_001010989
  Entrez Gene 85430 RatEntrez Gene 9709 Human
  UniProt ID Q15011
• Background: The accumulation of unfolded proteins in the endoplasmic reticulum (ER) triggers the ER stress response. This response includes the inhibition of translation to prevent further accumulation of unfolded proteins, the increased expression of proteins involved in polypeptide folding, known as the unfolded protein response (UPR), and the destruction of misfolded proteins by the ER-associated protein degradation (ERAD) system. This gene may play a role in both UPR and ERAD. Its expression is induced by UPR and it has an ER stress response element in its promoter region while the encoded protein has an N-terminal ubiquitin-like domain which may interact with the ERAD system. This protein has been shown to interact with presenilin proteins and to increase the level of amyloid-beta protein following its overexpression. Alternative splicing of this gene produces multiple transcript variants encoding different isoforms. The full-length nature of all transcript variants has not been determined. [provided by RefSeq, Jan 2013]
• Synonyms: HERP; Mif1; SUP

Reference Data

• Protein Families: Druggable Genome