WDR68 (DCAF7) Rabbit Polyclonal Antibody

CAT#: TA375343

DCAF7 Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using DCAF7 antibody (TA375343) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 10s.

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  Immunofluorescence analysis of NIH/3T3 cells using DCAF7 Rabbit pAb (TA375343) at dilution of 1:150 (40x lens). Blue: DAPI for nuclear staining.

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  Immunofluorescence analysis of PC-12 cells using DCAF7 Rabbit pAb (TA375343) at dilution of 1:150 (40x lens). Blue: DAPI for nuclear staining.

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  Immunofluorescence analysis of U2OS cells using DCAF7 Rabbit pAb (TA375343) at dilution of 1:150 (40x lens). Blue: DAPI for nuclear staining.

Specifications

Product Data

• Applications: ICC/IF, IHC, WB
• Recommended Dilution: WB,1:500 - 1:2000
  IHC,1:50 - 1:200
  IF,1:10 - 1:100
• Reactivities: Human, Mouse, Rat
• Modifications: Unmodified
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-342 of human DCAF7 (NP_005819.3).
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 16kDa/38kDa
• Gene Name: DDB1 and CUL4 associated factor 7
• Database Link:
  Entrez Gene 10238 Human
  UniProt ID P61962
• Background: This gene encodes a protein with multiple WD40 repeats which facilitate protein-protein interactions and thereby enable the assembly of multiprotein complexes. This protein has been shown to function as a scaffold protein for protein complexes involved in kinase signaling. This highly conserved gene is present in eukaryotic plants, fungi, and animals. The ortholog of this gene was first identified in plants as a key regulator of anthocyanin biosynthesis and flower pigmentation. Alternative splicing results in multiple transcript variants.
• Synonyms: AN11; HAN11; SWAN-1; WDR68