Bile Acid Receptor (NR1H4) Rabbit Polyclonal Antibody

CAT#: TA309773

Rabbit Polyclonal FXR Antibody

Product Images

Immunocytochemistry/Immunofluorescence: FXR/NR1H4 Antibody TA309773 - A549 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-FXR/NR1H4 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

• 

  Immunocytochemistry/Immunofluorescence: FXR/NR1H4 Antibody TA309773 - NIH3T3 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-FXR/NR1H4 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

• 

  Western Blot: FXR Antibody TA309773 - Detection of FXR in HeLa nuclear extract using TA309773. ECL 30 minute detection.

• 

  Western Blot: FXR/NR1H4 Antibody TA309773 - Total protein from human A549, U2OS cell and mouse 3T3 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-FXR (TA309773) in block buffer and detected with an anti-rabbit HRP secondary antibody using NovaLume chemiluminescence detection reagent (NPB2-61915).

Specifications

Product Data

• Applications: ICC/IF, WB
• Recommended Dilution: Western Blot: 1 - 4 ug/mL, Immunocytochemistry/ Immunofluorescence: 1:100
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: A synthetic peptide made to the C-terminus of the human FXR protein.
• Formulation: Tris-glycine with 150 mM NaCl and 0.05% Sodium Azide
• Concentration: lot specific
• Purification: Immunogen affinity purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: nuclear receptor subfamily 1 group H member 4
• Database Link:
  NP_005114
  Entrez Gene 9971 Human
  UniProt ID Q96RI1
• Synonyms: BAR; FXR; HRR-1; HRR1; PFIC5; RIP14

Reference Data

• Protein Families: Druggable Genome, Nuclear Hormone Receptor, Transcription Factors