Creatine kinase B type (CKB) Rabbit Polyclonal Antibody

CAT#: TA308722

Rabbit Polyclonal antibody to Creatine kinase (brain) (creatine kinase, brain)

Product Images

Sample (50 ug of whole cell lysate). A: Mouse brain. 10% SDS PAGE. TA308722 diluted at 1:10000.

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  Sample (30 ug of whole cell lysate). A:H1299. B:HeLa S3. 12% SDS PAGE. TA308722 diluted at 1:1000

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  Creatine kinase (brain) antibody detects Creatine kinase protein by western blot analysis. Rat tissue extracts (50 ug) was separated by 10 % SDS-PAGE, and blotted with Creatine kinase (brain) antibody (TA308722) diluted by 1:1000

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  Immunohistochemical analysis of paraffin-embedded HSC3 xenograft, using Creatine kinase (brain) (TA308722) antibody at 1:100 dilution.

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  Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Creatine Kinase BB (TA308722) antibody at 1:100 dilution.

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Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: ICC/IF:1:100-1:1000; IHC:1:100-1:1000; WB:1:500-1:10000
• Reactivities: Human, Mouse (Predicted: Bovine, Chicken, Pig, Rabbit, Xenopus, X. tropicalis)
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fragment corresponding to a region within amino acids 165 and 381 of Creatine kinase (brain) (Uniprot ID#P12277)
• Formulation: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Concentration: lot specific
• Purification: Purified by antigen-affinity chromatography.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 43 kDa
• Gene Name: creatine kinase B
• Database Link:
  NP_001814
  Entrez Gene 12709 MouseEntrez Gene 1152 Human
  UniProt ID P12277
• Background: The protein encoded by this gene is a cytoplasmic enzyme involved in energy homeostasis. The encoded protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in brain as well as in other tissues, and as a heterodimer with a similar muscle isozyme in heart. The encoded protein is a member of the ATP:guanido phosphotransferase protein family. A pseudogene of this gene has been characterized. [provided by RefSeq]
• Synonyms: B-CK; BCK; CKBB; HEL-211; HEL-S-29
• Note: Seq homology of immunogen across species: Chicken (97%), Pig (96%), Rabbit (96%), Xenopus laevis (88%), Bovine (96%), Xenopus tropicalis (88%)

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Arginine and proline metabolism, Metabolic pathways