gdh Goat Polyclonal Antibody

CAT#: R1082

gdh goat polyclonal antibody, Serum

Product Images

Goat anti Glucose Dehydrogenase antibody. was used to detect purified Glucose Dehydrogenase under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified target proteins contained 4% BME and were boiled for 5 minutes. Samples of ~1ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1/3,000 dilution of primary antibody. Detection shown was using Dylight 488 conjugated secondary antibody. Images were collected using the BioRad VersaDoc System.

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution: Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoprecipitation and most immunological methods requiring high titer and specificity.
  Recommended Dilutions: This product has been assayed against 1.0 ug of Glucose Dehydrogenase [Bacillus] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Goat) and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:7,500 to 1:32,000 of the reconstitution concentration is suggested for this product.
• Reactivities: Bacillus sp.
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Glucose Dehydrogenase [Bacillus].
• Specificity: Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Glucose Dehydrogenase [Bacillus].
  Cross reactivity against Glucose Dehydrogenase from other sources is unknown.
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01% sodium azide as preservative.
  State: Serum
  State: Lyophilized purified Ig fraction.
• Reconstitution Method: Restore with 2.0 ml of deionized water (or equivalent).
• Concentration: lot specific
• Purification: Prepared from monospecific antiserum by a delipidation and defibrination.
• Conjugation: Unconjugated
• Storage: Store vial at 2-8°C prior to restoration. Centrifuge product if not completely clear after standing at room temperature. For extended storage aliquot contents and freeze at -20°C or below.
  This product is stable for one month at 2-8°C as an undiluted liquid.
  Dilute only prior to immediate use.
  Avoid cycles of freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Database Link:
  UniProt ID P07999
• Background: Glucose Dehydrogenase catalyses the oxidation of D glucose without prior phosphorylation to D beta gluconolactone using NAD or NADP as a coenzyme. The enzyme is a tetrameric protein, each of the 4 identical subunits containing 262 amino acid residues. This family is a subset of a more general family of short chain dehydrogenases and reductases.
• Synonyms: Glucose 1-dehydrogenase, gdh, BSU03930