CA2 Rabbit Polyclonal Antibody

CAT#: R1069BS

CA2 rabbit polyclonal antibody, Biotin, Purified

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution: Western blot: 1/2,000-1/10,000
  ELISA: 1/20,000-1/100,000
  This product has been assayed against 1.0 µg of Carbonic anhydrase 2 from bovine erythrocytes in a standard capture ELISA using peroxidase conjugated streptavidin and ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1/4,000 to 1/20,000 of the reconstitution concentration is suggested.
• Reactivities: Bovine, Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Carbonic anhydrase 2 from bovine erythrocytes
• Specificity: This antibody detects Carbonic anhydrase 2.
  Immunoelectrophoresis give a single precipitin arc against anti-biotin, anti-rabbit serum as well as purified and partially purified Carbonic anhydrase 2 [bovine erythrocytes].
• Formulation: 0.02 M Potassium phosphate, 0.15 M Sodium chloride, pH 7.2
  Label: Biotin
  State: Purified
  State: Lyophilized Ig fraction
  Stabilizer: 10 mg/ml BSA (immunoglobulin and protease free)
  Preservative: 0.01% (w/v) Sodium azide
• Reconstitution Method: Restore with 0.1 ml of deionized water (or equivalent).
• Concentration: lot specific
• Purification: Delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
• Conjugation: Biotin
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 280740 Bovine
  UniProt ID P00921
• Background: Carbonic anhydrase II (CAII) is a single polypeptide chain of molecular weight 29kDa. It is present in the cytosol of most tissues, but highest concentrations are found, like Carbonic Anhydrase I, in erythrocytes. The concentration in erythrocytes is about 20µM. It catalyses the hydration of CO2 and the hydrolysis of esters. Zn2+ ion cofactor is required for enzyme activity. Certain point mutations occur without apparent clinical effect, however, complete absence leads to mild mental retardation and cerebral calcification, osteoporosis and renal tubular acidosis.
• Synonyms: Carbonic anhydrase II, Carbonate dehydratase II, Carbonic anhydrase C, CA2, CA-II, CAC