Androgen Receptor (AR) Mouse Monoclonal Antibody [Clone ID: AR441]

CAT#: DM245-05

Androgen Receptor (AR) mouse monoclonal antibody, clone AR441, Purified

Product Images

Formalin fixed paraffin embedded human prostate stained with Androgen Receptor antibody DM245

Specifications

Product Data

• Clone Name: AR441
• Applications: IHC, WB
• Recommended Dilution: Western Blot: 1/50-1/100.
  Immunohistochemistry on Paraffin Embedded Sections: Use at 1/100-1/200 with Polymer Detection system.
  Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM Citrate buffer, pH 6.0 or 1 mM EDTA, pH 8.0 buffer prior to immunostaining.
  Incubation Time: 30 minutes at RT.
  Recommended Positive Control: Prostate carcinoma.
• Reactivities: Canine, Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: BALB/C mice were injected with a synthetic peptide, amino acid 340-356 of Human Androgen Receptor
• Specificity: This antibody is specific to a protein of 110 kD, identified as androgen receptor. This antibody reacts with full length of AR and also with the newly described A form of the receptor.
  This antibody does not cross react with estrogen, progesterone or glucocorticoid receptors.
  Cellular Localization: Nuclear.
• Formulation: State: Purified
  State: Liquid purified Ig fraction
  Preservative: Less than 0.1% Sodium Azide
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 110 kDa
• Gene Name: androgen receptor
• Database Link:
  Entrez Gene 367 Human
  UniProt ID P10275
• Background: The androgen receptor (AR) is a 110 kDa androgen-dependent transcription factor that is a member of the steroid/nuclear receptor gene superfamily. The AR signaling pathway plays a key role in development and function of male reproductive organs, including the prostate and epididymis. AR also plays a role in nonreproductive organs, such as muscle, hair follicles, and brain. Abnormalities in the AR signaling pathway have been linked to a number of diseases, including prostate cancer, Kennedy's disease and male infertility. The PI3K/Akt signaling pathway plays an important role in regulating AR activity through phosphorylation of AR at Ser213/210 and Ser791/790. Growth factors or cytokines may induce phosphorylation of AR through the PI3K/Akt pathway. Activation of the PI3K/AKt pathway is thought to have a survival role in prostate cancer by protecting cells from apoptosis.
• Synonyms: Dihydrotestosterone receptor, DHTR, NR3C4