Cd4 Rat Monoclonal Antibody [Clone ID: CT-CD4]

CAT#: CL004FX

Cd4 rat monoclonal antibody, clone CT-CD4, FITC

Product Images

Cell Source: CD3e Positive Spleen Cells Percentage of cells stained above control: 52.4%

Specifications

Product Data

• Clone Name: CT-CD4
• Applications: FC
• Recommended Dilution: Flow Cytometry (see Protocols).
  
  
• Reactivities: Mouse
• Host: Rat
• Isotype: IgG2a
• Clonality: Monoclonal
• Specificity: This CT-CD4 monolconal antibody (mAb) recognizes mouse CD4.
• Formulation: PBS, 0.1% NaN3 and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/ml.
  Label: FITC
  State: Liquid purified IgG
• Concentration: lot specific
• Conjugation: FITC
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: CD4 antigen
• Database Link:
  Entrez Gene 12504 Mouse
  UniProt ID P06332
• Background: CD4 (L3T4) which is expressed on the majority of thymocytes and on the MHC class II restricted subset of mature T cells including Th cells1,2. Mouse CD4 has also been reported to be present on multipotential hematopoietic stem cells, bone marrow myeloid precursors, and intrathymic precursors2,3. As a coreceptor in the TCR complex, CD4 is involved in T cell activation through interaction with MHC class II on APC’s and in signal transduction via protein tyrosine kinase lck1.
• Synonyms: T-cell surface antigen T4/Leu-3
• Note: Protocol: FLOW CYTOMETRY ANALYSIS:
  1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cell population with Lympholyte®-M cell separation medium.
  2. Wash 2 times.
  3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50 µl of this suspension to each tube (each tube will then contain 1x10e6 cells, representing 1 test).
  4. To each tube, add ~1.0 µg* of this Ab per 1x10e6 cells.
  5. Vortex the tubes to ensure thorough mixing of antibody and cells.
  6. Incubate the tubes for 30 minutes at 4°C. (It is recommended that the tubes be protected from light, since most fluorochromes are light sensitive.)
  7. Wash 2 times at 4°C.
  8. Resuspend the cell pellet in 50 µl ice cold media B.
  9. Transfer to suitable tubes for flow cytometric analysis containing 15 µl of propidium iodide at 0.5 mg/ml in PBS. This stains dead cells by intercalating in DNA.
  
  MEDIA:
  A. Phosphate buffered saline (pH 7.2) + 5% normal serum of host species + sodium azide (100 µl of 2M sodium azide in 100 mls).
  B. Phosphate buffered saline (pH 7.2) + 0.5% Bovine serum albumin + sodium azide (100 µl of 2M sodium azide in 100 mls).
  
  Tissue Distribution by Flow Cytometry Analysis:
  Mouse Strain: C57BL/6
  Cell Concentration: 1x10e6 cells per test
  Antibody Concentration Used: 1.0 µg/10e6 cells
  Isotypic Control: FITC Rat IgG2a