Neutrophil Elastase (ELANE) Mouse Monoclonal Antibody [Clone ID: 39A]

CAT#: BM382

Neutrophil Elastase (ELANE) mouse monoclonal antibody, clone 39A, Purified

Specifications

Product Data

• Clone Name: 39A
• Applications: ELISA, FC, FN
• Recommended Dilution: ELISA.
  Flow Cytometry.
  Functional Assays.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Elastase from Human Neutrophils.
• Specificity: This antibody is specific for Neutrophil Elastase.
• Formulation: PBS, pH 7.2
  State: Purified
  State: Liquid purified IgG fraction
  Preservative: 0.09% Sodium Azide
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein G
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: elastase, neutrophil expressed
• Database Link:
  Entrez Gene 1991 Human
  UniProt ID P08246
• Background: Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Elastase is a serine protease which exhibits endopeptidase activity on many proteins, its principal substrate, however, is elastin and it is unique among proteases in its ability to hydrolyse native elastin. It is present in the pancreas, leukocytes and serum. Elastase consists of a single polypeptide chain with a molecular weight of 29.5kDa. Humans have six elastase genes which encode the structurally similar proteins elastase 1, 2, 2A, 2B, 3A, and 3B. Like most of the human elastases, elastase 2A is secreted from the pancreas as a zymogen. In other species, elastase 2A has been shown to preferentially cleave proteins after leucine, methionine, and phenylalanine residues. Elastase 1 is not a pancreatic enzyme: clinical literature that describes human elastase 1 activity in the pancreas is actually referring to elastase 2A.
• Synonyms: ELANE, ELA2, Neutrophil elastase, Elastase-2, PMN elastase, Bone marrow serine protease, Medullasin, Human leukocyte elastase, HLE