Creatine kinase B type (CKB) Rabbit Polyclonal Antibody

CAT#: AP13618PU-N

Creatine kinase B type (CKB) rabbit polyclonal antibody, Purified

Product Images

The anti-CKB Pab is used in Western blot to detect CKB in Y79 cell lysate (Lane 1) and mouse colon tissue lysate (Lane 2).

Specifications

Product Data

• Applications: WB
• Recommended Dilution: ELISA: 1/1,000.
  Western blotting: 1/100 - 1/500.
  Flow cytometry.
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the center region of human CKB.
• Specificity: This antibody reacts to Creatine Kinase BB (CKB).
• Formulation: PBS with 0.09% (W/V) sodium azide
  State: Purified
  State: Liquid purified Ig
• Concentration: lot specific
• Purification: Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: creatine kinase B
• Database Link:
  Entrez Gene 1152 Human
  UniProt ID P12277
• Background: Creatine Kinase (CK) is a dimeric enzyme composed of either M- or B-type subunits. The resulting isoenzymes are expressed at varying levels in different tissues. CK-BB, a cytoplasmic predominantly found in brain tissues, participates in energy homeostasis, reversibly catalyzing the transfer of a phosphate group between ATP and target proteins such as a creatine phosphate. CK-BB exists in normally neglible amounts in the serum of adults, overexpression of CK-BB is associated with cancers of the breast, ovary, prostate, colon, and in small-cell lung cancer. Global assessment of changes in serum levels of CK-BB, CK-MB and CK-MM, are used as a marker for myocardial infarction.
• Synonyms: Creatine kinase B-type, Creatine kinase B chain, B-CK, Creatine kinase BB, CKB