DDIT3 Rabbit Polyclonal Antibody

CAT#: AP06131PU-N

DDIT3 rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of GADD153 / CHOP Antibody at 1/500 dilution: Lane 1: HEK293T cell lysate. Lane 2: Raw264.7 cell lysate. Lane 3: Rat Liver tissue lysate.

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  Immunohistochemistry (IHC) analysis of GADD153 / CHOP Antibody in paraffin-embedded human breast carcinoma tissue.

Specifications

Product Data

• Applications: ELISA, IF, IHC, WB
• Recommended Dilution: Western blot: 1/500-1/1000.
  Immunofluorescence: 1/50-1/200.
  Immunohistochemistry on Paraffin Sections: 1/50-1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide, corresponding to amino acids 100-150 of Human CHOP.
• Specificity: This antibody detects endogenous levels of GADD153/DDIT3 protein.
  (region surrounding Lys121)
• Formulation: Phosphate Buffered Saline (PBS), pH~7.2
  State: Aff - Purified
  State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE)
  Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purification: Affinity chromatography using epitope-specific immunogen
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~26 kDa
• Gene Name: DNA damage inducible transcript 3
• Database Link:
  Entrez Gene 1649 Human
  UniProt ID P35638
• Background: GADD153 has been described as a growth arrest and DNA damageinducible gene that encodes a C/EBP-related nuclear protein. This protein has also been designated C/EBP-homologous protein (CHOP-10). GADD 153 expression is induced by a variety of cellular stresses, inducing nutrient deprivation and metabolic perturbations. GADD153 functions to block cells in G1 to S phase in cell cycle progression and acts by dimerizing with other C/EBP proteins to direct GADD153 dimers away from 'classical' C/EBP binding sites, recognizing instead unique 'nonclassical' sites. Thus GADD153 acts as a negative modulator of C/EBP-like proteins in certain terminally differentiated cells, similar to the regulatory function of Id on the activity of Myo D and Myo D-related proteins involved in the development of muscle cells.
• Synonyms: DDIT3, DDIT-3, CHOP10, CHOP-10