esxB Rabbit Polyclonal Antibody

CAT#: AP03010PU-N

esxB rabbit polyclonal antibody, Aff - Purified

Product Images

Figure 1. Western Blotting analysis (reducing conditions) of recombinant protein CFP10 using polyclonal anti-CFP10 antibody (AP03010PU-N): Lane 1, 3, 5: cell lysates of non-transfected E. coli (negative controls). Lane 2, 4, 6: cell lysate of CFP10-transfe

Specifications

Product Data

• Applications: WB
• Recommended Dilution: Suitable for Western Blotting under Reducing conditions: 0.2-1 μg/ml.
• Reactivities: Mycobacterium tuberculosis
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: CFP10 (Rv3874) of Mycobacterium tuberculosis
• Specificity: The polyclonal antibody reacts with CFP10 (LHP), a 12,3 kDa antigen encoded by gene Rv3874 of Mycobacterium tuberculosis.
  The antibody recognizes CFP10 of Mycobacterium tuberculosis H37Rv and Mycobacterium bovis; it reacts also with recombinant antigen produced in Escherichia coli.
• Formulation: PBS, pH 7.4 with 15 mM sodium azide as preservative.
  State: Aff - Purified
  State: Liquid purified IgG fraction (> 95% pure by SDS-PAGE).
• Concentration: lot specific
• Purification: Protein-A affinity chromatography.
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C.
  DO NOT FREEZE!
  
• Stability: Shelf life: one year from despatch.
• Database Link:
  UniProt ID P0A566
• Background: CFP10 (10 kDa culture filtrate protein) is a low molecular weight protein isolated from Mycobacterium tuberculosis culture filtrates. CFP10 and the 6 kDa early secretory antigen of T cells (ESAT6) are secreted in abundance by Mycobacterium tuberculosis and are frequently recognized by T cells from infected people. The genes encoding these proteins have been deleted from the genome of the vaccine strain Bacille Calmette Guerin (BCG).
  CFP10 is one of a small number of secreted M. tuberculosis proteins now identified, that appear to play key roles in tuberculosis pathogenesis and in the stimulation of immunity.
• Synonyms: lhp, mtsA10, Rv3874, MT3988, MTV027.09