Protein Kinase A regulatory subunit I alpha (PRKAR1A) Rabbit Polyclonal Antibody

CAT#: AP01335PU-N

Protein Kinase A regulatory subunit I alpha (PRKAR1A) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of PKR1 antibody (Cat.-No.: AP01335PU-N) in extracts from COLO cells.

Specifications

Product Data

• Applications: IF, WB
• Recommended Dilution: Western Blot: 1/500-1/1000.
  Immunofluorescence: 1/50-1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Specificity: PKR1 antibody detects endogenous levels of PKR1 protein. (region surrounding Phe58)
• Formulation: Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2.
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: 1.0 mg/ml
• Purification: Affinity chromatography
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~ 45 kDa
• Gene Name: protein kinase cAMP-dependent type I regulatory subunit alpha
• Database Link:
  Entrez Gene 5573 Human
  UniProt ID P10644
• Background: The prokineticin receptors, PKR1 (GPR73a) and PKR2 (GPR73b), are G proteincoupled receptors responsible for mediating the signal transduction of both EG-VEGF and Prokineticin-2. PKR1 and PKR2 share 87% sequence identity. PKR1 is predominantly expressed in the peripheral tissues and PKR2 is typically expressed in the CNS. Both receptors are found in the testis. Upon ligand binding, PKR1 and PKR2 associate with G protein and can promote intracellular calcium mobilization, stimulate phosphoinositide turnover and activate the MAPK pathway. These receptors play a role in a variety of physiological events such as intestinal contraction, ingestive behavior, spermatogenesis, angiogenesis, circadian rhythm, neuronal survival and hyperalgesia. PKR1 may promote cardiomyocyte survival. PKR2 is essential for the normal development of the olfactory bulb. Mutations in the gene encoding PKR2 may result in Kallmann syndrome type 3.
• Synonyms: PKR1, PRKAR1, TSE1, CAR, CNC1