Neutrophil Elastase (ELANE) Mouse Monoclonal Antibody [Clone ID: 265-3K1]

CAT#: AM26361PU-N

Neutrophil Elastase (ELANE) mouse monoclonal antibody, clone 265-3K1

Specifications

Product Data

• Clone Name: 265-3K1
• Applications: ELISA, WB
• Recommended Dilution: Immunoassays.
  Western blot: The typical starting working dilution is 1/50.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Specificity: The monoclonal antibody 265-3K1 recognizes Human Leukocyte Elastase.
• Formulation: PBS
  State: Liquid 0.2 µm filtered Ig fraction
  Stabilizer: 0.1% BSA
  Preservative: 0.02% sodium azide
• Concentration: lot specific
• Purification: Protein G Chromatography
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C.
• Stability: Shelf life: one year from despatch.
• Gene Name: elastase, neutrophil expressed
• Database Link:
  Entrez Gene 1991 Human
  UniProt ID P08246
• Background: Leukocyte elastase, a major serine proteinase in man, is predominantly present in the azurophilic granules of neutrophils and monocytes. Elastase has a broad range of extracellular matrix substrates including elastin, proteoglycans, collagen and fibronectin. The action of elastase is controlled by serine proteinase inhibitors. Elastase, when released during inflammation, is rapidly bound by its two main inhibitors, alpha1-PI and alpha2-macroglobuline to form elastase-inhibitor complexes. In addition mucosa secretions may contain the locally secreted elastase inhibitors elafin/SKALP and SLPI. When secreted at sites of inflammation elastase can cause severe tissue damage. An important role has been suggested for human elastase in various inflammatory disorders, including pulmonary emphysema, sepsis, arthritis, nephritis and certain skin diseases. Elastase induces the production of IL-8 in human bronchial epithelial, a proces that occurs in part through TLR4.
• Synonyms: ELANE, ELA2, Neutrophil elastase, Elastase-2, PMN elastase, Bone marrow serine protease, Medullasin, Human leukocyte elastase, HLE