Twist (TWIST1) Mouse Monoclonal Antibody [Clone ID: 3E10]

CAT#: AM10230PU-N

Twist (TWIST1) mouse monoclonal antibody, clone 3E10, Purified

Specifications

Product Data

• Clone Name: 3E10
• Applications: ELISA, IHC, WB
• Recommended Dilution: ELISA.
  Western Blot: 1/1000-1/5000.
  Immunohistochemistry: 1/100-1/1000.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Synthetic peptide derived from N-term domain of Human Twist-1 protein
• Specificity: Reacts with Human 21 kDa TWIST1 protein.
  Cross reacts with Mouse, Rat and other species.
  It does not cross reacts with Twist-2.
• Formulation: 0.1M Tris, 0.1M Glycine and 2% Sucrose
  State: Purified
  State: Lyophilized powder
  Preservative: None
• Reconstitution Method: Restore with 0.1 ml distilled water to receive a concentration of 1 mg/ml.
• Concentration: lot specific
• Purification: Protein A Chromatography
• Conjugation: Unconjugated
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month
  or (in aliquots) at -20°C long term.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: twist family bHLH transcription factor 1
• Database Link:
  Entrez Gene 7291 Human
  UniProt ID Q15672
• Background: Basic helix-loop-helix (bHLH) transcription factors have been implicated in cell lineage determination and differentiation. TWIST1 is a bHLH transcription factor and shares similarity with another bHLH transcription factor, Dermo1. The strongest expression of this mRNA is in placental tissue, in adults, mesodermally derived tissues express this mRNA preferentially. Mutations in this gene have been found in patients with Saethre-Chotzen syndrome.
• Synonyms: Twist-related protein 1, TWIST, BHLHA38