Bmi1 (COMMD3-BMI1) Mouse Monoclonal Antibody [Clone ID: 3E3]

CAT#: AM06695SU-N

Bmi1 (COMMD3-BMI1) mouse monoclonal antibody, clone 3E3, Ascites

Product Images

Western blot analysis using BMI1 mAb against human BMI1 (AA: 1-326) recombinant protein. (Expected MW is 74 kDa)

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  Western blot analysis using BMI1 mAb against HEK293 (1) and BMI1-hIgGFc transfected HEK293 (2) cell lysate.

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  Red: Control Antigen (100ng) Purple: Antigen (10ng) Green: Antigen (50ng) Blue: Antigen (100ng)

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  Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using BMI1 mouse mAb with DAB staining.

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  Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using BMI1 mouse mAb with DAB staining.

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  Immunofluorescence analysis of Hela cells using BMI1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

• Flow cytometric analysis of NIH/3T3 cells using BMI1 mouse mAb (green) and negative control (red).

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Specifications

Product Data

• Clone Name: 3E3
• Applications: ELISA, FC, IF, IHC, WB
• Recommended Dilution: Western Blot: 1/500 - 1/2000.
  Immunohistochemistry on paraffin sections: 1/200 - 1/1000.
  Immunofluorescence: 1/200 - 1/1000.
  Flow cytometry: 1/200 - 1/400.
  ELISA: 1/10000.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Purified recombinant fragment of human BMI1 expressed in E. Coli.
  
• Specificity: This antibody reacts to BMI1.
• Formulation: State: Ascites
  State: Ascitic fluid containing 0.03% sodium azide.
• Conjugation: Unconjugated
• Storage:

  Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Predicted Protein Size: 37 kDa
• Gene Name: COMMD3-BMI1 readthrough
• Database Link:
  Entrez Gene 100532731 Human
  UniProt ID P35226
• Background: Component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. In the PRC1 complex, it is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2.
• Synonyms: BMI1, PCGF4