CRKL Mouse Monoclonal Antibody [Clone ID: 5E12]

CAT#: AM06643SU-N

CRKL mouse monoclonal antibody, clone 5E12, Ascites

Product Images

Western blot analysis using CRKL mAb against human CRKL (AA: 100-303) recombinant protein. (Expected MW is 60 kDa)

• 

  Red: Control Antigen (100ng) Purple: Antigen (10ng) Green: Antigen (50ng) Blue: Antigen (100ng)

• 

  Flow cytometric analysis of NIH/3T3 cells using CRKL mouse mAb (blue) and negative control (red).

Specifications

Product Data

• Clone Name: 5E12
• Applications: ELISA, FC, WB
• Recommended Dilution: Western Blot: 1/500 - 1/2000.
  Flow cytometry: 1/200 - 1/400.
  ELISA: 1/10000.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Purified recombinant fragment of human CRKL expressed in E. Coli.
  
• Specificity: This antibody reacts to CRKL.
• Formulation: State: Ascites
  State: Ascitic fluid containing 0.03% sodium azide.
• Conjugation: Unconjugated
• Storage:

  Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Predicted Protein Size: 39 kDa
• Gene Name: v-crk avian sarcoma virus CT10 oncogene homolog-like
• Database Link:
  Entrez Gene 1399 Human
  UniProt ID P46109
• Background: This gene encodes a protein kinase containing SH2 and SH3 (src homology) domains which has been shown to activate the RAS and JUN kinase signaling pathways and transform fibroblasts in a RAS-dependent fashion. It is a substrate of the BCR-ABL tyrosine kinase, plays a role in fibroblast transformation by BCR-ABL, and may be oncogenic.
• Synonyms: Crk-like protein