PRMT4 (CARM1) Mouse Monoclonal Antibody [Clone ID: 3H2]

CAT#: AM06565SU-N

PRMT4 (CARM1) mouse monoclonal antibody, clone 3H2, Ascites

Product Images

Western blot analysis using CARM1 antibody Cat.-No AM06565SU-N against MCF-7 (1), Hela (2), NIH/3T3 (3), HL-60 (4), LNcap (5), Jurkat (6), PC-3 (7), Cos7 (8), and PC-12 (9) cell lysate.

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  Immunohistochemical analysis of paraffin-embedded breast cancer tissues (left) and ovarian cancer tissues (right) using CARM1 antibody Cat.-No AM06565SU-N with DAB staining.

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  Immunofluorescence analysis of Hela cells using CRAM1 antibody Cat.-No AM06565SU-N (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

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  Flow Cytometric analysis of Lovo cells using CARM1 antibody Cat.-No AM06565SU-N (green) and negative control (purple).

Specifications

Product Data

• Clone Name: 3H2
• Applications: ELISA, FC, IF, IHC, WB
• Recommended Dilution: ELISA: 1/10000.
  Western Blot: 1/500 - 1/2000.
  Immunofluorescence: 1/200 - 1/1000.
  Flow Cytometry: 1/200 - 1/400.
  Immunohistochemistry on Paraffin Sections: 1/200 - 1/1000.
• Reactivities: Human, Monkey, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Purified recombinant fragment of Human CARM1 expressed in E. Coli.
• Specificity: Recognizes CARM1
• Formulation: State: Ascites
  State: Ascitic fluid containing 0.03% Sodium Azide.
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 65 kDa
• Gene Name: coactivator associated arginine methyltransferase 1
• Database Link:
  Entrez Gene 10498 Human
  UniProt ID Q86X55
• Background: Protein arginine N-methyltransferases, such as CARM1, catalyze the transfer of a methyl group from S-adenosyl-L-methionine to the side chain nitrogens of arginine residues within proteins to form methylated arginine derivatives and S-adenosyl-L-homocysteine. Protein arginine methylation has been implicated in signal transduction, metabolism of nascent pre-RNA, and transcriptional activation (Frankel et al. 2002 (PubMed 11724789). Tissue specificity: Overexpressed in prostate adenocarcinomas and high-grade prostatic intraepithelial neoplasia.
• Synonyms: PRMT4