Clusterin (CLU) Mouse Monoclonal Antibody [Clone ID: Hs-3]

CAT#: AM03078PU-N

Clusterin (CLU) mouse monoclonal antibody, clone Hs-3, Aff - Purified

Product Images

Apolipoprotein J antibody staining of Formalin-Fixed Paraffin-Embedded Human Testis at 10 ug/ml.

Specifications

Product Data

• Clone Name: Hs-3
• Applications: ELISA, IF, IHC, WB
• Recommended Dilution: ELISA.
  Western blot: 1/ µg/ml
  Positive Material: seminal plasma
  Immunocytochemistry.
  Immunohistochemistry on Paraffin Sections: 10 µg/ml.
  Positive Tissue: Sertoli cells.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Freshly ejaculated human sperms were washed in PBS and extracted in 3% acetic acid, 10% glycerol, 30 mM benzaminidine.
  The acid extract was dialyzed against 0.2% acetic acid and subsequently used for immunization.
• Specificity: Recognizes Apolipoprotein J (Apo J).
• Formulation: PBS, pH~7.4 with 15 mM Sodium Azide as preservative
  State: Aff - Purified
  State: Liquid purified IgG fraction (> 95% pure by SDS-PAGE)
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein A
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: clusterin
• Database Link:
  Entrez Gene 1191 Human
  UniProt ID P10909
• Background: Clusterin (APO J, SGP-2, TRPM-2, SP-40, pADHC-9, CLJ, T64, GP III, XIP8) is a 75-80 kD disulfide-linked heterodimeric protein containing about 30% of N-linked carbohydrate rich in sialic acid but truncated forms targeted to the nucleus have also been identified. It is a conserved secreted glycoprotein expressed by a wide range of tissues and being implicated in many physiological processes, including e.g. lipid transportation, complement inhibition, tissue remodeling, membrane recycling, or clearence of cellular debris. It is nearly ubiqitously expressed in most mammalian tissues and can be found in plasma, milk, urine, cerebrospinal fluid and semen. Clusterin is able to bind and form complexes with numerous partners (immunoglobulins, lipids, heparin, bacteria, complement components, paraoxonase, beta amyloid, leptin etc.) and is expressed in many pathological and clinically relevant situations including cancer, organ regeneration, infection, Alzheimer disease, retinitis pigmentosa, myocardial infarction, renal tubular damage, autoimmunity and others. A genuine function of clusterin is still enigmatic.
• Synonyms: Clusterin, APOJ, CLI, KUB1, AAG4, NA1/NA2, SP-40,40