OriGene Technologies, Inc.
Search:    
Left ProductsProducts divider ServicesServices divider technologyTechnology divider researchResearch divider TechsupportTechSupport divider AboutAbout Right
 
Home cDNA Clone TrueORF Lentiviral Vector

Lentiviral system for efficient DNA delivery (Lenti shRNA & LentiORF)

divider

OriGene is licensed to provide lentiviral vectors and related products.

  • Third generation Lentiviral system
  • Ideal for delivering DNA into hard-to-transfect and animal models
  • Replication-incompetent system for safety
  • Improved biosafety features, BL2

Lentiviral Particles Offered New

spacer
lenti particle flow chart

Both shRNA and ORF lentiviral particles are offered!

  • Ready-to-Use: Just add to cells and get results
  • Coverage: Genome-wide, Human, Mouse and Rat
  • Broad spectrum: Transduce dividing, non-dividing cells and in vivo
  • Biosafety: Replication deficient 3rd generation lenti particles

A. LentiORF, Human, Mouse and Rat, Genome-wide

All lentiORF clones are tagged with either C-terminal Myc-DDK tag or mGFP tag.

Clone Finder
Search your clone, type clone name, symbol, accession number or keyword in the box below.
Details of the LentiORF vectors

LentiORF Vectors

  pLenti-C-Myc-DDK pLenti-C-mGFP
Vector SKU PS100064 PS100071
Vector Map pLenti-C-Myc-DDK Vector Map pLenti-C-mGFP Vector Map
Fusion Tag C-terminal Myc-DDK C-terminal mGFP
Ideal For In hard-to-transfect cells: Detection and purification of over-expressed protein Tracking the over-expressed protein in tranfected cells
ORF Clone SKUs RC2XXXXXL1 RC2XXXXXL2

Other than the above 2 Lentiviral vectors, ORF clones housed in which can be purchased directly, OriGene provides a variety of lentiviral destination vectors. The ORF inserts of all TrueORF clones can be shuttled into destination vectors via a simple cut-and-ligate process. (Learn more). OriGene provides cloning service when other destination vectors are preferred

Destination VectorSKUC-TagE. coli SelectionExpressionPrice
pLenti-C-Myc-DDKPS100064Myc-DDKChloramphenicolMammalian$690
pLenti-C-GFPPS100065GFPChloramphenicolMammalian$690
pLenti-C-Myc-DDK-IRES-PuroPS100069Myc-DDKChloramphenicolMammalian$690
pLenti-C-Myc-DDK-IRES-GFPPS100070Myc-DDKChloramphenicolMammalian$690
pLenti-C-mGFPPS100071mGFPChloramphenicolMammalian$690
pLenti-EF1a-C-tGFPPS100072tGFPChloramphenicolMammalian$690
pLenti-N-tGFP PS100073 ChloramphenicolMammalian$690
pLenti-C-tRFPPS100074tRFPChloramphenicolMammalian$690
pLenti-N-tRFPPS100077 ChloramphenicolMammalian$690
pLenti-N-tYFPPS100078 ChloramphenicolMammalian$690
pLenti-C-tYFPPS100079tYFPChloramphenicolMammalian$690
pLenti-C-Myc-DDK-IRES-tRFPPS100080Myc-DDKChloramphenicolMammalian$690
pLenti-C-Myc-DDK-IRES-NeoPS100081Myc-DDKChloramphenicolMammalian$690
pLenti-C-Myc-DDK-IRES-BSDPS100082Myc-DDKChloramphenicolMammalian$690
pLenti-EF1a-C-mGFPPS100084mGFPChloramphenicolMammalian$690
pLenti-EF1a-C-Myc-DDK-IRES-Puro      PS100085Myc-DDKChloramphenicolMammalian$690
pLenti-EF1a-C-Myc-DDK-IRES-GFP     PS100086Myc-DDKChloramphenicolMammalian$690
pLenti-C-Myc-DDK-P2A-tGFPPS100088Myc-DDKChloramphenicolMammalian$690
pAAV-AC-Myc-DDKPS100089Myc-DDKampicilinMammalian$570
pAAV-AC-GFPPS100090tGFPampicilinMammalian$570
pAAV-AC-RFPPS100091tRFPampicilinMammalian$570
pLenti-C-Myc-DDK-P2A-PuroPS100092Myc-DDKChloramphenicolMammalian$690
pLenti-C-mGFP-P2A-PuroPS100093mGFPChloramphenicolMammalian$690

B. Lentiviral shRNAs, Human, Mouse and Rat, Genome-wide

The Lentiviral cassette contains three major functional elements, shRNA, a puromycin selection marker & a tGFP reporter.

shRNA Finder
Search by gene symbol or keyword.
Results include shRNA and siRNA
Details of the Lentiviral shRNA vectors

pGFP-C-shLenti vector

pGFP-C-shLenti vector map

pGFP-C-shLenti vector:

  • Third generation Lentivector
  • Chloramphenicol marker for bacterial selection
  • Three major functional elements within the 5’LTR and 3’LTR. All of them can be packaged to viral particles and transduced to many cell lines.
    1. shRNA expression cassette under U6 promoter
    2. Puromycin resistant for mammalian cell selection
    3. tGFP gene as reporter.

The shRNA expression cassette

  • 29mer hairpin with a 7bp loop.
  • U6 driven expression
  • A Pol III termination sequence (TTTTTT) immediately downstream of the hairpin.
  • The gene-specific shRNA cassette is sequence-verified to ensure its match to the target gene.

OriGene also has another Lentiviral shRNA vector, pRFP-CB-shLenti

C. Lentiviral packaging kit

SKU Description Manual Size Price Availability  
TR30022 Lenti-vpak packaging kit - packaging plasmids and transfection reagent Kits Manual 10 rxns $390 In Stock Add to Shopping Cart
TR30022P5 Lenti-vpak packaging kit - packaging plasmids and transfection reagent, 5 pack of TR30022 Kits Manual 5 x 10 rxns $1590 In Stock Add to Shopping Cart

D. Control Lenti particles and concentrator

shRNA Control Particles

Cat # Description Price Availability  
TR30021V Control Lenti particles, shRNA scramble, expressing GFP and puro, >1x107 TU/ml, 0.5 ml $195 3 Days Add To Cart
TR30021V5 Control Lenti particles, scramble shRNA, expressing GFP and puro, >1x107 TU/ml, 5x0.5 ml $495 3 Days Add To Cart
TR30033V Control Lenti particles, shRNA scramble, expressing RFP and BSD, >1x107 TU/ml, 0.5 ml $195 3 Days Add To Cart
TR30033V5 Control Lenti particles, shRNA scramble, expressing RFP and BSD, >1x107 TU/ml, 5x0.5 ml $495 3 Days Add To Cart

LentiORF Control Particles

Cat # Description Price Availability  
PS100064V LentiORF control particles of pLenti-C-Myc-DDK, >1x107 TU/ml, 0.5 ml $195 3 Days Add To Cart
PS100064V5 LentiORF control particles of pLenti-C-Myc-DDK, >1x107 TU/ml, 5x0.5 ml $495 3 Days Add To Cart
PS100071V LentiORF control particles of pLenti-C-mGFP, >1x107 TU/ml, 0.5 ml $195 3 Days Add To Cart
PS100071V5 LentiORF control particles of pLenti-C-mGFP, >1x107 TU/ml, 5x0.5 ml $495 3 Days Add To Cart

Lenti Concentrator

Cat # Description Price Availability  
TR30025 Lentivirus concentration solution (5X), 50ml $150 In Stock Add To Cart
TR30026 Lentivirus concentration solution (5X), 4x50ml $390 In Stock Add To Cart

Lentivirus related precaution and disclaimers

These products are for R&D use only, not for drug, household or other uses.

Please consult the Material Safety Data Sheet for information regarding the hazards and safe handling practices. Although the lentiviral transduction particles produced are replication incompetent, it is highly recommended that they be treated as Risk Group Level 2 (RGL-2) organisms. Follow all published RGL-2 guideline for handling and waste decontamination.

 

spacer
Inc 5000 Healthcare Company
All Products by: Title | Price | Category | Popularity | Best Sellers Topselling Products by: Title | Price | Category | Popularity | Favorites
Popular Categories: Popularity | Our Choices | All-Round Favorites | Title Topselling Categories: Popularity | Our Choices | All-Round Favorites | Title